RESUMO
Arsenic poisoning in ground water is one of the most sensitive environmental pollutant causing serious pollution all over the world. Chronic arsenic exposure through drinking water to humans leads to major public health related issues. There have been very meagre studies which reported that, the plant constituents proved to exhibit protective effect from arsenicosis. Therefore, the present study aims to evaluate the protective efficacy of Coriandrum sativum seeds extract against sodium arsenite induced toxicity in Swiss albino mice. In the present study twenty-four male healthy Swiss albino mice (30 ± 5 g) were divided into four groups (n = 6), where the control group received normal diet and water; group II and group III treated with sodium arsenite (2 mg per kg body weight per day) for 2 and 4 weeks respectively. The group IV mice were administered with C.sativum seeds extract at the dose of 150 mg per kg body weight per day for 4 weeks upon sodium arsenite pretreated (2 mg/kg body weight for 4 weeks per day) mice. After the complete dose duration, all the treatment group animals were sacrificed same day for haematological, biochemical and histopathological study. In the arsenic treated mice, there were significant (p < 0.0001) changes in the serum levels of ALT, AST, ALP, urea, uric acid and creatinine as well as in the haematological parameters. In contrast, after the administration with C.sativum seeds extract upon arsenic pretreated mice, there was significant (p < 0.0001) improvement observed in the hepatic and renal biomarker parameters as well as haematological variables. In the arsenic intoxicated mice, after administration with C.sativum seeds extract there was significant (p < 0.0001) reduction in the arsenic concentration in blood, liver and kidney tissues as well as in the serum LPO levels. Furthermore, the histopathological study showed that, C.sativum seeds extract administrated group of mice significantly restored the liver and kidney at cellular level against arsenic induced toxicity. The entire study concludes that C.sativum seeds extract possesses the ameliorative effect against arsenic induced liver and kidney intoxication.
RESUMO
The present research designed to assess the protective role of Salvia officinalis essential oil (SO) against carbon tetrachloride (CCl4)-induced liver and kidney damage in mice. This is evidenced by estimation of antiradical scavenging activity of SO using DPPH assay, biochemical markers, histological investigation of liver and kidney sections, and comet assay. Mice were given CCl4 (1.2 mL/kg for 24 h or 0.8 mL/kg for 2 weeks, 3 times/week) and with or without SO (0.1, 0.2, and 0.4 mL/kg, for 2 week, 5 times/week). The findings demonstrated that both acute and subacute treatment with CCl4 alone had adverse side effects on liver and kidney of mice. These effects were evidenced by a significant increase in serum hepatic enzymes (ALT, AST, ALP, LDH, and G-GT), bilirubin, and renal function markers (blood urea, creatinine). Toxic effect of CCl4 was accompanied by a decline in the serum total protein, albumin, globulin, and prothrombin (%). CCl4 induced oxidative stress as evidenced by increasing serum lipid peroxidation (LPO) along with decreasing serum total glutathione S transferase (GST). A remarkable increase in hepatic DNA strand breakages and histopathological distortion in liver and kidney specimens were observed in CCl4-intoxicated groups. Ultrastructurally, hepatocytes exhibited irregular nuclei, vacuolated cytoplasm, and distorted microorganelles. Essential oil form S. officinalis possessed antiradical scavenging (EC50 = 4602 µg/mL) lower than ascorbic acid (EC50 = 5.9 µg/mL). This oil was effectively exhibited hepato-nephroprotective activity especially at its higher concentrations in co-treated groups (SO plus CCl4). The activity of SO was associated with lowering the liver enzymes, bilirubin, urea, and creatinine, along with increasing total protein, albumin, globulin, and prothrombin. The increase in GST content and the decrease in LPO and DNA breakage levels, alongside repairing the histo-architectural distortions further confirmed the protective activity of SO. SO is a potential candidate for counteracting hepato/renal injury associating CCl4. This effect may occur via antioxidant defense mechanism which in part related to the complexity of its chemical constituents.