The dimensionality of infection networks among viruses infecting microbial eukaryotes and bacteria.

Diverse viruses and their hosts are interconnected through complex networks of infection, which are thought to influence ecological and evolutionary processes, but the principles underlying infection network structure are not well understood. Here we focus on network dimensionality and how it varies across 37 networks of viruses infecting eukaryotic phytoplankton and bacteria. We find that dimensionality is often strikingly low, with most networks being one- or two-dimensional, although dimensionality increases with network richness, suggesting that the true dimensionality of natural systems is higher. Low-dimensional networks generally exhibit a mixture of host partitioning among viruses and nestededness of host ranges. Networks of bacteria-infecting and eukaryote-infecting viruses possess comparable distributions of dimensionality and prevalence of nestedness, indicating that fundamentals of network structure are similar among domains of life and different viral lineages. The relative simplicity of many infection networks suggests that coevolutionary dynamics are often driven by a modest number of underlying mechanisms.

Resource partitioning in a novel herbivore assemblage in South America.

Human-induced species declines and extinctions have led to the downsizing of large-herbivore assemblages, with implications for many ecosystem processes. Active reintroduction of extirpated large herbivores or their functional equivalents may help to reverse this trend and restore diverse ecosystems and their processes. However, it is unclear whether resource competition between native and non-native herbivores could threaten restoration initiatives, or to what extent (re)introduced species may influence local vegetation dynamics. To answer these questions, we investigated the diets of a novel South American herbivore assemblage that includes resident native species, reintroduced native species and introduced non-native species. We examined plant composition, diet breadth and the overlap between species to describe the local herbivory profile and the potential for resource competition. Using DNA metabarcoding on faecal samples (n = 465), we analysed the diets of the herbivore assemblage in the Rincón del Socorro rewilding area of Iberá National Park, Argentina. We compared the species richness of faecal samples, the occurrence of plant families/growth forms and the compositional similarity of samples (inter- and intraspecifically). Our results indicate species-level taxonomic partitioning of plant resources by herbivores in this system. Differences in sample richness, composition and diet breadth reflected a diverse range of herbivory strategies, from grazers (capybara) to mixed feeders/browsers (brocket deer, lowland tapir). Differences in diet compositional similarity (Jaccard) revealed strong taxonomic resource partitioning. The two herbivores with the most similar diets (Pampas deer and brocket deer) still differed by more than 80%. Furthermore, all but one species (axis deer) had more similar diet composition intraspecifically than compared to the others. Overall, we found little evidence for resource competition between herbivore species. Instead, recently reintroduced native species and historically introduced non-natives are likely expanding the range of herbivory dynamics in the ecosystem. Further research will be needed to determine the full ecological impacts of these (re)introduced herbivores. In conclusion, we show clear differences in diet breadth and composition among native, reintroduced and non-native herbivore species that may be key to promoting resource partitioning, species coexistence and the restoration of ecological function.

