The treatment of phosphogypsum leachate is more urgent than phosphogypsum.

Phosphogypsum(PG) is one of the typical bulk industrial solid wastes generated in the phosphate chemical industry. Due to its huge production volume and immature resource treatment technology, a large amount of PG can only be stored and disposed in slag yards, and its impact on the ecological environment is becoming increasingly significant during long-term storage. Up to now, many researchers have focused their research on PG, with less attention paid to the PG leachate(PG-L). On the basis of the resource utilization of PG, this article analyzed the migration and transformation of pollutants and their impact on the ecological environment during long-term storage of PG. The content of pollutants in PG-L and PG was compared, and it was found that the content of toxic and harmful substances in PG-L was significantly higher than that in PG itself, and the pollution diffusion ability was greater than that of PG, the pollution of PG to the ecological environment is mainly caused by PG-L, indicating that the harmless treatment of PG-L is more urgent than PG. On the basis of traditional leachate treatment methods, a new technology of valuable element recovery and electrochemical synergistic treatment is proposed to achieve high value-added treatment of PG-L.

Changes in the physicochemical properties and antioxidant capacity of Sichuan hotpot oil.

This study aimed to develop nutritious and healthy Sichuan hotpot oil. Four blended oil formulas were formulated using MATLAB based on the fatty acid composition of four base constituents (beef tallow, mutton tallow, peanut oil, and palm oil). The sensory characteristics, physicochemical properties, nutritional composition, harmful substances, and antioxidant capacity of the oils were analyzed during the boiling process. A blend of 60% beef tallow + 10% mutton tallow + 10% peanut oil + 20% palm oil exhibited a low level of peroxide (0.03 g/100 g) and malondialdehyde (0.04 mg/kg), and high phytosterol content (1028.33 mg/kg), which was the suitable hotpot blending oil. Furthermore, the changes in the physicochemical properties during boring were low, with a high retention rate of phytosterol (94.85%), and the levels of 3,4-benzopyrene (1.12 µg/kg) and 3-monochloropropane-1,2-diol ester (0.67 mg/kg) were both lower than the recommended limits. This study will provide a theoretical basis for the advancement of the hotpot oil industry.

Single-chain fragment variable produced by phage display technology: Construction, selection, mutation, expression, and recent applications in food safety.

Immunoassays are reliable, efficient, and accurate methods for the analysis of small-molecule harmful substances (such as pesticides, veterinary drugs, and biological toxins) that may be present in food. However, traditional polyclonal and monoclonal antibodies are limited by animal hosts and hinder further development of immunoassays. With the gradual application of phage display technology as an efficient in vitro selection technology, the single-chain fragment variable (scFv) now provides an exciting alternative to traditional antibodies. Efficiently constructed scFv source libraries and specifically designed biopanning schemes can now yield scFvs possessing specific recognition capabilities. A rational mutation strategy further enhances the affinity of scFv, and allows it to reach a level that cannot be achieved by immunization. Finally, appropriate prokaryotic expression measures ensure stable and efficient production of scFv. Therefore, when developing excellent scFvs, it is necessary to focus on three key aspects of this process that include screening, mutation, and expression. In this review, we analyze in detail the preparation and affinity improvement process for scFv and provide insights into the research progress and development trend of scFv-based immunoassay methods for monitoring small-molecule harmful substances.

Physicochemical properties of rice bran blended oil in deep frying by principal component analysis.

This study aimed to obtain a rice bran blended oil with good quality in deep frying. The thermal stability, nutrients and harmful substances of rice bran oil (RBO) and other four oils (palm oil, PO; cottonseed oil, CO; sunflower oil, SuO; soybean oil, SO) were analyzed. Besides, the blended oil formulas were established by the principal component analysis method, and their physicochemical properties, frying characteristic indicators, nutrients, and harmful substances were compared. The results provided that two suitable blended oil formulas (F1: 50% RBO + 40% PO + 10% CO; F2: 60% RBO + 35% PO + 5% CO) of good frying performance were attained by principal component analysis. The acid value (1.19 mg/g), peroxide value (0.09 meq/kg), residual oil rate (8.07%), 3-MCPD ester reduction content (2.33 mg/kg), benzopyrene concentration content (0.95 µg/kg) and vitamin E consumption rate (67.86%) in F2 were lower than that in F1. Moreover, the oryzanol retention rate (87.84%) of F2 was higher than that of F1. In summary, F2 was more conducive to human health and more suitable than F1 in deep-frying. This information had an important directive on the industrial production of rice bran blend oil. Supplementary Information: The online version contains supplementary material available at 10.1007/s13197-022-05472-7.

