Suppression of Phytophthora capsici in Chile Pepper Using Brassica juncea and Hordeum vulgare Cover Crop Residues and Trichoderma harzianum as a Biocontrol Agent.

Phytophthora blight, caused by Phytophthora capsici, is a serious disease of many vegetable crops worldwide. In New Mexico, U.S.A., the disease affects chile pepper (Capsicum annuum L.), a major crop in the state. There is no single tool that effectively controls the disease. Continuous research is needed in identifying combination of tools that can reduce the impact of Phytophthora blight. We explored the potential of combining cover crops and biocontrol agents to reduce soilborne diseases. This study aimed to evaluate the effects of Indian mustard (Brassica juncea L.) cover crop on the antagonistic ability of Trichoderma harzianum against P. capsici in vitro and to quantify the impacts of combining soil amendment with residues from B. juncea and barley (Hordeum vulgare L.) cover crops and plastic covering on infection of chile pepper seedlings by P. capsici under greenhouse conditions. Volatiles from macerated tissue of B. juncea significantly reduced P. capsici and T. harzianum growth in the absence of soil by 89.0 and 79.0%, respectively. When incorporated in soils, volatiles from macerated tissue of B. juncea significantly reduced P. capsici and T. harzianum by 33.4 and 7.8%, respectively. T. harzianum was more resilient to B. juncea biofumigation than P. capsici. Significant reduction in disease incidence was observed with B. juncea-fumigated soil, while no disease suppression was observed with soil incorporation of H. vulgare residues. Covering soil with plastic was necessary for increasing the efficacy of B. juncea biofumigation.

The effect of layers of high tunnel covering and soil mulching on tomato fruit quality.

BACKGROUND: High tunnels (HT) and plastic mulch can increase yield, extend growing seasons, protect plants from harsh weather conditions, and produce more marketable and attractive tomatoes. However, plastic covering reduces solar radiation, which may affect the quality of tomatoes. This study investigated the effects of single-layer versus double-layer HT and plastic mulch versus bare soil on the quality of fully ripe tomato fruits. The study was conducted over 2 years (2021 and 2022). The fruit color (L*, a*, and b*), vitamin C, pH, total titratable acid (TTA), total soluble solids (TSS), and lycopene content of the two tomato cultivars (Brandywine and Rebelski) were quantified. RESULTS: The results showed that quality parameters varied with the plastic layers of HT, plastic mulch, and planting year. The double-layer HT only showed a slight but significant influence on the color of tomatoes of both cultivars in the same year (P < 0.05). Brandywine tomatoes grown in the double-layer HT had significantly lower pH and lycopene content than those grown in single-layer HT, regardless of mulching. The effect of plastic layers on TTA, TSS, and lycopene depended on whether the soil was mulched. Tomatoes grown on bare soil had higher TTA and TSS values than those grown on mulched soil in double-layer HT. Tomatoes grown in single-layer HTs had significantly higher vitamin C content than those in the double-layer HT for both cultivars regardless of mulching. CONCLUSION: This study demonstrates that double-layer HT is unnecessary for the improvement of the overall quality of tomatoes. © 2023 Society of Chemical Industry.

Incidence of bacterial diseases associated with irrigation methods on onions (Allium cepa).

BACKGROUND: In the last decade, diseases of bacterial origin in onions have increased and this has led to significant losses in production. These diseases are currently observed in both the Old and New Worlds. The present study aimed to evaluate whether the irrigation method influences the incidence of diseases of bacterial origin. RESULTS: In cases where the inoculum was natural, the initial incidence of soft bacterial rot did not manifest in any treatment in the first year, whereas, at the end of the conservation period, all treatments had increased incidences of infection. Sprinkler irrigation (8%) was statistically differentiated from the other treatments, for which the final incidence was similar (4.5%). For all irrigation treatments, the final incidence of bacterial soft rot decreased or remained stable towards the end of the cycle, with the exception of sprinkler irrigation in 2015, which increased. CONCLUSION: From the results of the present study, it can be inferred that the irrigation method does have an influence on the incidence of diseases of bacterial origin in the post-harvest stage for onions. © 2018 Society of Chemical Industry.