La disminución y extinción de especies ocasionada por el hombre ha llevado a la reducción de tamaño de las comunidades de grandes herbívoros, con implicaciones para muchos procesos ecosistémicos. La reintroducción activa de grandes herbívoros extirpados, o sus equivalentes funcionales, puede ayudar a revertir esta tendencia y restaurar diversos ecosistemas y sus procesos. Sin embargo, no está claro si la competencia por recursos entre herbívoros nativos y no nativos podría amenazar las iniciativas de restauración, o en qué medida las especies (re)introducidas pueden influir la dinámica de la vegetación local. Para responder a estas preguntas, investigamos las dietas de una comunidad de herbívoros sudamericanos que incluye especies nativas, especies nativas reintroducidas y especies no nativas introducidas. Examinamos la composición de plantas, la amplitud de la dieta y la superposición entre especies para describir el perfil herbívoro local y el potencial de competencia por los recursos. Utilizando metabarcoding de ADN en muestras fecales (n = 465), analizamos las dietas de la comunidad de herbívoros en el sitio de rewilding Rincón del Socorro dentro del Parque Nacional Iberá, Argentina. Comparamos la riqueza de especies en las muestras fecales, la ocurrencia de familias de plantas/formas de crecimiento y la similitud en la composición de las muestras (interespecíficamente e intraespecíficamente). Nuestros resultados indican la partición taxonómica a nivel de especie de los recursos vegetales por parte de los herbívoros en este sistema. Las diferencias en la riqueza de las muestras, la composición y la amplitud de las dietas reflejaron una amplia gama de estrategias de herbivoría, desde pastoreadores (capibara) hasta herbívoros mixtos/ramoneadores (corzuela, tapir amazónico). Las diferencias en la similitud de la composición de la dieta (Jaccard) revelaron una fuerte partición taxonómica de los recursos. Los dos herbívoros con las dietas más similares (venado de las pampas y corzuela), aún así diferían en más del 80%. Además, todas las especies menos una (ciervo axis) tenían una composición dietética más similar intraespecíficamente que en comparación con las demás. En general, encontramos poca evidencia de competencia por recursos entre las especies de herbívoros. En cambio, las especies nativas reintroducidas recientemente y las no nativas introducidas históricamente probablemente estén ampliando el rango de dinámica de herbivoría en el ecosistema. Se necesitarán más investigaciones para determinar todos los impactos ecológicos de estos herbívoros (re)introducidos. En conclusión, mostramos diferencias claras en la amplitud y composición de la dieta entre especies de herbívoros nativas, reintroducidas y no nativas que pueden ser clave para promover la partición de recursos, la coexistencia de especies y la restauración de las funciones ecológicas.

Effect of uneven tolerance to human disturbance on dominance interactions of top predators.

Anthropogenic activities may alter felid assemblage structure, facilitating the persistence of tolerant species (commonly mesopredators), excluding ecologically demanding ones (top predators) and, consequently, changing coexistence rules. We aimed to determine how human activities influence intraguild relationships among top predators and their cascading effects on mesopredators, which remain poorly understood despite evidence of top carnivore decline. We used structural equation modeling at a continental scale to investigate how habitat quality and quantity, livestock density, and other human pressures modified the intraguild relations of the 3 species that are at the top of the food chain in the Neotropics: jaguars (Panthera onca), pumas (Puma concolor), and ocelots (Leopardus pardalis). We included presence-absence data derived from systematic studies compiled in Neocarnivores data set for these felid species at 0.0833° resolution. Human disturbance reduced the probability of jaguar occurrence by -0.35 standard deviations. Unexpectedly, the presence of sheep (Ovis aries) or goats (Capra aegagrus hircus) and jaguars was positively related to the presence of pumas, whereas puma presence was negatively related to the presence of ocelots. Extent of forest cover had more of an effect on jaguar (ß = 0.23) and ocelot (ß = 0.12) occurrences than the extent of protected area, which did not have a significant effect. The lack of effect of human activities on puma presence and the positive effect of small livestock supports the notion that pumas are more adaptable to habitat disturbance than jaguars. Our findings suggest that human disturbance has the potential to reverse the hierarchical competition dominance among large felids, leading to an unbalanced community structure. This shift disadvantages jaguars and elevates the position of pumas in the assemblage hierarchy, resulting in the exclusion of ocelots, despite their relatively lower susceptibility to anthropogenic disturbance. Our results suggest that conservation efforts should extend beyond protected areas to encompass the surrounding landscape, where complexities and potential conflicts are more pronounced.

