Multi-pollutant removal dynamics by aquatic plants in monoculture or mixed communities.

Much of our knowledge about the phytoremediation potential of floating treatment wetlands (FTWs) comes from studies focusing on the removal of single pollutants, often by a single plant species. Here, we quantify the potential of FTWs planted with varying proportions of the emergent monocots Typha latifolia, Glyceria maxima, and Phragmites australis to simultaneously remove a suite of eleven nutrient/metalloid pollutants. Pollutants most readily removed from water included total inorganic nitrogen (TIN), K and Mn, whilst P, Zn and Cu showed a moderate removal efficiency, and Mg, Ca, Na, Cr, and Fe were poorly removed. Root length within a FTW was correlated with lower concentrations of remaining in the water, whilst plant uptake and tissue sequestration was more important for reducing concentrations of Mn, TIN, P, and Fe. The effect of community composition over time was greatest for the removal of Zn, with FTWs containing T. latifolia having the strongest effect; community type was less important for the removal of TIN, Mg, K, and Na. Plant tissue sequestration was important for reducing concentrations of Mn, TIN, P and Fe in the water, with median uptake values all greater than 12.5%. Importantly, the removal of some pollutants (e.g., Cu) increased with retention time. Therefore, depending on the management objective, FTWs generally perform better where and when residence times are longer e.g., in ponds or streams under low flow, and assembling FTW communities with varying traits and associated removal mechanisms can allow several pollutants to be remediated at once.

Effect of monocultures and polycultures of Typha latifolia and Heliconia psittacorum on the treatment of river waters contaminated with landfill leachate/domestic wastewater in partially saturated vertical constructed wetlands.

Partially Saturated Vertical Constructed Wetlands (PSV-CWs) are novel wastewater treatment systems that work through aerobic and anaerobic conditions that favor the removal of pollutants found in high concentrations, such as rivers contaminated with domestic wastewater and landfill leachate. The objective of the study was to evaluate the efficiency of PSV-CWs using monocultures and polycultures of Typha latifolia and Heliconia psittacorum to treat river waters contaminated with leachates from open dumps and domestic wastewater. Six experimental units of PSV-CWs were used; two were planted with Typha latifolia monoculture, two with Heliconia psittacorum monoculture and two with polycultures of both plants. The results indicated better organic matter and nitrogen removal efficiencies (p < 0.05) in systems with polycultures (TSS:95%, BOD5:83%, COD:89%, TN:82% and NH4+:99%). In general, the whole system showed high average removal efficiencies (TSS:93%, BOD5:79%, COD:85%, TN:79%, NH4+:98% and TP:85%). Regarding vegetation, both species developed better in units with monocultures, being Typha latifolia the one that reached a more remarkable development. However, both species showed high resistance to the contaminated environment. These results showed higher removals than those reported in the literature with conventional Free Flow Vertical Constructed Wetlands (FFV-CWs), so PSV-CWs could be a suitable option to treat this type of effluent.

The research addresses the contamination of water resources in developing countries by landfill leachate and domestic wastewater discharges. It proposes treatment through Partially Saturated Vertical Constructed Wetlands (PSV-CWs), which, despite the limited information available, have been shown to be effective in removing pollutants in effluents with high concentrations. In addition to evaluating PSV-CWs, the study examines the impact of different types of vegetation on pollutant removal efficiency, concluding that PSV-CWs are a promising and viable option for the treatment of these effluents.

Investigating the effect of hydraulic residence time, artificial aeration and plants presence on different constructed wetland designs treating oil industry effluent.

Constructed Wetlands (CW) have gained popularity over the last decades due to their cost-effectiveness, easy and simple operation and environmental compatibility in wastewater treatment. This ecological engineering technology appears particularly ideal for low-income regions. In this study, three widely used CW types (horizontal flow, vertical flow, and hybrid CW) were constructed and evaluated for their effectiveness in removing various pollution parameters (BOD5, COD, TSS, NH4-N, NO3-N, and TP) from an industrial effluent. Different configurations were tested such as CW type, hydraulic residence time, plants presence, and artificial aeration. Results showed that the hybrid CW configuration (i.e., vertical flow CW followed by horizontal subsurface flow CW) achieved the highest removal rates of all pollutants, i.e., more than 90% of BOD5, COD, TSS, and NH4-N. The single horizontal flow and vertical flow CW designs showed variations in the removal of NO3-N and TP (less than 30%), which were significantly improved (50% and 70%, respectively) by using the hybrid CW system. Artificial aeration significantly improves the performance of the CW system, especially for ammonia nitrogen and organic matter removal, while plants presence is also beneficial in the treatment performance. An 8-days HRT seems to be adequate for high removal rates in passive CW designs, though in aerated wetlands a lower HRT of 4 days seems sufficient. These findings suggest that the hybrid CW system could be a promising option for efficient wastewater treatment in developing regions.