Methyl Bromide Alternatives for Control of Root-knot Nematode (Meloidogyne spp.) in Tomato Production in Florida.

The following work was initiated to determine the scope of application methodology and fumigant combinations for increasing efficacy of 1,3-dichloropropene (1,3-D) and metam sodium for management of root-knot nematodes (Meloidogyne spp.) in Florida. A series of five experiments were set up during spring and fall seasons to evaluate the potential of different fumigants, alone or in combination, in polyethylene film tomato production. The most promising chemical alternatives to methyl bromide, in terms of root-knot nematode management, were the combinations 1,3-D-chloropicrin, chloropicrin-proprietary solvent ,and 1,3-D-metam sodium. Sprayed or injected metam sodium generally provided only short-term nematode management and by harvest nematode infection was not different from the nontreated control. Drip-applied metam sodium gave good nematode management under high nematode pressure, but needs further verification to establish (i) the importance of soil moisture and temperature on treatment efficacy and (ii) whether similar management can be obtained with fewer than three drip tubes. Broadcast applications of 1,3-D showed better efficacy as compared to applications on a preformed raised bed. Fumigation did not increase tomato yields in spring when root-knot nematode pressure was low, but during fall all chemical treatments increased yields three to five-fold, as root-knot nematode was a major yield-limiting factor.

Impact of repeated fumigant applications on soil properties, crop yield, and microbial communities in a plastic-mulched tomato production system.

Pre-plant soil fumigation is widely applied to control nematodes, soil-borne fungal pathogens, and weeds in vegetable crops. However, most of the research evaluating the effect of fumigants on crop yield and soil microbial communities has been done on single compounds despite growers mainly applying fumigant combinations. We studied the effect of different fumigant combinations (chloropicrin, 1,3-dichloropropene, and metam potassium) on soil properties, crop yield, and the soil bacterial and fungal microbiome for two consecutive years in a plastic-mulched tomato production system in Florida (United States). While combinations of fumigants did not improve plant productivity more than the individual application of these products, application of fumigants with >60 % chloropicrin did significantly increase yield. Fumigant combinations had no significant effect on bacterial diversity, but fumigants with >35 % chloropicrin reduced soil fungal diversity and induced temporary changes in the soil bacterial and fungal community composition. These changes included short-term increases in the relative abundance of Firmicutes and Ascomycota, as well as decreases in other bacterial and fungal taxa. Repeated fumigation reduced network complexity and the relative abundance of several predicted bacterial functions and fungal guilds, particularly after fumigation and at end of harvest (3-months post fumigation). A structural equation model (SEM) showed fumigants not only directly impact crop yield, but they can also indirectly determine variations in plant productivity through effects on the soil microbiome. Overall, this study increases our understanding of the environmental and agricultural impacts of fumigants in a plastic-mulched tomato production system.

Microplastics in vineyard soils: First insights from plastic-intensive viticulture systems.

In the terrestrial environment, microplastics in specialty cropping systems have not been studied so far. Viticulture as a potential plastic-intensive management form and a land use with high erosion risks, plays an important role in transport and distribution of material to other terrestrial and aquatic systems. This paper is a first investigation of microplastics in vineyard soils, assessing the spatial distribution and composition of microplastics in organically and conventionally managed viticulture, and relates it to the macroplastic collected at the vineyards. Topsoils (0-10, 10-30 cm) and plastic particles on soil surfaces from eight vineyard lots were sampled. Four of the vineyards were under organic and four underconventional management and they were all located in the Moselle and Saar Wine Region (Rhineland-Palatinate, Germany). Microplastic analysis was performed via µFTIR chemical imaging after wet-chemical microplastic extraction from soil samples. The mean microplastic concentration was 4200 ± 2800 p kg-1 (mean ± SD), with detected mean sizes of 230 µm ± 300 µm. Most abundant polymers were PP (35.2 %), PA (25.3 %) and PE (15.5 %). The distribution pattern showed higher microplastic concentration in topsoil, at middle and bottom slope position. The smallest particle sizes were found in subsoil samples and bottom position. Thus, erosion is assumed to be a potential downhill transport pathway. According to our dataset, management seems to have no significant influence on microplastic abundance, but affects polymer composition. Polymer composition of micro- and macroplastics partly coincide, thus in-situ fragmentation, is considered the major input source. Based on our findings, we recommend further investigation of plastic pathways in speciality crop systems like viticulture.