Efectos de la tolerancia dispareja a las perturbaciones humanas sobre las interacciones de dominancia de los depredadores mayores Resumen Las actividades antropogénicas pueden alterar la estructura de las poblaciones de félidos, lo que facilita la persistencia de especies tolerantes (normalmente mesodepredadores), excluye a otras especies con exigencias ecológicas (depredadores mayores) y, en consecuencia, modifica las reglas de coexistencia. Buscamos determinar cómo influyen las actividades humanas sobre las relaciones entre gremios de los depredadores superiores y sus efectos en cascada sobre los mesodepredadores, que aún son poco conocidos a pesar de las pruebas del declive de los carnívoros superiores. Utilizamos modelos de ecuaciones estructurales a escala continental para investigar cómo la calidad y cantidad del hábitat, la densidad ganadera y otras presiones humanas modifican las relaciones entre gremios de las tres especies que se encuentran en la cima de la cadena trófica en el Neotrópico: jaguares (Panthera onca), pumas (Puma concolor) y ocelotes (Leopardus pardalis). Incluimos datos de presencia­ausencia derivados de estudios sistemáticos recopilados en el conjunto de datos Neocarnivores para estas especies de félidos con una resolución de 0.0833°. Las perturbaciones humanas redujeron la probabilidad de aparición del jaguar en ­0.35 desviaciones estándar. De forma inesperada, la presencia de ovejas (Ovis aries) o cabras (Capra aegargrus hircus) y jaguares tuvo una relación positiva con la presencia de pumas, mientras que la presencia de pumas tuvo una relación negativa con la presencia de ocelotes. La extensión de la cubierta forestal tuvo más efecto sobre la presencia de jaguares (ß = 0.23) y ocelotes (ß = 0.12) que la extensión del área protegida, que no tuvo un efecto significativo. La falta de efectos de las actividades humanas sobre la presencia del puma y el efecto positivo del ganado menor apoyan la idea de que los pumas son más adaptables a las perturbaciones del hábitat que los jaguares. Nuestros hallazgos sugieren que las perturbaciones humanas tienen el potencial de invertir la dominancia jerárquica de competencia entre los grandes félidos, lo que lleva a una estructura comunitaria desequilibrada. Este cambio perjudica a los jaguares y eleva la posición de los pumas en la jerarquía del ensamblaje, lo que excluye a los ocelotes, a pesar de su relativamente menor susceptibilidad a las perturbaciones antropogénicas. Nuestros resultados sugieren que los esfuerzos de conservación deberían extenderse más allá de las áreas protegidas para abarcar el paisaje circundante, donde las complejidades y los conflictos potenciales son más pronunciados.

Resource use differences of two coexisting chironomid species at localized scales.

Competing species may show positive correlations in abundance through time and space if they rely on a shared resource. Such positive correlations might obscure resource partitioning that facilitates competitor coexistence. Here, we examine the potential for resource partitioning between two ecologically similar midge species (Diptera: Chironomidae) in Lake Mývatn, Iceland. Tanytarsus gracilentus and Chironomus islandicus show large, roughly synchronized population fluctuations, implying potential reliance on a shared fluctuating resource and thereby posing the question of how these species coexist at high larval abundances. We first considered spatial partitioning of larvae. Abundances of both species were positively correlated in space; thus, spatial partitioning across different sites in the lake did not appear to be strong. We then inferred differences in dietary resources with stable carbon isotopes. T. gracilentus larvae had significantly higher δ13C values than C. islandicus, suggesting interspecific differences in resource use. Differences in resource selectivity, tube-building behavior, and feeding styles may facilitate resource partitioning between these species. Relative to surface sediments, T. gracilentus had higher δ13C values, suggesting that they selectively graze on 13C-enriched resources such as productive algae from the surface of their tubes. In contrast, C. islandicus had lower δ13C values than surface sediments, suggesting reliance on 13C-depleted resources that may include detrital organic matter and associated microbes that larvae selectively consume from the sediment surface or within their burrow walls. Overall, our study illustrates that coexisting and ecologically similar species may show positive correlations in space and time while using different resources at fine spatial scales.

Divide and conquer: Spatial and temporal resource partitioning structures benthic cyanobacterial mats.