Immobile Iron-Rich Particles Promote Arsenic Retention and Regulate Arsenic Biotransformation in Treatment Wetlands.

Remediation of arsenic (As)-contaminated wastewater by treatment wetlands (TWs) remains a technological challenge due to the low As adsorption capacity of wetland substrates and the release of adsorbed As to pore water. This study investigated the feasibility of using immobile iron-rich particles (IIRP) to promote As retention and to regulate As biotransformation in TWs. Iron-rich particles prepared were immobilized in the interspace of a gravel substrate. TWs with IIRP amendment (IIRP-TWs) achieved a stable As removal efficiency of 63 ± 4% over 300 days, while no As removal or release was observed in TWs without IIRP after 180 days of continuous operation. IIRP amendment provided additional adsorption sites and increased the stability of adsorbed As due to the strong binding affinity between As and Fe oxides. Microbially mediated As(III) oxidation was intensified by iron-rich particles in the anaerobic bottom layer of IIRP-TWs. Myxococcus and Fimbriimonadaceae were identified as As(III) oxidizers. Further, metagenomic binning suggested that these two bacterial taxa may have the capability for anaerobic As(III) oxidation. Overall, this study demonstrated that abiotic and biotic effects of IIRP contribute to As retention in TWs and provided insights into the role of IIRP for the remediation of As contamination.

Treatment of naphthenic acids in oil sands process-affected waters with a surface flow treatment wetland: mass removal, half-life, and toxicity-reduction.

This study is the first to investigate the removal of naphthenic acids in a full-scale constructed wetland within the Alberta Oil Sands region. The average mass-removal efficiency for all O2-naphthenic acids measured in three separate deployments in the wetland ranged from 7.5% to 68.9% and appeared sensitive to physicochemical properties of the naphthenic acids, environmental conditions, and water quality. Treatment efficiency of individual naphthenic acids was found to increase with increasing carbon number and decreasing number of double bond equivalents in the molecule. Treatment efficiency was also found to increase with both higher initial turbidity in OSPW entering the wetland, and warmer average OSPW temperatures during wetland operation. Half-life times of naphthenic acids in the treatment wetland ranged between 8.9 and 39 days and were substantially lower than those in tailings ponds (i.e., 12.9-13.6 years) and laboratory studies focussed on bench-scale aerobic microbial biodegradation (i.e., 44-315 days). Using published dose-response data, biomimetic extraction measurements using solid phase microextraction fibers indicate that 14 days of wetland treatment resulted in a reduction in (4 d) deformity of Danio rerio from 50 to 16%, while exhibiting less than 1% toxic response for less sensitive toxic endpoints. The study concludes that wetland treatment is a feasible and productive treatment method for naphthenic acids in oil sands process-affected water due to a combination of sorption and biodegradation.

Cr, Ni, and Zn removal from landfill leachate using vertical flow wetlands planted with Typha domingensis and Canna indica.

Chromium (Cr), Nickel (Ni), and zinc (Zn) removal from landfill leachate using mesocosm-scale vertical flow wetlands, the effect of recirculation, and the ability of macrophytes to retain metals were evaluated. Wetlands were filled with coarse sand and light expanded clay aggregates and planted with Typha domingensis or Canna indica. Wetlands were operated using intermittent loading, with and without recirculation. Raw leachate was diluted and spiked with metals to reach the following concentrations: 0.2 mg L-1 Cr , 0.2 mg L-1 Ni, and0.2 mg L-1 Zn and 1.0 mg L-1 Cr, 1.0 mg L-1 Ni, and 1.0 mg L-1 Zn. Wetlands planted with T. domingensis presented higher metal removal than those planted with C. indica. Recirculation enhanced metal removal efficiencies significantly, being for T. domingensis/C. indica: 60/54, 49/47, 61/47% for Cr, Ni, and Zn at 0.2 mg L-1, and 80/71, 76/62, 73/59% for Cr, Ni, and Zn at 1.0 mg L-1, respectively. Metals were efficiently retained by macrophytes. Plant biomass and metal concentrations in roots were significantly higher than in shoots. Scanning electron microscopy and X-ray microanalysis showed that metals were absorbed by internal root tissues. A hybrid wetland planted with T. domingensis may be implemented to improve not only metal but also chemical oxygen demand and total nitrogen removals.