Allergens and food additives, including potentially harmful ones, present in food products that are preferred by children and adolescents.

INTRODUCTION: The proper development of a child is linked with proper nutrition, including nutritional habits which are formed from childhood. THE AIM OF THE STUDY: The aim of the study was to establish a list of the most popular food products among children and to develop a register of potentially dangerous substances on a Facebook website. MATERIALS AND METHODS: A website was created on Facebook. The participants provided lists of favorite dishes or products. RESULTS: The study involved 264 participants. An inverse correlation was observed with reference to the age of the subjects and the occurrence of sugar syrup in their diet (R=-0.20; p<0.001), glucose-fructose (R= -0.18; p< 0.004), and glucose (R=-0.13; p< 0.039) syrups. The most common potential food allergens are: gluten (R=0.28; p<0.001), eggs (R=0.28; p<0.001), and wheat (R=0.25; p<0.001). The main substances added to food that are present in a child's diet that increase proportionally with reference to the child's age are: salicylates (R=0.37; p<0.001), iron and ammonium sulfates (R=0.21; p<0.001). CONCLUSION: The choices of favorite products are related to age and sex. Products containing gluten, the consumption of which increases with age, carry a risk of undiagnosed celiac disease and non-celiac gluten sensitivity in people with a genetic predisposition. Facebook has fulfilled its role as an effective tool for gathering information about the food preferences of children and adolescents.

The formation and conversion of characteristic aroma profiles and key harmful substances in different high-temperature processing of hairtail (Trichiurus Haumela).

The balance regulation between characteristic aroma and hazards in high-temperature processed fish is a hot spot. This study was aimed to explore the interactive relationship between the nutritional value, microstructures, aroma, and harmful substances of hairtail under different frying methods including traditional frying (TF), air frying (AF), and vacuum frying (VF) via chemical pattern recognition. The results indicated that VF-prepared hairtail could form a crunchy mouthfeel and retain the highest content of protein (645.53 mg/g) and the lowest content of fat (242.03 mg/g). Vacuum frying reduced lipid oxidation in hairtail, resulting in the POV reaching 0.02 mg/g, significantly lower than that of TF (0.05 mg/g) and AF (0.21 mg/g), and TBARS reached 0.83 mg/g, significantly lower than that of AF (1.96 mg/g) (P < 0.05), respectively. Notable variations were observedin the aroma profileof hairtail preparedfrom different frying methods. Vacuum frying of hairtail resulted in higher levels of pyrazines and alcohols, whereas traditional frying and air frying were associated with the formation of aldehydes and ketones, respectively. Air frying was not a healthy way to cook hairtail which produced the highest concentration of harmful substances (up to 190.63 ng/g), significantly higher than VF (5.72 ng/g) and TF (52.78 ng/g) (P < 0.05), especially norharman (122.57 ng/g), significantly higher than VF (4.50 ng/g) and TF (32.63 ng/g) (P < 0.05). Norharman and acrylamide were the key harmful substances in hairtail treated with traditional frying. The vacuum frying method was an excellent alternative for deep-fried hairtail as a snack food with fewer harmful substances and a fine aroma, providing a theoretic guidance for preparing healthy hairtail food with high nutrition and superior sensory attraction.

Comparative Analysis of Frying Performance: Assessing Stability, Nutritional Value, and Safety of High-Oleic Rapeseed Oils.

Frying is a critical process in the food industry, where selecting appropriate vegetable oils is key to achieving optimal results. In this study, French fries were fried at 175 °C with five different oils, the changes in the physicochemical indexes and free radical scavenging rate of the oils during the frying process were investigated, and the most suitable oils for frying were identified through comparative analysis using principal component analysis (PCA). We assessed the frying performances of hot-pressed high-oleic-acid rapeseed oil (HHRO), cold-pressed high-oleic-acid rapeseed oil (CHRO), soybean oil, rice bran oil, and palm oil utilizing principal component analysis over an 18 h period. The HHRO and CHRO showed lower acid values (0.31, 0.26 mg/g), peroxide values (2.09, 1.96 g/100 g), p-anisidine values (152.48, 178.88 g/mL), and total polar compound percentages (27.60%, 32.10%) than other oils. Furthermore, both the HHRO and CHRO demonstrated enhanced free radical scavenging abilities, indicative of their higher antioxidant capacities, as corroborated by the PCA results. Benzopyridine, 3-monochloropropane-1,2-diol ester, squalene, tocopherols, and polyphenol from the HHRO and CHRO during frying were compared. A comprehensive examination of harmful substances versus nutrient retention during frying revealed that the HHRO contained fewer hazardous compounds, while CHRO retained more nutrients. Therefore, this study analyzes the oxidation regulation of HHRO in frying applications, highlights the prospects of HHRO for frying in terms of health and economy, and contributes valuable insights for informed vegetable oil selection within the food industry.