Benthic cyanobacterial mats are increasing in abundance worldwide with the potential to degrade ecosystem structure and function. Understanding mat community dynamics is thus critical for predicting mat growth and proliferation and for mitigating any associated negative effects. Carbon, nitrogen, and sulfur cycling are the predominant forms of nutrient cycling discussed within the literature, while metabolic cooperation and viral interactions are understudied. Although many forms of nutrient cycling in mats have been assessed, the links between niche dynamics, microbial interactions, and nutrient cycling are not well described. Here, we present an updated review on how nutrient cycling and microbial community interactions in mats are structured by resource partitioning via spatial and temporal heterogeneity and succession. We assess community interactions and nutrient cycling at both intramat and metacommunity scales. Additionally, we present ideas and recommendations for research in this area, highlighting top-down control, boundary layers, and metabolic cooperation as important future directions.

Phytoplankton exudates and lysates support distinct microbial consortia with specialized metabolic and ecophysiological traits.

Blooms of marine phytoplankton fix complex pools of dissolved organic matter (DOM) that are thought to be partitioned among hundreds of heterotrophic microbes at the base of the food web. While the relationship between microbial consumers and phytoplankton DOM is a key component of marine carbon cycling, microbial loop metabolism is largely understood from model organisms and substrates. Here, we took an untargeted approach to measure and analyze partitioning of four distinct phytoplankton-derived DOM pools among heterotrophic populations in a natural microbial community using a combination of ecogenomics, stable isotope probing (SIP), and proteomics. Each 13C-labeled exudate or lysate from a diatom or a picocyanobacterium was preferentially assimilated by different heterotrophic taxa with specialized metabolic and physiological adaptations. Bacteroidetes populations, with their unique high-molecular-weight transporters, were superior competitors for DOM derived from diatom cell lysis, rapidly increasing growth rates and ribosomal protein expression to produce new relatively high C:N biomass. Proteobacteria responses varied, with relatively low levels of assimilation by Gammaproteobacteria populations, while copiotrophic Alphaproteobacteria such as the Roseobacter clade, with their diverse array of ABC- and TRAP-type transporters to scavenge monomers and nitrogen-rich metabolites, accounted for nearly all cyanobacteria exudate assimilation and produced new relatively low C:N biomass. Carbon assimilation rates calculated from SIP data show that exudate and lysate from two common marine phytoplankton are being used by taxonomically distinct sets of heterotrophic populations with unique metabolic adaptations, providing a deeper mechanistic understanding of consumer succession and carbon use during marine bloom events.

Coexistence, resource partitioning, and fisheries management: A tale of two mesopredators in equatorial waters.

Rock hind (Epinephelus adscensionis) and spotted moray (Gymnothorax moringa) are ubiquitous mesopredators that co-occur in the nearshore waters of Ascension Island in the South Atlantic Ocean, where they have significant cultural and subsistence value, but management of their non-commercial take is limited. This isolated volcanic system is home to high biomass and low species diversity, which poses two key questions: How can two mesopredators that perform similar ecological roles coexist? And if these two species are so ecologically similar, can they be managed using the same approach? Here, we combined acoustic telemetry, stomach content analysis, and stable isotope analysis to (i) explore space use and diet choices within and between these two species and (ii) to assess appropriate species-specific management options. Although rock hind had high residency and small calculated home ranges (0.0001-0.3114 km2), spotted moray exhibited shorter periods of residency (<3 months) before exiting the array. Vertical space use differed significantly across the 20-month tracking period, with individual differences in vertical space observed for both species. A hierarchical generalized additive model using 12-h averaged depth data identified that rock hind occurred lower in the water column than spotted moray, with both species occupying moderately deeper depths at night versus day (+1.6% relative depth). Spotted moray depth was also significantly predicted by lunar illumination. Aggregating samples by species and tissue type, Bayesian ecological niche modeling identified a 53.14%-54.15% and 78.02%-97.08% probability of niche overlap from fin clip and white muscle, respectively, whereas limited stomach content data indicated a preference for piscivorous prey. Variability in niche breadth between years suggests these species may exploit a range of prey items over time. These findings indicate that although these two species perform a similar ecological role by feeding on prey occupying the same trophic levels, subtle differences in movement behaviors between them suggest a one-rule-fits-all management approach is not likely the most effective option.