Optimizing floating treatment wetland and retention pond design through random forest: A meta-analysis of influential variables.

Floating treatment wetlands (FTWs), artificial systems constructed from buoyant mats and planted with emergent macrophytes, represent a potential retrofit to enhance the dissolved nutrient removal performance of existing retention ponds. Treatment occurs as water flows through the dense network of roots suspended in the water column, providing opportunities for pollutants to be removed via filtration, sedimentation, plant uptake, and adsorption to biofilms in the root zone. Despite several recent review articles summarizing the growing body of research on FTWs, FTW design guidance and strategies to optimize their contributions to pollutant removal from stormwater are lacking, due in part to a lack of statistical analysis on FTW performance at the field scale. A meta-analysis of eight international FTW studies was performed to investigate the influence of retention pond, catchment, and FTW design characteristics on effluent concentrations of nutrients and total suspended solids (TSS). Random forest regression, a tree-based machine learning approach, was used to model complex interactions between a suite of predictor variables to identify design strategies for both retention ponds and FTWs to enhance treatment of nutrient and sediment. Results indicate that pond design features, especially loading ratio and pond depth (which should be limited to 200:1 and 1.75 m, respectively), are most influential to effluent water quality, while the benefits of FTWs were limited to improving mitigation of phosphorus species and TSS which was primarily influenced by FTW coverage and planting density. Findings from this work inform wet retention pond and FTW design, as well as guidance on scenarios where FTW implementation is most appropriate, to improve dissolved nutrient and sediment removal in urban runoff.

Nutrient Removal by Floating Treatment Wetlands Under Different Spatial Arrangement Modes: a Field Study.

Floating treatment wetlands (FTWs) are a management method to improve urban rivers, but most studies have been carried out at laboratory, micro, and meso levels, so it is necessary to study full-scale FTWs as a method to improve urban water bodies. In this experiment, the purification effects of water temperature (WT), dissolved oxygen (DO), ammonia nitrogen (NH4+-N), nitrate nitrogen (NO3--N), total phosphorus (TP), chemical oxygen demand (CODMn), and chlorophyll-a (Chl-a) under staggered arrangement (SA) and centered arrangement (CA) were evaluated. It was found that the DO concentration and removal rate of CODMn, Chl-a, and TP in the SA were significantly higher than those in the CA in months with heavy rainfall. However, interestingly, for TP, August showed the opposite trend. The removal rates of NH4+-N and NO3--N were significantly different throughout the test period. The biomass growth values of shoots and roots of plants in the FTWs were 0.40 ± 0.03 kg/m2 and 1.38 ± 0.07 kg/m2 in the SA and 0.32 ± 0.07 kg/m2 and 1.26 ± 0.30 kg/m2 in the CA. The increments of N absorbed by plants in the SA and CA were 7.08 ± 0.49 g/kg and 6.83 ± 0.07 g/kg, respectively, and the increments of P were 0.57 ± 0.02 g/kg and 0.32 ± 0.07 g/kg, respectively, which indicated that the growth status of plants in the FTWs in the SA was slightly better than that in the CA. In summary, the hybrid-constructed FTWs of both arrangements can effectively improve the water quality of urban rivers, and the effect of the SA was greater than that of the CA. The purification effect of in situ tests under different arrangement modes of hybrid-constructed FTWs was evaluated, which provides guidance and support for the field layout of FTWs in rivers in the future.

Constructed wetlands applied in rural sanitation: A review.