An analysis of exogenous harmful substance exposure as risk factors for COPD and hypertension co-morbidity using PSM.

Background: Some occupational and environmental exposures could increase the risk of chronic obstructive pulmonary disease (COPD) and hypertension in various work and living environments. However, the effect of exposure to multiple exogenous harmful substances on COPD and hypertension co-morbidities remains unclear. Methods: Participants were selected from eight hospitals in five provinces in China using a multistage cluster sampling procedure. Participants' demographic, exposure, and disease information were collected through questionnaires, spirometry, and blood pressure examinations. Demographic data were used as matching factors, and 1:1 matching between the exposed and non-exposed groups was performed by employing propensity score matching (PSM) to minimize the influence on the results. A one-way chi-squared analysis and multifactorial logistic regression were used to analyze the association between the exposure to exogenous harmful substances (metals and their compound dust, inorganic mineral dust, organic chemicals, and livestock by-products) and the co-morbidity of COPD and hypertension. Results: There were 6,610 eligible participants in the final analysis, of whom 2,045 (30.9%) were exposed to exogenous harmful substances. The prevalence of co-morbidities of COPD and hypertension (6.0%) in the exposure group was higher than their prevalence in the total population (4.6%). After PSM, exogenous harmful substance exposure was found to be a risk factor for the co-morbidity of COPD and hypertension [odds ratio (OR) = 1.347, 95% confidence interval (CI): 1.011-1.794], which was not statistically significant before PSM (OR = 1.094, 95% CI: 0.852-1.405). Meanwhile, the results of different outcomes showed that the association between hypertension and exogenous harmful substance exposure was not statistically significant (OR = 0.965, 95% CI: 0.846-1.101). Smoking (OR = 4.702, 95% CI: 3.321-6.656), history of a respiratory disease during childhood (OR = 2.830, 95% CI: 1.600-5.006), and history of respiratory symptoms (OR = 1.897, 95% CI: 1.331-2.704) were also identified as risk factors for the co-morbidity of COPD and hypertension. Conclusion: The distribution of exogenous harmful substance exposure varies in the population, and the prevalence of co-morbidities is generally higher in susceptible populations. Exposure to exogenous harmful substances was found to be a key risk factor after adjusting for demographic confounders.

Computer vision based deep learning approach for toxic and harmful substances detection in fruits.

Formaldehyde (CH2O) is one of the significant chemicals mixed with different perishable fruits in Bangladesh. The fruits are artificially preserved for extended periods by dishonest vendors using this dangerous chemical. Such substances are complicated to detect in appearance. Hence, a reliable and robust detection technique is required. To overcome this challenge and address the issue, we introduce comprehensive deep learning-based techniques for detecting toxic substances. Four different types of fruits, both in fresh and chemically mixed conditions, are used in this experiment. We have applied diverse data augmentation techniques to enlarge the dataset. The performance of four different pre-trained deep learning models was then assessed, and a brand-new model named "DurbeenNet," created especially for this task, was presented. The primary objective was to gauge the efficacy of our proposed model compared to well-established deep learning architectures. Our assessment centered on the models' accuracy in detecting toxic substances. According to our research, GoogleNet detected toxic substances with an accuracy rate of 85.53 %, VGG-16 with an accuracy rate of 87.44 %, DenseNet with an impressive accuracy rate of 90.37 %, and ResNet50 with an accuracy rate of 91.66 %. Notably, the proposed model, DurbeenNet, outshone all other models, boasting an impressive accuracy rate of 96.71 % in detecting toxic substances among the sample fruits.

Fast and sustainable planar yeast-based bioassay for endocrine disruptors in complex mixtures: Start of cell cultivation to result within one day.