On the De Novo Emergence of Ecological Interactions during Evolutionary Diversification: A Conceptual Framework and Experimental Test.

AbstractEcological interactions are crucial to the structure and function of biological communities, but we lack a causal understanding of the forces shaping their emergence during evolutionary diversification. Here we provide a conceptual framework linking different modes of diversification (e.g., ecological diversification), which depend on environmental characteristics, to the evolution of different forms of ecological interactions (e.g., resource partitioning) in asexual lineages. We tested the framework by examining the net interactions in communities of Pseudomonas aeruginosa produced via experimental evolution in nutritionally simple (SIM) or complex (COM) environments by contrasting the productivity and competitive fitness of whole evolved communities relative to their component isolates. As expected, we found that nutritional complexity drove the evolution of communities with net positive interactions whereas SIM communities had similar performance as their component isolates. A follow-up experiment revealed that high fitness in two COM communities was driven by rare variants (frequency <0.1%) that antagonized PA14, the ancestral strain and common competitor used in fitness assays. Our study suggests that the evolution of de novo ecological interactions in asexual lineages is predictable at a broad scale from environmental conditions. Further, our work demonstrates that rare variants can disproportionately impact the function of relatively simple microbial communities.

Intraguild predation is increased in areas of low prey diversity in a generalist predator community.

Niche differentiation and intraguild predation (IGP) can allow ecologically similar species to coexist, although it is unclear which coexistence mechanism predominates in consumer communities. Until now, a limited ability to quantify diets from metabarcoding data has precluded the use of sequencing data to determine the relative importance of these mechanisms. Here, we pair a recent metabarcoding quantification approach with stable isotope analysis to examine diet composition in a wolf spider community. We compare the prevalence of resource partitioning and IGP in these spiders and test whether factors that influence foraging performance, including individual identity, morphology, prey community and environmental conditions, can explain variation in diet composition and IGP. Extensive IGP is likely the primary coexistence mechanism in this community, and other factors to which foraging variation is often attributed do not explain diet composition and IGP here. Rather, IGP increases as prey diversity decreases. Foragers are driven to IGP where resource niches are limited. We highlight the need to examine how drivers of predator-prey interaction strengths translate into foraging in natural systems.

Fungal feeding preferences and molecular gut content analysis of two abundant oribatid mite species (Acari: Oribatida) under the canopy of Prosopis laevigata (Fabaceae) in a semi-arid land.

Prosopis laevigata (mesquite; Fabaceae) forms fertility islands in soils of semi-arid lands where microbial diversity concentrates in response to the accumulation of resources in the soil beneath individual plants, promoting organic matter decomposition and nutrient cycling. This phenomenon provides suitable conditions for the proliferation of key edaphic elements such as fungi and mites. Mite-fungal interactions are central for our understanding of nutrient cycling processes in resource-limited arid food webs; yet, no information is available about fertility islands in semi-arid lands. Thus, we aimed to determine in vitro fungal-based feeding preferences and molecular gut content of the oribatid mite species Zygoribatula cf. floridana and Scheloribates cf. laevigatus, which are abundant under the canopy of P. laevigata in an intertropical semi-arid zone in Central Mexico. Our results on the gut content analysis of these oribatid species resulted in the ITS-based identification of the following fungi: Aspergillus homomorphus, Beauveria bassiana, Filobasidium sp., Mortierella sp., Roussoella sp., Saccharomyces cerevisiae, Sclerotiniaceae sp. and Triparticalcar sp. Furthermore, under laboratory conditions both oribatid mite species exhibited feeding preferences on melanized fungi, such as Cladosporium spp., whereas A. homomorphus and Fusarium penzigi were avoided. Our findings indicated that the analyzed oribatid mite species have similar feeding preferences for melanized fungi, which might suggest resource partitioning and a degree of preference, explaining the coexistence of both oribatid species.