This systematic literature review aimed at presenting experiences on the use of constructed wetlands (CW) as an alternative for the treatment of domestic wastewater in rural areas worldwide. CW units are often preceded by a pre-treatment step, although systems comprising arrangements of CW with different flow types are also applied. The literature review showed that the most commonly treatment system used in rural areas comprised septic tanks followed by CW. Overall, CW rural sanitation systems have shown to consistently remove pollutants, with median removal efficiencies equal to 87% for TSS, 89% for COD, 93% for BOD, 70% for Ntotal and 72% for Ptotal. Removal rates of indicator bacteria of up to 4.0 log10 have also been reported. Recent studies have shown CW to be efficient at removing hormones, pharmaceutical compounds and toxicity of wastewater. Consequently, final effluents are often in compliance with effluent discharge and wastewater reuse regulations. The adoption of pre-treatment reduces CW area requirements and clogging issues, and planted CW present benefits in terms of the removal of pollutants including pathogens. Low implementation and operational costs, simplified operation and maintenance, and high-quality final effluents favour CW. Guidelines provided by the local, competent authorities may support the rural application of CW. Finally, CW systems comprise a promising, sustainable solution for rural sanitation which may support access to adequate and equitable sanitation to several people as well as safe wastewater recycling and reuse, as encouraged by UN Sustainable Development Goal 6, Targets 3 and 4.

Establishment and potential use of woody species in treatment wetlands.

Plant species selection is an important criterion for improving treatment wetland performance. The aim of this work was to evaluate removal efficiency and potential uses of woody species in treatment wetlands during the establishment year. Plant development, removal efficiency and evapotranspiration rate of five woody species (Salix interior, Salix miyabeana, Sambucus canadensis, Myrica gale, Acer saccharinum) and four herbaceous taxa typically used in treatment wetlands (Typha angustifolia, Phragmites australis australis, Phragmites australis americanus, Phalaris arundinacea) were compared in a mesocosm-scale study during one growing season. Woody species showed significantly slower growth, but displayed several characteristics of interest for treatment wetland applications: good adaptation to wetlands conditions; high organic matter removal (76-88%); high nutrient accumulation in tissues and high evapotranspiration capacity. During the establishment year, herbaceous species showed greater biomass development (above- and belowground parts), higher evapotranspiration rate (>3.84 L m-2 d-1 compared to <3.23 L m-2 d-1 for woody species) and overall pollutant removal efficiency. These characteristics confirm the high efficiency of treatment wetlands planted with herbaceous species even in the first growing season. However, given their greater potential biomass development, woody species could represent an excellent alternative for improving treatment wetlands long-term performance.

Plant traits related to the heavy metal removal capacities of wetland plants.

Plants are the crucial component of floating treatment wetlands (FTWs). However, heavy metal removal capacity varies between plant species, and the relationships between plant traits and differences in removal capacity remain unclear. This study sought to determine: (1) the relationships between plant traits and removal of Cd, Cu, Pb, and Zn from water, and (2) the relationships between the removal patterns of these metals. Plants of 34 wetland plant species were exposed to heavy metal concentrations common in stormwater for five days, and 20 traits were measured on each plant. Results indicate that the most important plant traits for heavy metal removal from water are transpiration and high total biomass, especially large amounts of fine roots and leaves. The same traits were generally related to removal both initially and after longer exposure, with stronger correlations found after longer exposure. Plant removal of one metal was likely correlated with removal of the other metals, and the plant removal capacity after 30 min of exposure was correlated with the removal capacity five days later. The present results can be used in selecting plants for enhanced heavy metal removal by FTWs and in identifying additional useful plant species, allowing adaptation to local conditions.

Occurrence and distribution of five antibiotic resistance genes during the loading period in sludge treatment wetlands.

The objectives of this study were to clarify the distribution as well as the removal mechanism of antibiotic resistance genes (ARGs) within three sludge treatment wetlands (STWs) during a loading period of two years. Three STW units were constructed and run during the loading period: Unit 1 (U1) built with aeration tubes, Unit 2 (U2) built with aeration tubes and reeds, and Unit 3 (U3) built with reeds only. All targeted ARGs, intI1, and 16S rRNA were detected in residual sludge in the order of magnitude: 16S rRNA>sul1>intI1>sul2>tetC>tetA>ermB. The abundance of the five targeted ARGs, intI1, and 16S rRNA increased in residual sludge, during the loading period, which may be due to the increase in bacteria caused by the continuous import of exogenous nutrients. However, STWs can also remove ARGs from sewage during the loading period and the mean removal efficiency of five resistance genes was 73.0%. The removal rates of intI1 and 16S rRNA were 73.5% and 78.6%, respectively. Positive correlations were detected in abundance of most ARGs and intI1, as well as 16S rRNA (P < 0.05), indicating intI1 plays a vital part in the propagation of ARGs. The removal of bacteria harboring these genes also occurs in the STW units.