High-performance thin-layer chromatography hyphenated with planar multiplex bioassays and high-resolution tandem mass spectrometry contributes to the non-target detection or even identification of active compounds in complex mixtures such as food, feed, cosmetics, commodities, and environmental samples. It can be used to discover previously unknown harmful or active substances in complex samples and to tentatively assign molecular formulas. This method is already faster than the commonly used in vitro assays along with liquid chromatographic separations, but overnight cell cultivation still prevents a planar bioassay from being performed within one day. There is also still potential for optimization in terms of sustainability. To achieve this, the planar bioassay protocols for the detection of androgen-like and estrogen-like compounds were harmonized. The successful minimization of the cell culture volume enabled accelerated cell cultivation, which allowed the bioassay to be performed within one day. This was considered a milestone achieved, as up to 23 samples per plate can now be analyzed from the start of cultivation to the biological endpoint on the same day. Doubling the substrate amount and increasing the pH of the silica gel layer led to a more sensitive and selective bioassay due to the enhanced fluorescence of the formed end-product. The faster and more sustainable bioassay protocol was applied to complex samples such as sunscreen and red wine to detect estrogen-like compounds. The developed method was validated by comparison with a standard method.

Optimization of Composite Decolorizer Efficacy Based on Decolorization Efficiency, Toxicity, and Nutritional Value of Rice Bran Oil.

This study compared the effect of five different adsorbents (activated clay, activated carbon, attapulgite clay, bentonite, diatomite) on the levels of nutrients, harmful substance retention, and decolorization in rice bran oil. Among the adsorbents tested, activated carbon displayed the highest decolorization efficiency (82.90%) and adsorption effect on 3,4-benzopyrene (BaP, 89.53%) and 3-monochloropropane-1,2-diol ester (41.55%), whereas activated clay had the highest oryzanol retention percentages (85.98%) and affordability. Activated carbon and activated clay were therefore selected as composite decolorizing agents. Based on single-factor and Box-Behnken response surface tests, the optimal conditions for decolorization efficiency (97.08%), oryzanol retention (89.62%), sterol retention (90.16%), vitamin E retention (79.91%), and benzo(a)pyrene adsorption percentages (95.98%) were determined to be achieved by using a 5% (w/w) composite decolorant (activated clay:activated carbon=5:1), at a temperature of 116℃, with an incubation time of 33 min. This study provides evidence to support the efficacy of compound decolorants, which may have practical use in large-scale industrial applications of edible oil decolorization during refinement.

Prenatal harmful substances: Thai pregnant women's experiences.

Background: The exposure of unborn babies to harmful substances during prenatal stages can lead to fetal anomalies, emphasizing the significance of pregnant women's practices in ensuring optimal fetal outcomes. However, there is a lack of understanding regarding this issue from the perspective of pregnant women. Objective: This study aimed to describe the experiences of pregnant women in avoiding harmful substances to their unborn babies. Methods: This study used a qualitative descriptive approach. Data were collected between September 2020 and April 2021 through in-depth interviews with 17 pregnant women purposively selected from two hospitals in southern Thailand. Data were transcribed verbatim and analyzed using thematic analysis. Results: Four themes emerged from the participants' experiences: 1) understanding of harms, which included substance characteristics, exposure characteristics, body's protective mechanism, personal experiences, and obtained information; 2) practices for safety, which consisted of food safety, work safety, ambient air safety, and safety in personal/household products; 3) challenges in avoiding harmful substances, which were unrecognition, unavailability of safe substances, discomfort and inconvenience, inevitability, and family traditions; and 4) overcoming the challenges through personal changes, obtaining support, and ensuring a peaceful mind. Conclusion: The findings provide valuable insights into prenatal exposure to harmful substances, which can serve as a basis for developing comprehensive guidelines for best practices. It is crucial for nurses to receive training that enables them to educate pregnant women, enhancing their awareness of important harmful substances and promoting effective methods for maintaining safety in their daily activities. Additionally, nurses can develop interventions to empower pregnant women to overcome challenges by involving significant individuals, particularly family members, in providing support and creating an optimal prenatal environment.

Novel Water-Oil Mixed Frying: Fried Oil Quality and the Formation of Heterocyclic Amines and Trans Fatty Acids in Fried Duck.