No complementarity no gain-Net diversity effects on tree productivity occur once complementarity emerges during early stand development.

Although there is compelling evidence that tree diversity has an overall positive effect on forest productivity, there are important divergences among studies on the nature and strength of these diversity effects and their timing during forest stand development. To clarify conflicting results related to stand developmental stage, we explored how diversity effects on productivity change through time in a diversity experiment spanning 11 years. We show that the strength of diversity effects on productivity progressively increases through time, becoming significantly positive after 9 years. Moreover, we demonstrate that the strengthening of diversity effects is driven primarily by gradual increases in complementarity. We also show that mixing species with contrasting resource-acquisition strategies, and the dominance of deciduous, fast-developing species, promote positive diversity effects on productivity. Our results suggest that the canopy closure and subsequent stem exclusion phase are key for promoting niche complementarity in diverse tree communities.

Coupling of plant and mycorrhizal fungal diversity: its occurrence, relevance, and possible implications under global change.

First principles predict that diversity at one trophic level often begets diversity at other levels, suggesting plant and mycorrhizal fungal diversity should be coupled. Local-scale studies have shown positive coupling between the two, but the association is less consistent when extended to larger spatial and temporal scales. These inconsistencies are likely due to divergent relationships of different mycorrhizal fungal guilds to plant diversity, scale dependency, and a lack of coordinated sampling efforts. Given that mycorrhizal fungi play a central role in plant productivity and nutrient cycling, as well as ecosystem responses to global change, an improved understanding of the coupling between plant and mycorrhizal fungal diversity across scales will reduce uncertainties in predicting the ecosystem consequences of species gains and losses.

Relationship between the vertical distribution of fine roots and residual soil nitrogen along a gradient of hardwood mixture in a conifer plantation.

In forest ecosystems, understanding the relationship between the vertical distribution of fine roots and residual soil nitrogen is essential for clarifying the diversity-productivity-water purification relationship. Vertical distributions of fine-root biomass (FRB) and concentrations of nitrate-nitrogen (NO3 -N) in soil water were investigated in a conifer plantation with three thinning intensities (Control, Weak and Intensive), in which hardwood abundance and diversity were low, moderate and high, respectively. Intensive thinning led to the lowest NO3 -N concentration in soil water at all depths (0-100 cm) and highest FRB at shallow depths (0-50 cm). The NO3 -N concentration at a given depth was negatively correlated with total FRB from the surface to the depth at which NO3 -N concentration was measured, especially at shallow depths, indicating that more abundant fine roots led to lower levels of downward NO3 -N leaching. FRB contributed positively to nitrogen content of hardwood leaves. These findings demonstrate that a hardwood mixture in conifer plantations resulted in sufficient uptake of NO3 -N from soil by well developed fine-root systems, and translocation to canopy foliage. This study suggests that productivity and water purification can be achieved through a hardwood mixture in conifer plantations.

Inter-cluster competition and resource partitioning may govern the ecology of Frankia.