Deciphering of organic matter and nutrient removal and bacterial community in three sludge treatment wetlands under different operating conditions.

Sludge treatment wetlands (STWs) can effectively stabilize sludge, but the microbial community structure in this process is not well characterized. The purpose of this study was to investigate the characteristics of organic matter and nutrient removal and bacterial community in sludge treatment wetlands for treating sewage sludge. Three STWs units included unit STW1 with aeration tubes, unit STW2 with aeration tubes and reed planting and unit STW3 with reed planting. The degradation of organic matter and nutrient, sludge dewatering performance and microbial community dynamics in STWs were examined in feeding and resting periods. Our results showed that during the entire process of the experiment, total solids (TS) in STWs increased to 24-31%, volatile solids (VS) in STWs reduced to 43-47%, while the total kjeldahl nitrogen (TKN) and total phosphorous (TP) concentrations in STWs decreased to 25.1-35.5 mg/g d. w and 5.4-6.2 mg/g d. w. However, the removal efficiencies of organic matter and nutrient in STWs in the feeding period were higher than those in the resting period. Meanwhile, unit STW2 has the best removal performance in organic matter and nutrients during the whole experiment. Microbial community analysis using Illumina MiSeq sequencing technology showed that growth of plants in STWs improved bacterial diversity and richness which corresponded to high removal rates of organic matter and nutrient. Besides, principal coordinate analysis (PCoA) showed that the bacterial community composition in STWs obviously altered between the feeding and the resting periods.

Floating treatment wetlands as biological buoyant filters for wastewater reclamation.

Floating treatment wetlands (FTWs) are an innovative product of ecological engineering that can play a promising role in wastewater treatment. It provides low-cost, eco-friendly, and sustainable solutions for the treatment of wastewater, particularly in regions with economic constraints. Generally, FTWs comprise rooted plants that grow on the surface of water with their roots extending down into the pelagic zone rather than being embedded into the sediments. This drooping structure helps develop (1) a hydraulic flow between the root network and the bottom of the treatment system and (2) a large biologically active surface area for the physical entrapment (filtration) of contaminants, as well as their biochemical transformation and degradation. Furthermore, the rooted network allows proliferation of microorganisms that form biofilms and enhance pollutant degradation while promoting plant growth. The augmentation of bacteria in FTWs has been proven to be the most effective approach for reclamation of wastewater. This article discusses the operational parameters of FTWs for maximal remediation of wastewater and highlights the importance of plant-bacteria partnerships in a typical FTW system for enhanced cleanup of wastewater. We propose that this technology is preferable over other methods that require high energy, costs, and area to install or operate machinery.

Seasonal dynamics of bacterial communities associated with antibiotic removal and sludge stabilization in three different sludge treatment wetlands.

In this study, antibiotics removal, sludge stabilization and the change in the bacterial community in sludge treatment wetlands (STWs) were investigated in different seasons. Pilot-scale STWs were characterized for sludge stabilization and the fate of antibiotics in surplus sludge applied during different seasons in three different configurations. The three configurations were unit S1 with ventilation, unit S2 with ventilation and reed plantings and unit S3 with reed plantings. The antibiotics used were ciprofloxacin, azithromycin and oxytetracycline and their degradation, degree of sludge stabilization and bacterial community dynamics were monitored. The results showed that the removal of antibiotics and reduction in the amount of organics in the planted units S2 and S3 were higher than those in the unplanted unit S1, especially in summer. The antibiotic removal efficiency in the planted unit S2, which was equipped with aeration tubes, was the highest over the entire test period. Bacterial community was analyzed by IlluminaMiSeq sequencing of the 16SrRNA gene, showed that the presence of plants in STWs enhanced microbial diversity and richness which promote the removal of antibiotics and sludge stabilization. Proteobacteria, Bacteroidetes and Firmicutes were dominant in the bacterial communities, with Thiobacillus, Dechloromonas and Pseudomonas occurring as dominant genera.