Formation, behavior, properties and impact of micro- and nanoplastics on agricultural soil ecosystems (A Review).

Micro and nanoplastics (MPs and NPs, respectively) in agricultural soil ecosystems represent a pervasive global environmental concern, posing risks to soil biota, hence soil health and food security. This review provides a comprehensive and current summary of the literature on sources and properties of MNPs in agricultural ecosystems, methodology for the isolation and characterization of MNPs recovered from soil, MNP surrogate materials that mimic the size and properties of soil-borne MNPs, and transport of MNPs through the soil matrix. Furthermore, this review elucidates the impacts and risks of agricultural MNPs on crops and soil microorganisms and fauna. A significant source of MPs in soil is plasticulture, involving the use of mulch films and other plastic-based implements to provide several agronomic benefits for specialty crop production, while other sources of MPs include irrigation water and fertilizer. Long-term studies are needed to address current knowledge gaps of formation, soil surface and subsurface transport, and environmental impacts of MNPs, including for MNPs derived from biodegradable mulch films, which, although ultimately undergoing complete mineralization, will reside in soil for several months. Because of the complexity and variability of agricultural soil ecosystems and the difficulty in recovering MNPs from soil, a deeper understanding is needed for the fundamental relationships between MPs, NPs, soil biota and microbiota, including ecotoxicological effects of MNPs on earthworms, soil-dwelling invertebrates, and beneficial soil microorganisms, and soil geochemical attributes. In addition, the geometry, size distribution, fundamental and chemical properties, and concentration of MNPs contained in soils are required to develop surrogate MNP reference materials that can be used across laboratories for conducting fundamental laboratory studies.

Plastics derived from disposable greenhouse plastic films and irrigation pipes in agricultural soils: a case study from Turkey.

Plastics are ubiquitous. It has been used in human activities, from agriculture to packaging, infrastructure, and health. The wide range of usage makes plastics an omnipresent pollutant in the environment. This study investigated the abundance and type of plastics in agricultural soil in the Adana/Karatas region in Turkey, where disposable low-tunnel greenhouse plastic films and irrigation pipes were in use. For this purpose, 1 kg of soil samples from the top 5 cm (from the surface) was taken from 10 different sampling locations. An average of 16.5 ± 2.4 pcs/kg was found in the soil samples. The highest amount of plastics was seen at the Bahçe-4 location with 39.7 ± 12 pcs/kg and the lowest amount of plastics at the Karatas-1 location with 0.7 ± 0.3 pcs/kg. The average size of plastics was found to be 18.2 ± 1.3 mm. The average size of plastics originating from greenhouse cover was 18.9 ± 1.4 mm, and from disposable irrigation pipes was 12.5 ± 3.5 mm. It was determined that 41.9% of extracted plastics were microplastics, 36.3% were mesoplastics, 16.3% were macroplastics, and 5.6% were megaplastics. Results indicated that residual plastics decreased in the soil where used plastics were removed after usage. As a result, it is worth noting that a significant amount of plastics remain in soil due to plastics being used in agricultural areas.

Sustainable production of healthy, affordable food in the UK: The pros and cons of plasticulture.

An evolving green agenda as the UK seeks to achieve 'net zero' in greenhouse gas emissions by 2050, coupled with our new trading relationship with the European Union, is resulting in new government policies, which will be disruptive to Britain's traditional food and farming practices. These policies encourage sustainable farming and land-sparing to restore natural habitats and will provide an opportunity to address issues such as high emissions of GHGs and dwindling biodiversity resulting from many intensive agricultural practices. To address these and other food challenges such as global conflicts and health issues, Britain will need a revolution in its food system. The aim of this paper is to make the case for such a food revolution where additional healthy food for the UK population is produced in-country in specialised production units for fruits and vegetables developed on sites previously considered unsuitable for crop production. High crop productivity can be achieved in low-cost controlled environments, making extensive use of novel crop science and modern controlled-environment technology. Such systems must be operated with very limited environmental impact. In recent years, growth in the application of plasticulture in UK horticulture has driven some increases in crop yield, quality and value. However, the environmental cost of plastic production and plastic pollution is regarded as a generational challenge that faces the earth system complex. The distribution of plastic waste is ubiquitous, with a significant pollution load arising from a range of agricultural practices. The primary receptor of agriplastic pollution is agricultural soil. Impacts of microplastics on crop productivity and quality and also on human health are only now being investigated. This paper explores the possibility that we can mitigate the adverse environmental effects of agriplastics and thereby exploit the potential of plasticulture to enhance the productivity and positive health impact of UK horticulture.