The present study was conducted to explore the impact of novel water-oil mixed frying and traditional oil frying methods on the soybean oil quality and formation of trans fatty acids (TFAs) and heterocyclic amines (HCAs) in fried duck breast and skin during 60 frying cycles. The acid value of the soybean oil was 2.10 mg/g using the traditional oil frying and 1.08 mg/g using water-oil mixed frying at the 60th frying cycle. The peroxide value of the water-oil mixed fried soybean oil was significantly lower than that of the traditional frying method. Water-oil mixed frying delayed the formation of TFAs in the soybean oil. The traditionally oil fried skin showed increased TFAs (9tC16:1) content from 0.17 to 0.22 mg/g (29.4% increase), while those of the water-oil mixed fried samples increased from 0.16 to 0.20 mg/g (25.00% increase) compared to control. Harman, Norharman and AαC were detected in all the fried samples and PhIP was observed after the 20th frying cycle, while MeAαC was formed only at the 60th frying cycle in traditional fried duck breast. Therefore, it is concluded that the water-oil mixed frying method is more suitable to improve the quality and safety of fried duck skin and breast meat.

Fire retardant performance, toxicity and combustion characteristics, and numerical evaluation of core materials for sandwich panels.

According to fire accident statistics, fires in buildings are increasing. The flame-retardant performance of insulation materials is considered an important factor for preventing the spread of fire and ensuring evacuation. This study evaluated the flame-retardant performance and combustion characteristics of four types of organic thermal insulation used as core materials in sandwich panels. The flame-retardant performance evaluation based on total heat release and heat release rate revealed that phenolic foam (PF) satisfied the criteria for non-combustible grade insulation. An analysis of the hazardous gases released while combustion of the four insulation materials indicated that a significant amount of CO was released-an average of 19,000 ppm or higher-in the rigid urethan foam (PIR) and spray-type polyurethane foam (SPU). The fractional effective dose (FED) value was derived from the gas analysis results according to ISO 13344. PIR and SPU had an average FED value of 2.0 or higher and were identified as very dangerous in the case of fire accidents. Moreover, the evacuation time in the case of a fire in a warehouse-type building was comprehensively analyzed considering the material, size, and height for the four types of insulation. PIR was the most vulnerable to fire, and for PF, the danger limit was not reached until the end of the simulation.

Alcohol, Tobacco and Cannabis Consumption on Physical Activity and Physical and Social Self-Concept in Secondary School Students: An Explanatory Model Regarding Gender.

Nowadays, the adolescent population consumes substances that are harmful to health at an earlier age. Therefore, the present research aimed to (i) develop an explanatory model of tobacco, alcohol, and cannabis consumption on physical self-concept, social self-concept, and physical activity practice and (ii) contrast the model through a multi-group analysis according to the gender of the participants. For this purpose, descriptive, comparative, and cross-sectional research was carried out on adolescent students (M = 13.91; SD = 1.31, years), using the Self-Concept Form 5 Questionnaire, the Physical Activity Questionnaire for Adolescents (PAQ-A), and the State Survey on Drug Use in Secondary Education (ETUDES) for data collection. Findings revealed that the consumption of harmful substances has a positive impact on the social area and the practice of physical exercise, showing a negative relationship between the latter variable and the social and physical area of self-concept.

Effects of Deodorization on the Formation of Processing Contaminants and Chemical Quality of Sunflower Oil.

Tocopherols and phytosterols are generally considered to be nutritionally beneficial, and 3-Monochloropropane-1,2-diol esters (3-MCPD esters), glycidyl esters (GEs) and trans fatty acids (TFAs) are generally considered to be harmful. The high temperature deodorization step is when these harmful 3-MCPD esters, GEs and TFAs are generated. Knowing how deodorization conditions affect levels of these substances is essential for designing refining processes that will produce nutritious, high quality edible oils. This study analyzed the changes of these components of sunflower oil at different temperatures (210, 230, 250 and 270°C) and times (60, 80, 100 and 120 min) during deodorization. Our research found that during the whole deodorization process (including undeodorized sunflower oil), the contents of 3-MCPD esters, GEs and TFAs all progressively increased, from 0.47 to 11.18 mg/kg, 0.24 to 18.42 mg/kg and 0.062% to 0.698%, respectively. However, the deodorization process significantly decreased the levels of tocopherols (from 535.94 to 240.26 mg/kg) and phytosterols (from 2803.58 to 1864.34 mg/kg). Meanwhile, the retention ratios of total tocopherols and total phytosterols also decreased from 96.29% to 44.83% and 92.29% to 66.50%, respectively.

Study of Psychosocial and Academic Indicators in Young Adults from Andalucía, Spain.