Enhanced degradation of phenol in floating treatment wetlands by plant-bacterial synergism.

Phenol is a commonly found organic pollutant in industrial wastewaters. Its ecotoxicological significance is well known and, therefore, the compound is often required to be removed prior to discharge. In this study, plant-bacterial synergism was established in floating treatment wetlands (FTWs) in an attempt to maximize the removal of phenol from contaminated water. A common wetland plant, Typha domingensis, was vegetated on a floating mat and augmented with three phenol-degrading bacterial strains, Acinetobacter lwofii ACRH76, Bacillus cereus LORH97, and Pseudomonas sp. LCRH90, to develop FTWs for the remediation of water contaminated with phenol. All of the strains are known to have phenol-reducing properties, and grow well in FTWs. Results showed that T. domingensis was able to remove a small amount of phenol from the contaminated water; however, bacterial augmentation enhanced the removal potential significantly, i.e., 0.146 g/m2/day vs. 0.166 g/m2/day, respectively. Plant biomass also increased in the presence of bacterial consortia; and inoculated bacteria displayed successful colonization/survival in the rhizosphere, root interior and shoot interior of the plant. Similarly, highest reduction in chemical oxygen demand (COD), biochemical oxygen demand (BOD5), and total organic carbon (TOC) was achieved by the combined application of plants and bacteria. The study demonstrates that the plant-bacterial synergism in a FTW may be a more effective approach for the remediation of phenol-contaminated water.

The role of constructed wetlands in a new circular economy, resource oriented, and ecosystem services paradigm.

Wastewater management is included in one of the 17 Sustainable Development Goals (SDGs): SDG 6 is dedicated to water and sanitation and sets out to "ensure availability and sustainable management of water and sanitation for all". SDG 6 expands the Millennium Development Goals (MDGs) focus on drinking water and basic sanitation to now cover the entire water cycle, including the management of water, wastewater and ecosystem resources. A UN report in 2017 states that likely over 80% of the wastewater worldwide is still discharged without adequate treatment. In several countries the wastewater management is nowadays a norm, but still there are open discussions about the kind of approach to be adopted, i.e. centralisation vs. decentralisation. The choice of the adopted technologies is strictly linked to environmental performances and economical aspects; one of the possible causes for the still enormous amount of untreated wastewater discharged into the environment can be the low "willingness to pay" for this kind of service and therefore a great focus should be given to all the technologies that are able to lower the treatment costs still maintaining reliable and robust performances in the long term. When considering wastewater as a carrier of valuable primary chemicals that can be easily converted to marketable products (fertilisers, bio-plastics, soil conditioners, biofuels, etc.), and as well as a relevant source of "new water" to be used for specific purposes, wastewater and runoff management can be highlighted as one of the most exciting challenges and occasions for a sustainable development in the near future. The paper aims to clarify the future role of CWs in circular economy, resource-oriented, and ecosystem services approaches, which want to respond to sanitation worldwide and the future research needs. We give an overview on how the conventional wastewater treatment scheme (what we call "waste paradigm") should move towards more sustainable water and biogeochemical cycles following the new resource-oriented, circular economy and ecosystem service views. On this basis, we review the potential application of CWs within this new, and needed, paradigm. Finally, a meta-analysis shows that the scientific community involved in CWs should put more effort in making CWs more suitable for these new tasks.

Factors affecting the performance of horizontal flow constructed treatment wetland vegetated with Cyperus papyrus for municipal wastewater treatment.

