Antimicrobial mechanism and the effect of atmospheric pressure N2 plasma jet on the regeneration capacity of Staphylococcus aureus biofilm.

This study systematically assessed the inactivation mechanism on Staphylococcus aureus biofilms by a N2 atmospheric-pressure plasma jet and the effect on the biofilm regeneration capacity from the bacteria which survived, and their progenies. The total bacterial populations were 7.18 ± 0.34 log10 CFU ml-1 in biofilms and these were effectively inactivated (>5.5-log10 CFU ml-1) within 30 min of exposure. Meanwhile, >80% of the S. aureus biofilm cells lost their metabolic capacity. In comparison, ∼20% of the plasma-treated bacteria entered a viable but non-culturable state. Moreover, the percentage of membrane-intact bacteria declined to ∼30%. Scanning electron microscope images demonstrated cell shrinkage and deformation post-treatment. The total amount of intracellular reactive oxygen species was observed to have significantly increased in membrane-intact bacterial cells with increasing plasma dose. Notably, the N2 plasma treatment could effectively inhibit the biofilm regeneration ability of the bacteria which survived, leading to a long-term phenotypic response and dose-dependent inactivation effect on S. aureus biofilms, in addition to the direct rapid bactericidal effect.

Analysis of Hazy Ga- and Zr-Co-Doped Zinc Oxide Films Prepared with Atmospheric Pressure Plasma Jet Systems.

Co-doped ZnO thin films have attracted much attention in the field of transparent conductive oxides (TCOs) in solar cells, displays, and other transparent electronics. Unlike conventional single-doped ZnO, co-doped ZnO utilizes two different dopant elements, offering enhanced electrical properties and more controllable optical properties, including transmittance and haze; however, most previous studies focused on the electrical properties, with less attention paid to obtaining high haze using co-doping. Here, we prepare high-haze Ga- and Zr-co-doped ZnO (GZO:Zr or ZGZO) using atmospheric pressure plasma jet (APPJ) systems. We conduct a detailed analysis to examine the interplay between Zr concentrations and film properties. UV-Vis spectroscopy shows a remarkable haze factor increase of 7.19% to 34.8% (+384%) for the films prepared with 2 at% Zr and 8 at% Ga precursor concentrations. EDS analysis reveals Zr accumulation on larger and smaller particles, while SIMS links particle abundance to impurity uptake and altered electrical properties. XPS identifies Zr mainly as ZrO2 because of lattice stress from Zr doping, forming clusters at lattice boundaries and corroborating the SEM findings. Our work presents a new way to fabricate Ga- and Zr-co-doped ZnO for applications that require low electrical resistivity, high visible transparency, and high haze.

Increasing xylanase activity of Bacillus subtilis by atmospheric pressure plasma jet for biomass hydrolysis.

Xylanase producing bacteria, Bacillus subtilis, was bombarded by an atmospheric pressure plasma jet (APPJ) and screened for higher catalytic activity. The bacteria were bombarded with plasma of argon or helium with energy of 120 W for a duration of 1-5 min. A mutant with higher xylanase activity was observed under argon plasma treatment at 1 min on media containing xylan as substrate. Subsequently, the xylanase gene from the mutant was sequenced and named MxynA. Sequence analysis revealed only a single missense mutation on the MxynA gene causing amino acid substitution from threonine to serine at position 162 (T162S) within the xylanase protein of the mutant. Consequently, MxynA was subcloned into expression vector, pETDuet-1 under T7 promoter and expressed in E. coli BL21 (DE3). The optimum temperature and pH of MxynA and its parent expressed in E. coli, named CxynA were 60 °C and pH 5, respectively. Moreover, MxynA showed higher xylanase activity approximately 4 fold higher than that of the control upon a wide range of pH and temperature conditions. From kinetic parameters analysis, the mutant showed higher enzyme turnover (k cat) than the control. The hydrolysis ability of the MxynA enzyme on lignocellulosic wastes, such as rice straw, corncob and para grass was investigated using the released reducing sugar as an indicator. The MxynA enzyme showed a greater amount of reducing sugar released from all lignocellulosic wastes other than the control, particularly from para grass. This study demonstrated that the T162S mutation possibly improved the catalytic efficiency of MxynA.