Soil erosion as transport pathway of microplastic from agriculture soils to aquatic ecosystems.

Soil erosion is a potentially important source of microplastic (MP) entering aquatic ecosystems. However, little is known regarding the erosion and transport processes of MP from agricultural topsoils. The aim of this study is to analyze the erosion and transport behavior of MP during heavy rainfall events, whereas a specific focus is set to preferential MP transport and MP-soil interactions potentially leading to a more conservative transport behavior. The study is based on a series of rainfall simulations on paired-plots (4.5 m × 1.6 m) of silty loam and loamy sand located in Southern Germany. The simulations (rainfall intensity 60 mm h-1) were repeated 3 times within 1.5 years. An amount of 10 g m-2 of fine (MPf, size 53-100 µm) and 50 g m-2 of coarse (MPc, size 250-300 µm) high-density polyethylene as common polymer was added to the topsoil (<10 cm) of the plots. The experiments show a preferential erosion and transport of the MP leading to a mean enrichment ratio of 3.95 ± 3.71 (MPc) and 3.17 ± 2.58 (MPf) in the eroded sediment. There was a higher MP enrichment on the loamy sand but a higher sediment delivery on the silty loam resulting in nearly equal MP deliveries from both soil types. An increasing interaction with mineral soil particles or aggregates leads to a decreasing MP delivery over time. Within 1.5 years, up to 64% of the eroded MP particles were bound to soil particles. Overall, more of the MPc was laterally lost via soil erosion, while for the MPf the vertical transport below the plough layer was more important. In general, our study indicates that arable land susceptible to soil erosion can be a substantial MP source for aquatic ecosystems.

Urinary phthalate metabolites among workers in plastic greenhouses in western China.

Agricultural plastic greenhouse (PG) production can extend the growing season of crops to satisfy domestic consumption in countries such as China. Workers in PGs have potential higher phthalate exposure risks than the general population as phthalate accumulation has been observed in greenhouse soil, air, and crops. To date, biomonitoring tests of phthalates for the working population have not been carried out. To address this shortage, we conducted a pilot study in Shaanxi Province, China, among 35 healthy PG workers by follow-up recording their seasonal dietary habits and work activities and urine sample collection and measurement between 2018 and 2019. The objectives were to uncover the association between phthalate metabolites and the population characteristics, seasonal and diurnal variations and causes, and to estimate exposure risks and contributions of exposure pathways from PG production systems. A total of 13 phthalate metabolite concentrations (Σ13 phthalate metabolites) ranged from 102 to 781 (5th-95th) ng/mL (median: 300 ng/mL). Mono-n-butyl phthalate (MNBP) made up 51.3% of Σ13 phthalate metabolites, followed by the sum of four di-2-ethylhexyl phthalate (DEHP) metabolites (24.2%), mono-2-isobutyl phthalate (MIBP) (13.4%), and mono-ethyl phthalate (MEP) (9.8%). The concentrations of MNBP and MIBP in summer were significantly higher than the levels in winter (p < 0.0001). A total of 62.3% of the PG worker population was shown to have exposure risks, and the proportion was as high as 79.4% in summer. Phthalate exposure of the workers from PG production systems constituted over 20% of the total creatinine-based daily intake, and consuming vegetables and fruit planted in PGs and inhalation in PGs were the two largest exposure pathways. Our findings demonstrate that it is important to protect workers in PGs from phthalate exposure risks, and phasing out the use of plastic materials containing phthalates in PGs is imperative, to guarantee food safety in PGs.

Effects of biodegradable plastic film mulching on soil microbial communities in two agroecosystems.