The effect of hydraulic loading rate (HLR) and hydraulic retention time (HRT) on the bioremediation of municipal wastewater using a pilot scale subsurface horizontal flow constructed treatment wetland (HFCTW) vegetated with Cyprus papyrus was investigated. Different HLRs were applied to the treatment system namely 0.18, 0.10, and 0.07 m3/m2. d with corresponding HRTs of 1.8, 3.2, and 4.7 days, respectively. The flow rate was 8 m3/d, and the average organic loading rate (OLR) was 0.037 kg BOD/m3/d. Results showed that the performance of the HFCTW was linearly affected by decreasing the HLR and increasing the HRT. The highest treatment efficiency was achieved at HRT (4.7 days) and HLR (0.07 m3/m2. d). The percentage reductions of chemical oxygen demand (COD), biochemical oxygen demand (BOD), and total suspended solids (TSS) were 86%, 87%, and 80%, respectively. Satisfactory nutrient removal was obtained. Also, removal of 2-3 logs of bacterial indicators of pollution was achieved. The dry biomass of Cyperus was 7.7 kg/m2 and proved to be very efficient in nitrification processes due to high diversity of the roots that increase the treatment surface area.

Impact of abiotic stressors on nutrient removal and rhizomicrobiome composition in floating treatment wetland with Equisetum hyemale.

Floating treatment wetlands (FTW) are receiving growing interest as a phyto-technology. However, there are significant research gaps regarding the actual role of plant species and plant-microbiome interactions. In this study, the nutrient uptake of Equisetum hyemale was examined in FTW microcosms under the influence of abiotic stressors: As (3 mg/L) and Pb (3 mg/L) as well as Cl- (300 and 800 mg/L) in reference to a control during a short screening experiment. High removal efficiency of nutrients in water solutions, up to 88 % for TN and 93 % for PO4-P, was observed. However, PO4-P removal was inhibited in the As reactor, with a maximum efficiency of only 11 %. Lead and As were removed with high efficiency, reaching 98 % and 79 % respectively. At the same time only Pb was effectively bound to root biomass, reaching up to 51 %. Limited As accumulation of 0.5 % in plant roots suggests that microbial processes play a major role in its reduction. The development and structure of microbiome in the microcosms was analysed by means of 16S rRNA gene amplicon sequencing, proving that Pb was the most influential factor in terms of selection pressure on specified bacterial groups. In the As treatment, the emergence of a Serratia subpopulation was observed, while the Cl- treatment preserved a rhizobiome composition most closely resembling the control. This study indicates that E. hyemale is a suitable species for use in FTWs treating Pb polluted water that at the same time is capable to withstand periodic increases in salinity. E. hyemale exhibits low As binding in biomass; however, extended exposure might amplify this effect because of the slow-acting, but beneficial, mechanism of As uptake by roots and shoots. Microbiome analysis complements insights into mechanisms of FTW performance and impact of stress factors on bacterial structure and functions.

Constructed wetland mesocosms improve the biodegradation of microcystin-LR and cylindrospermopsin by indigenous bacterial consortia.

Cyanobacterial blooms releasing harmful cyanotoxins, such as microcystin (MC) and cylindrospermopsin (CYN), are prominent threats to human and animal health. Constructed wetlands (CW) may be a nature-based solution for bioremediation of lake surface water containing cyanotoxins, due to its low-cost requirement of infrastructure and environmentally friendly operation. There is recent evidence that microcystin-LR (MC-LR) can efficiently be removed in CW microcosms where CYN degradation in CW is unknown. Likewise, the mechanistic background regarding cyanotoxins transformation in CW is not yet elucidated. In the present study, the objective was to compare MC-LR and CYN degradation efficiencies by two similar microbial communities obtained from CW mesocosms, by two different experiments setup: 1) in vitro batch experiment in serum bottles with an introduced CW community, and 2) degradation in CW mesocosms. In experiment 1) MC-LR and CYN were spiked at 100 µg L-1 and in experiment 2) 200 µg L-1 were spiked. Results showed that MC-LR was degraded to ≤1 µg L-1 within seven days in both experiments. However, with a markedly higher degradation rate constant in the CW mesocosms (0.18 day-1 and 0.75 day-1, respectively). No CYN removal was detected in the in vitro incubations, whereas around 50 % of the spiked CYN was removed in the CW mesocosms. The microbial community responded markedly to the cyanotoxin treatment, with the most prominent increase of bacteria affiliated with Methylophilaceae (order: Methylophilales, phylum: Proteobacteria). The results strongly indicate that CWs can develop an active microbial community capable of efficient removal of MC-LR and CYN. However, the CW operational conditions need to be optimized to achieve a full CYN degradation. To the best of our knowledge, this study is the first to report the ability of CW mesocosms to degrade CYN.