Atmospheric Pressure Microwave Plasma Jet for Organic Thin Film Deposition.

In this work, the potential of a microwave (MW)-induced atmospheric pressure plasma jet (APPJ) in film deposition of styrene and methyl methacrylate (MMA) precursors is investigated. Plasma properties during the deposition and resultant coating characteristics are studied. Optical emission spectroscopy (OES) results indicate a higher degree of monomer dissociation in the APPJ with increasing power and a carrier gas flow rate of up to 250 standard cubic centimeters per minute (sccm). Computational fluid dynamic (CFD) simulations demonstrate non-uniform monomer distribution near the substrate and the dependency of the deposition area on the monomer-containing gas flow rate. A non-homogeneous surface morphology and topography of the deposited coatings is also observed using atomic force microscopy (AFM) and SEM. Coating chemical analysis and wettability are studied by XPS and water contact angle (WCA), respectively. A lower monomer flow rate was found to result in a higher C-O/C-C ratio and a higher wettability of the deposited coatings.

Synthesis and Properties of Thiophene and Aniline Copolymer Using Atmospheric Pressure Plasma Jets Copolymerization Technique.

This paper investigates the properties of thiophene and aniline copolymer (TAC) films deposited by using atmospheric pressure plasma jets copolymerization technique relative to various blending ratios of aniline and thiophene monomer for synthesizing the donor-acceptor conjugated copolymers. Field emission scanning electron microscopy (FE-SEM) and atomic force microscopy are utilized to measure the surface morphology, roughness and film thickness of TAC films. Structural and chemical properties of TAC films are investigated by Fourier transforms-infrared spectroscopy (FT-IR), time of flight secondary ion mass spectrometry, and X-ray photoelectron spectroscopy. FE-SEM images show that the film thickness and nanoparticles size of the TAC films increase with an addition thiophene monomer in the aniline monomer. FE-SEM, FT-IR results show that TAC films are successfully synthesized on glass substrates in all cases. The iodine doped TAC film on the Si substrate with interdigitated electrodes shows the lowest electrical resistance at blending condition of thiophene of 25%.

Reaction kinetics of phenols and p-nitrophenols in flowing aerated aqueous solutions generated by a discharge plasma jet.

In this paper, we propose a method for removing phenols and p-nitrophenols (PNPs) from flowing aqueous solutions generated by atmospheric pressure plasma jets (APPJs). For analyzing the removal characteristics, multiple techniques were used, including flow speed analysis of the aerated solution, optical emission spectroscopy (OES), and liquid chromatography. In addition, the reaction kinetics of diffusion and activation control processes were evaluated using aerated fluid speed variation and the corresponding activation energy. From these results, the relative intensities of hydroxyl radicals produced by an APPJ in water were found to be stronger than those in air and to decrease with increasing flow speed. Furthermore, the reaction kinetics were found to be diffusion-controlled when the solution flow speed was low and activation-controlled under high solution flow speed. It was also found that the degradation efficiency was enhanced with increasing flow speed, which increased the discharge voltage and temperature of the solution and changed the initial pH value when TiO2/UV catalysis was used. From the complex relationship between the reactive species, fluid diffusion, and discharge parameters in wastewater described herein, it is anticipated that these findings will facilitate new approaches to both the design and optimization of discharge reactors intended for wastewater treatment.

Dual RGD-immobilized poly(L-lactic acid) by atmospheric pressure plasma jet for bone tissue engineering.

Surface treatment on PLA substrates by atmospheric pressure plasma jet (APPJ) for polymerization of dual RGD-peptides were investigated. Peptide-modified surfaces have been highlighted as the most promising approach to improve the integration of implants into surrounding bones. By varying the RF power, PLA substrates treated by APPJ process have a tendency to form a hydrophobic surface. The effects on the proliferation and differentiation of MG63 cells were evaluated and osteocalcin (OCN) expression was analyzed using RT-PCR. The water contact angle of the W/APPJ process PLA was approximately 54% of that of the W/O APPJ process PLA substrates. W/APPJ process significantly increased cell proliferation, improved the functionality of the material without using a complicated procedure. We believe that pretreatment using the APPJ processes and dual RGD grafting can be more appropriate than traditional surface modification methods, with more potential for application to bone materials.