Plastic mulch films are used globally in crop production but incur considerable disposal and environmental pollution issues. Biodegradable plastic mulch films (BDMs), an alternative to polyethylene (PE)-based films, are designed to be tilled into the soil where they are expected to be mineralized to carbon dioxide, water and microbial biomass. However, insufficient research regarding the impacts of repeated soil incorporation of BDMs on soil microbial communities has partly contributed to limited adoption of BDMs. In this study, we evaluated the effects of BDM incorporation on soil microbial community structure and function over two years in two geographical locations: Knoxville, TN, and in Mount Vernon, WA, USA. Treatments included four plastic BDMs (three commercially available and one experimental film), a biodegradable cellulose paper mulch, a non-biodegradable PE mulch and a no mulch plot. Bacterial community structure determined using 16S rRNA gene amplicon sequencing revealed significant differences by location and season. Differences in bacterial communities by mulch treatment were not significant for any season in either location, except for Fall 2015 in WA where differences were observed between BDMs and no-mulch plots. Extracellular enzyme assays were used to characterize communities functionally, revealing significant differences by location and sampling season in both TN and WA but minimal differences between BDMs and PE treatments. Overall, BDMs had comparable influences on soil microbial communities to PE mulch films.

Plastic film mulching increased the accumulation and human health risks of phthalate esters in wheat grains.

Plastic film mulching is a common practice to increase crop yield in dryland, while the wide use of plastic film has resulted in ubiquitous phthalate esters (PAEs) releasing into the soil. PAEs in soil could be taken up and accumulated by dietary intake of food crops such as wheat, thus imposing health risks to residents. In the present study, samples from a long-term location-fixed field experiment were examined to clarify the accumulation of PAEs in soil and wheat, and to assess the human health risks from PAEs via dietary intake of wheat grain under plastic film mulching cultivation in dryland. Results showed that concentrations of PAEs in grains from mulching plots ranged from 4.1 to 12.6 mg kg-1, which were significantly higher than those in the control group. There was a positive correlation for the PAE concentrations between wheat grains and field soils. Concentrations of PAEs in the soil were in the range of 1.8-3.5 mg kg-1 for the mulching treatment, and 0.9-2.7 mg kg-1 for the control group. Di-n-butyl phthalate (DBP) and di-(2-ethylhexyl) phthalate (DEHP) were detected in all soil and grain samples, and DEHP was found to be the dominant PAE compound in grains. Based on DEHP concentrations in wheat grains, the values of carcinogenic risk for adults were higher than the recommended value 10-4. Results indicated that wheat grains from film mulching plots posed a considerable non-carcinogenic risk to residents, with children being the most sensitive resident group. Findings of this work call the attention to the potential pollution of grain crops growing in the plastic film mulching crop production systems.

Enhancement in the Physico-Mechanical Functions of Seaweed Biopolymer Film via Embedding Fillers for Plasticulture Application-A Comparison with Conventional Biodegradable Mulch Film.

This study aimed to compare the performance of fabricated microbially induced precipitated calcium carbonate⁻ (MB⁻CaCO3) based red seaweed (Kappaphycus alvarezii) bio-polymer film and commercial calcium carbonate⁻ (C⁻CaCO3) based red seaweed bio-film with the conventional biodegradable mulch film. To the best of our knowledge, there has been limited research on the application of commercial CaCO3 (C⁻CaCO3) and microbially induced CaCO3 (MB⁻CaCO3) as fillers for the preparation of films from seaweed bio-polymer and comparison with biodegradable commercial plasticulture packaging. The results revealed that the mechanical, contact angle, and biodegradability properties of the polymer composite films incorporated with C⁻CaCO3 and MB⁻CaCO3 fillers were comparable or even superior than the conventional biodegradable mulch film. The seaweed polymer film incorporated with MB⁻CaCO3 showed the highest contact angle of 100.94°, whereas conventional biodegradable mulch film showed a contact angle of 90.25°. The enhanced contact angle of MB⁻CaCO3 resulted in high barrier properties, which is highly desired in the current scenario for plasticulture packaging application. The water vapor permeability of MB⁻CaCO3 based seaweed films was low (2.05 ± 1.06 g·m/m²·s·Pa) when compared to conventional mulch film (2.68 ± 0.35 g·m/m²·s·Pa), which makes the fabricated film an ideal candidate for plasticulture application. The highest tensile strength (TS) was achieved by seaweed-based film filled with commercial CaCO3 (84.92% higher than conventional mulch film). SEM images of the fractured surfaces of the fabricated films revealed the strong interaction between seaweed and fillers. Furthermore, composite films incorporated with MB⁻CaCO3 promote brighter film, better water barrier, hydrophobicity, and biodegradability compared to C⁻CaCO3 based seaweed polymer film and conventional mulch film. From this demonstrated work, it can be concluded that the fabricated MB⁻CaCO3 based seaweed biopolymer film will be a promising candidate for plasticulture and agricultural application.

