1.
Chem Pharm Bull (Tokyo)
; 70(3): 199-201, 2022 Mar 01.
Artigo
em Inglês
| MEDLINE
| ID: mdl-34937844
RESUMO
MS is a powerful methodology for chemical screening to directly quantify substrates and products of enzymes, but its low throughput has been an issue. Recently, an acoustic liquid-handling apparatus (Echo®) used for rapid nano-dispensing has been coupled to a high-sensitivity mass spectrometer to create the Echo® MS system, and we applied this system to screening of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) 3CL protease inhibitors. Primary screening of 32033 chemical samples was completed in 12 h. Among the hits showing selective, dose-dependent 3CL-inhibitory activity, 8 compounds showed antiviral activity in cell-based assay.
Assuntos
Tratamento Farmacológico da COVID-19 , Inibidores de Proteases , Acústica , Ensaios de Triagem em Larga Escala/métodos , Humanos , Inibidores de Proteases/química , Inibidores de Proteases/farmacologia , SARS-CoV-2
2.
Biomed Chromatogr
; 36(2): e5278, 2022 02.
Artigo
em Inglês
| MEDLINE
| ID: mdl-34741338