Estimating age-at-death in burnt adult human remains using the Falys-Prangle method.

OBJECTIVES: The Falys-Prangle-method assesses age-related morphological changes to the sternal clavicle end (SCE), enabling the observation of mature adults from the 5th decade onwards in unburnt human skeletal remains. The aim of this study is to investigate the applicability of the Falys-Prangle-method on burnt human remains. MATERIALS AND METHODS: Fifty-two SCE of 40 cremated individuals (out of 86) from the William M. Bass collection of the Forensic Anthropology Center (Knoxville, Tennessee) of known age-at-death and sex are available for assessment. Surface topography, porosity, and osteophyte formation are evaluated, after which the calculated composite score is associated with the corresponding age range as described by Falys and Prangle. The method is also applied on an archaeological case study from Oudenburg, Belgium, dating to the Roman period. RESULTS: The assessed age ranges strongly agree with the true age ranges (α = 0.828), suggesting the Falys-Prangle-method to be applicable on burnt human remains. The case study from Oudenburg yields markedly improved age-at-death estimates, significantly enhancing our understanding of the age distribution within this community. DISCUSSION: Information on age-at-death is key in the construction of biological profiles of past individuals. The mature adult is often invisible in the archaeological record since most macroscopic age estimation methods do not distinguish beyond 46+ years old. Our study stresses the usefulness of a large-scale application of the Falys-Prangle-method, which will increase the visibility of mature adults, especially in archaeological burnt human skeletal collections, where such information is, at present, extremely difficult to obtain.

Determination of age-specific reference intervals for selected serum and urinary biomarkers in elderly cats.

OBJECTIVES: Annual health screening is recommended in elderly cats to allow the early detection of conditions such as chronic kidney disease (CKD) and hyperthyroidism. Nevertheless, age-specific reference intervals (RIs) for renal and thyroid parameters in this population are lacking. The aim of this study was to determine age-specific RIs for selected serum and urine biomarkers related to CKD and hyperthyroidism, namely serum creatinine (sCr), symmetric dimethylarginine (SDMA), phosphate (P), total calcium (tCa), total thyroxine (TT4), urinary protein:creatinine ratio (UPC) and urine specific gravity (USG). These RIs were established for elderly cats (aged ⩾7 years) in general, as well as for mature adult cats (aged 7-10 years) and senior cats (aged >10 years) separately. METHODS: A prospective study was conducted on client-owned cats aged ⩾7 years and considered healthy by their owners. The cats were screened to rule out metabolic and systemic diseases by means of a thorough history, complete physical examination, blood examination and urinalysis. The data from 206 healthy elderly cats (134 mature adult and 72 senior cats) were included. Age-appropriate RIs were determined following the guidelines of the American Society of Veterinary Clinical Pathology and compared with existing laboratory RIs. RESULTS: Clinically relevant differences between the age-specific RI and the laboratory RI were found for several variables. Compared with the laboratory RI, the upper limit of the RI for cats aged ⩾7 years was lower for sCr, TT4 and P, and higher for SDMA. The lower limit of the age-appropriate RI was lower for USG. The new RI was almost identical to the existing laboratory RI for tCa and UPC. CONCLUSIONS AND RELEVANCE: Using age-specific RIs for renal and thyroid biomarkers in mature adult and senior cats has important clinical consequences for the interpretation of health screening results in elderly cats. This confirms the need to adapt laboratory RIs to the specific animal population for which the RI will be used.

Dataset on transcriptome signature of skeletal muscle of young, adult and aged mice.

This data article reports the level of expression of messenger RNA (mRNA) obtained from a set of 18 skeletal muscle samples using Affymetrix Genechips Exon arrays. Data were obtained from Gastrocnemius muscle of C57BL6 male mice at 3 distinct age groups, 2, 11 and 25 months old representing young, mature adult and aged groups. The data submitted to GEO constitute a large dataset of 15,300 mRNA levels. The data include eighteen .CEL files obtained after scanning mouse exon arrays and one .xls file obtained after processing with Genespring GX 14.9. Three distinct files containing affymetrix data processed using Genespring and analyzed for differences between stages 2 per 2 are provided as supplementary data.

Activation of 6-8-week-old new mature adult-born dentate granule cells contributes to anxiety-like behavior.

Adult-born dentate granule cells (aDGCs) at 4-6 weeks of age are particularly excitable but subsequently develop the quiet properties of mature cells. Most existing studies have focused on the hyperactivity of 4-6-week-old aDGCs or neurogenesis, which confers stress resilience or buffers stress responses. However, the function of the quiet property of new mature aDGCs remains unclear. Here we used a retrovirus expressing cre recombinase in combination with an associated-adenovirus to specifically interfere with the activity of new mature aDGCs, and estimated anxiety-like behaviors by the open-field test and elevated plus maze test, antidepressant-like behaviors by the tail suspension test, and spatial memory by the Barnes maze test. We found that sustained hyperactivity of 6-8-week-old, but not 8-10-week-old, aDGCs induced anxiety-like behaviors, and suppression of the activity of 6-8-week-old aDGCs disturbed spatial memory. Meanwhile, sustained hyperactivity of 6-8-week-old aDGCs induced activation of mature dentate gyrus (DG) neurons and inhibition of immature aDGCs. Additionally, the mice showing anxiety-like behaviors induced by chronic mild immobilization stress exhibited increased activity in 6-8-week-old aDGCs. Furthermore, the sustained hyperactivity of mature DG neurons also induced anxiety-like behaviors and decreased the activity of immature aDGCs. Our results combined show that the excitation of 6-8-week-old new mature aDGCs, which prohibits them from normally entering the resting state, determines anxiety-like behavior, while the maintenance of normal excitation ability of 6-8-week-old new mature aDGCs confers memory. Our results suggests that strategies aimed at inhibiting unusual hyperactive new mature aDGCs at a restricted time window may protect against stress-related psychiatric disorders, such as anxiety and depression.