Survival of Didymella bryoniae in Infested Muskmelon Crowns in South Carolina.

The crowns of muskmelon (Cucumis melo subsp. melo) plants are susceptible to cankers caused by the fungal pathogen Didymella bryoniae. The objective of this study was to compare the length of time D. bryoniae survived in infested crowns that were buried or left on the soil surface. Dried crowns with cankers were buried 12.5 cm deep, placed on the soil surface, or placed on top of raised beds covered with white-on-black polyethylene mulch from July 2002 to June 2003, December 2003 to October 2004, July 2004 to November 2005, and November 2005 to November 2007. At regular intervals, crowns or crown debris were retrieved, washed, cut into pieces, and cultured on semiselective medium to recover D. bryoniae. D. bryoniae was not recovered from crowns buried 35 and 45 weeks in 2003 and 2004 but was recovered from 2.5% of crowns buried 66 weeks in 2005. In contrast, D. bryoniae was recovered after 48, 45, 66, and 103 weeks from 66, 6.3, 2.5, and 10% of crowns on the soil surface in 2003, 2004, 2005, and 2007, respectively. D. bryoniae also was recovered after 66 and 103 weeks from 12.5 and 8% of crowns on mulched beds in 2005 and 2007. In two additional experiments, the pathogen was recovered from 15.0 and 20.1% of infested muskmelon debris left in place for 42 and 38 weeks on polyethylene-mulched beds. To reduce the time D. bryoniae survives after a cucurbit crop, crop debris should be incorporated into soil promptly after harvest.

Plastic mulching in agriculture. Trading short-term agronomic benefits for long-term soil degradation?

Plastic mulching has become a globally applied agricultural practice for its instant economic benefits such as higher yields, earlier harvests, improved fruit quality and increased water-use efficiency. However, knowledge of the sustainability of plastic mulching remains vague in terms of both an environmental and agronomic perspective. This review critically discusses the current understanding of the environmental impact of plastic mulch use by linking knowledge of agricultural benefits and research on the life cycle of plastic mulches with direct and indirect implications for long-term soil quality and ecosystem services. Adverse effects may arise from plastic additives, enhanced pesticide runoff and plastic residues likely to fragment into microplastics but remaining chemically intact and accumulating in soil where they can successively sorb agrochemicals. The quantification of microplastics in soil remains challenging due to the lack of appropriate analytical techniques. The cost and effort of recovering and recycling used mulching films may offset the aforementioned benefits in the long term. However, comparative and long-term agronomic assessments have not yet been conducted. Furthermore, plastic mulches have the potential to alter soil quality by shifting the edaphic biocoenosis (e.g. towards mycotoxigenic fungi), accelerate C/N metabolism eventually depleting soil organic matter stocks, increase soil water repellency and favour the release of greenhouse gases. A substantial process understanding of the interactions between the soil microclimate, water supply and biological activity under plastic mulches is still lacking but required to estimate potential risks for long-term soil quality. Currently, farmers mostly base their decision to apply plastic mulches rather on expected short-term benefits than on the consideration of long-term consequences. Future interdisciplinary research should therefore gain a deeper understanding of the incentives for farmers and public perception from both a psychological and economic perspective in order to develop new support strategies for the transition into a more environment-friendly food production.

Increased occurrence of pesticide residues on crops grown in protected environments compared to crops grown in open field conditions.