BACKGROUND: The university years bring a great deal of vital changes. In addition, psycho-social factors play a key role in university students' development and their consumption of harmful substances. The aim is to analyse academic performance according to psychosocial factors, self-concept, emotional intelligence and harmful substance consumption in a sample of future Physical Education teachers. METHODS: The present study was carried out with a sample of 775 undergraduate students who were studying for the Primary Education Degree in Physical Education in Andalusia, Spain. The main instruments used include an ad hoc questionnaire, the Self-Concept Form-5 test, Alcohol Use Disorders Identification Test, Fagerström Test for Nicotine Dependence and the Emotional Intelligence Inventory adapted to Sport. RESULTS: The results show that young people who have a greater general self-concept and self-emotional management are those who access the university degree through Vocational Training. In relation to the average record mark, it was observed that participants with marks of notable and outstanding obtained higher scores in general self-concept, academic dimension and hetero-emotional management. Those with grades of passed and outstanding were those with higher levels in the physical dimension of self-concept and emotional use. In relation to receiving a scholarship to study, it has been shown that those university students who do perceive it have higher levels in most of the dimensions of emotional intelligence and self-concept. CONCLUSIONS: Finally, the harmful substances did not show significant relationships with academic factors, except between tobacco and the average mark of the file.

Risk assessment and analysis of harmful residues in edible agricultural products in China-take Anhui Province as an example.

The reforming and opening-up and the development of agricultural science and technology have brought about the rapid growth of China's food quantity and solved the problem that 20% of the world's population has enough to eat. At the same time, it also brings the problem of harmful residues in food. On the one hand, the Chinese government guides farmers to rationally use chemical fertilizers, pesticides, and veterinary drugs. On the other hand, in urban and rural school canteens and farmers' markets in third-tier cities, the implementation of pesticide residue detection system. Through the investigation of 24 kinds of edible agricultural products which are easy to produce harmful residues, and using a variety of statistical methods, the results showed that at present, the residues of harmful substances in edible agricultural products consumed by urban residents and school canteens in urban and rural areas have been well controlled, and the compliance rate is about 80%. The residues of harmful substances in edible agricultural products consumed by rural residents obviously exceed the standard. Taking rice production as an example, although farmers can reduce the risk of excessive pesticide residues in rice through rational application of pesticides, they are more worried about less application of pesticides and bear the risk of reducing rice production. Most farmers still choose to take the risk of excessive pesticide residues.

Advanced converter sludge utilization technologies for the recovery of valuable elements: A review.

Due to the high proportion of the steel output produced by oxygen converter, significant quantities of converter sludge (CS) is generated annually as waste material. This study aims to review the latest CS utilization technologies and illuminate the migration behaviors of harmful substances as well as valuable elements. The intrinsic characteristics, including chemical constitution, size distribution, mineralogical composition, microstructure, and viscosity of the CS are studied. Migration behaviors of harmful substances are analyzed based on thermodynamic calculation. The results indicated that less eutectic mineral was found in CS, the iron oxides and other impurities like CaO, MgO and ZnFe2O4 mixed in the way of physical accumulation. The treatments through oxidation methods, such as iron ore sintering and oxidized pellets, are the most common and effective methods to recovery Fe in actual production. Due to the diverse physicochemical properties of CS from different enterprises, it is really difficult to choose one universal recovery method. In view of resources recovery and clean production, the authors believe that the best utilization technology at present is to prepare metallized pellets. It is regarded that technologies of preparing high value-added products, such as Li(FeM)PO4 and iron powder are the most prospective methods in the future.

Evaluation and Behavior of Spanish Bodybuilders: Doping and Sports Supplements.

The use of doping agents has these days become a public health problem, as it also affects young and non-competitive amateurs in different sports. To prepare for competition, bodybuilders perform aggressive dietary protocols, so, bodybuilders frequently consume nutritional supplements (NS) and banned substances in large dosages. Thus, the aim of this study is to analyze the prevalence of banned substances consumption and NS intake in competitive level bodybuilders. A total of 48 bodybuilders (44 males and 4 females) completed a validated online questionnaire on NS consumption. The quantitative data was presented as a mean (M) ± standard deviation (SD), as well as having minimum and maximum values. The categorical variables were expressed using frequencies and percentages. 83.3% of the participants declared that they had consumed or would consume banned substances, the most consumed being anabolic steroids (72.9%). One hundred percent of those sampled use NS. Whey protein (96%), branched-chain amino acids (BCAA) (94%), creatine (85%) and vitamin complexes (83%) were the most consumed, however, there is a low consumption of certain NS which could also increase athletic performance.