Crops grown under plastic-clad structures or in greenhouses may be prone to an increased frequency of pesticide residue detections and higher concentrations of pesticides relative to equivalent crops grown in the open field. To test this we examined pesticide data for crops selected from the quarterly reports (2004-2009) of the UK's Pesticide Residue Committee. Five comparison crop pairs were identified whereby one crop of each pair was assumed to have been grown primarily under some form of physical protection ('protected') and the other grown primarily in open field conditions ('open'). For each pair, the number of detectable pesticide residues and the proportion of crop samples containing pesticides were statistically compared (n=100 s samples for each crop). The mean concentrations of selected photolabile pesticides were also compared. For the crop pairings of cabbage ('open') vs. lettuce ('protected') and 'berries' ('open') vs. strawberries ('protected') there was a significantly higher number of pesticides and proportion of samples with multiple residues for the protected crops. Statistically higher concentrations of pesticides, including cypermethrin, cyprodinil, fenhexamid, boscalid and iprodione were also found in the protected crops compared to the open crops. The evidence here demonstrates that, in general, the protected crops possess a higher number of detectable pesticides compared to analogous crops grown in the open. This may be due to different pesticide-use regimes, but also due to slower rates of pesticide removal in protected systems. The findings of this study raise implications for pesticide management in protected-crop systems.

Produtividade e qualidade da framboeseira 'Batum' cultivada sobcobertura plástica e dois espaçamentos em região subtropical / Production and quality of raspberries 'Batum' cultivated under cover plastic and two spacing in subtropical area

Framboesa é uma pequena fruta de grande importância econômica, que contém numerosos compostos fenólicos, com potenciais benéficos à saúde, porém, quando cultivada em regiões subtropicais possui baixa produtividade. Objetivou-se, neste trabalho, avaliar a produtividade de framboeseiras 'Batum', produzidas com ou sem cobertura plástica sobre o dossel das plantas, em duas densidades de plantio, sendo 0,25m e 0,5m entre plantas e 3,0m entre linhas e avaliadas por dois ciclos de produção (2012/13 e 2013/14), em região subtropical. O delineamento experimental adotado foi em blocos ao acaso, em esquema fatorial 2x2x2, com 6 blocos e 10 plantas por parcela. Foram avaliadas as características produtivas como número de frutos por planta, produção por planta e produtividade estimada. Além disso, foram demarcados os índices fenológicos, análises vegetativas e fisiológicas. O menor espaçamento de plantio entre as plantas (0,25m) e o plantio sem cobertura plástica sobre o dossel das plantas resultaram em maior produtividade das framboseiras 'Batum'.

Raspberry is an economically important berry crop, its fruit contains numerous phenolic compounds with potential health benefits, however ver, in subtropical areas has been low production. The objective of this study was to evaluate the productivity of raspberry 'Batum' produced with or without plastic cover over the plant canopy and two planting densities, being 0.25m and 0.5m between plants and 3.0m between rows and cycles (2012/13 and 2013/14), in subtropical area. The experimental design was randomized blocks, factorial 2x2x2 with 6 blocks and 10 plants per plot. It was evaluated the productive plant characteristics such as number of fruits per plant, production per plant and productivity estimated. Moreover,phenological data, vegetative and physiologic analysis. The planting densities at 0.25m between plants and without plastic cover over the plant canopy result in increased productivity of raspberry 'Batum'.

Manejo de videiras sob cultivo protegido / Grapevines management under protected cultivation

O cultivo protegido na cultura da videira apresenta-se como uma alternativa na diminuição da incidência de doenças fúngicas em regiões que apresentam excesso de chuvas no período da maturação. A utilização de cobertura plástica sobre as fileiras de plantas ocasiona modificações no microclima junto às videiras. Essas alterações propiciam condições favoráveis ao crescimento e incremento da produtividade. Todavia, desfavorecem o desenvolvimento de doenças fúngicas, como as podridões de cachos, que atualmente é um dos maiores problemas no controle fitossanitário na "Serra Gaúcha", reduzindo a necessidade de fungicidas. O uso de fungicidas nessas condições merece muita cautela, devido à redução de radiação ultravioleta e ausência de chuvas sobre os cachos, pelo uso da cobertura plástica, que prolonga o período residual dos fungicidas. O maior acúmulo e a persistência são preocupantes, tanto nas uvas destinadas ao consumo in natura, que afeta diretamente o consumidor, quanto nas destinadas à vinificação, em que prejudica a atuação das leveduras na fermentação dos vinhos. De forma geral, a tecnologia de cobrir os vinhedos com filmes plásticos é eficaz no controle de doenças e na redução do uso de fungicidas. Contudo, deve ser considerada como um novo sistema de produção, principalmente por exigir um manejo fitossanitário distinto em relação ao cultivo convencional.

Protected grapevines cultivation is an alternative to decrease incidence of fungal disease in some regions with excess rain during the ripening period. Plastic covering use above rows cause modifications in plant's microclimate. Theses alterations propitiated by protected cultivation can be promotes increase in plant development and yield. Plastic covering is unfavorable for fungal disease development as clusters rot, nowadays, an important problem in healthy handling in Serra Gaúcha Region, reducing the need of sprays. Another point in plastic covering is the necessity of careful fungicides use; due to ultraviolet reduction radiation and rain absence above clusters, which prolongs the fungicide residual period. This higher residual accumulation in grapes is dangerous for consumption in natura, which directly affects the consummator as well as vinification process, interfering in yeast in wine's fermentation. In general, this technology has efficiency in control fungal disease, however, another production system need to be considered, mainly because requires a different management when compared to the conventional production.

Microclima de vinhedos sob cultivo protegido / Microclimate of vineyards under protected cultivation

Alterações microclimáticas em vinhedos, provocadas pelo uso de cobertura plástica, interferem na fisiologia das plantas e na incidência de doenças fúngicas em videiras. Assim, o objetivo deste trabalho foi avaliar a influência da cobertura plástica no microclima de vinhedos, em particular na qualidade da radiação solar. O experimento foi conduzido nos ciclos 2005/06 e 2006/07, em Flores da Cunha, Rio Grande do Sul (RS), em um vinhedo de 'Moscato Giallo' conduzido em "Y", com cobertura plástica impermeável (160µm) sobre 12 fileiras com 35m, deixando-se cinco fileiras sem cobertura (controle). Em ambas as áreas, avaliou-se o microclima quanto à temperatura do ar, umidade relativa do ar, radiação fotossinteticamente ativa e velocidade do vento, próximo ao dossel vegetativo e aos cachos. Medições contínuas foram efetuadas utilizando sensores e sistemas automáticos de aquisição de dados. Alterações na qualidade da radiação solar incidente sobre o dossel vegetativo, no espectro de 300 a 750nm, foram avaliadas por meio de medições durante cinco dias, com espectroradiômetro. A cobertura plástica impermeável à água sobre as fileiras das plantas aumentou a temperatura do ar e diminuiu a radiação fotossinteticamente ativa e a velocidade do vento. A cobertura interferiu na qualidade da radiação solar incidente, principalmente, reduzindo a irradiância na faixa do ultravioleta e a razão entre a radiação nas faixas do vermelho e vermelho-distante.

Microclimate alterations promoted by plastic covering over vineyards interfere in the plant physiology and fungal diseases incidence on grapevines. The aim of this research was to evaluate the influence of the plastic covering on the microclimate of vineyards, in particular on the quality of the incoming solar radiation. The experiment was carried out in 2005/06 and 2006/07 seasons in Flores da Cunha-RS, in a vineyard of Moscato Giallo cultivar shaped in Y, with impermeable plastic (160µm) over 12 rows of 35m length and five rows without covering (control). In both treatments the air temperature and humidity, incoming photosynthetically radiation and wind speed were measured at the level of the canopy and clusters. Continuing measurements were taken through sensors and automatic acquisition systems (datalogger). Influences of the covering on quality of the incoming solar radiation, from 300 to 750nm, were evaluated through a spectroradiometer. The impermeable plastic covering above the plant rows increased the air temperature and decreased the photosynthetically radiation and wind speed. The covering interfered on the quality of the incoming solar radiation, by reducing mainly the irradiance in the ultraviolet band and reducing also the ratio between the irradiance in the red and far-red bands.