Microbes live in a complex communal ecosystem. The structural complexity of microbial community reflects diversity, functionality, as well as habitat type. Delineation of ecologically important microbial populations along with exploration of their roles in environmental adaptation or host-microbe interaction has a crucial role in modern microbiology. In this scenario, reverse ecology (the use of genomics to study ecology) plays a pivotal role. Since the co-existence of two different genera in one small niche should maintain a strict direct interaction, it will be interesting to utilize the concept of reverse ecology in this scenario. Here, we exploited an 'R' package, the RevEcoR, to resolve the issue of co-existing microbes which are proven to be a crucial tool for identifying the nature of their relationship (competition or complementation) persisting among them. Our target organism here is Frankia, a nitrogen-fixing actinobacterium popular for its genetic and host-specific nature. According to their plant host, Frankia has already been sub-divided into four clusters C-I, C-II, C-III, and C-IV. Our results revealed a strong competing nature of CI Frankia. Among the clusters of Frankia studied, the competition index between C-I and C-III was the largest. The other interesting result was the co-occurrence of C-II and C-IV groups. It was revealed that these two groups follow the theory of resource partitioning in their lifestyle. Metabolic analysis along with their differential transporter machinery validated our hypothesis of resource partitioning among C-II and C-IV groups.

Reef fishes weaken dietary preferences after coral mortality, altering resource overlap.

The direct and indirect effects of climate change can affect, and are mediated by, changes in animal behaviour. However, we often lack sufficient empirical data to assess how large-scale disturbances affect the behaviour of individuals, which scales up to influence communities. Here, we investigate these patterns by focusing on the foraging behaviour of butterflyfishes, prominent coral-feeding fishes on coral reefs, before and after a mass coral bleaching event in Iriomote, Japan. In response to 65% coral mortality, coral-feeding fishes broadened their diets, showing a significant weakening of dietary preferences across species. Multiple species reduced their consumption of bleaching-sensitive Acropora corals, while expanding their diets to consume a variety of other coral genera. This resulted in decreased dietary overlap among butterflyfishes. Behavioural changes in response to bleaching may increase resilience of coral reef fishes in the short term. However, coral mortality has reduced populations of coral-feeders world-wide, indicating the changes in feeding behaviour we document here may not be sufficient to ensure long-term resilience of butterflyfishes on coral reefs.

Sex-specific flower resource use in hummingbird communities.

Research Highlight: Maglianesi, M. A., Maruyama, P. K., Temeles, E. J., Schleuning, M., Zanata, T. B., Sazima, M., Gutiérrez-Zamora, A., Marín-Gómez, O. H., Rosero-Lasprilla, L., Ramírez-Burbano, M. B., Ruffini, A. E., Salamanca-Reyes, J. R., Sazima, I., Nuñez-Rosas, L. E., Arizmendi, M. C., Rahbek, C., & Dalsgaard, B. (2022). Behavioural and morphological traits influence sex-specific floral resource use by hummingbirds across the Americas. Journal of Animal Ecology, 00: 00-00. https://doi.org/10.1111/1365-2656.13746. In their paper on intersexual differences in niche breadth and niche overlap in floral resource use in hummingbird communities, Maglianesi et al. compiled data of plant-hummingbird interactions based on pollen loads, and territoriality and morphological traits for 31 hummingbird species, and investigated whether patterns of resource use by females and males were related to sexual dimorphism and foraging behaviour. While accounting for evolutionary relatedness among species, the authors found a high level of resource partitioning between sexes (broader and more dissimilar floral niche breadth in females) and the sex-specific resource use by hummingbird species was related to territoriality and morphological traits; niche overlap between sexes was greater for territorial than non-territorial species, and lower for species with greater sexual dimorphism in bill curvature. This paper addresses two very timely issues, on the one hand on resource partitioning by sex to reduce intersexual competition in hummingbirds, and on the other hand highlight the much needed information on foraging ecology of female hummingbirds for better understanding of intersexual variation in shaping coexistence and species diversity in hummingbird communities and the interactions between plants and their hummingbird pollinators.

Investigación Destacada: Maglianesi, M. A., Maruyama, P. K., Temeles, E. J., Schleuning, M., Zanata, T. B., Sazima, M., Gutiérrez-Zamora, A., Marín-Gómez, O. H., Rosero-Lasprilla, L., Ramírez-Burbano, M. B., Ruffini, A. E., Salamanca-Reyes, J. R., Sazima, I., Nuñez-Rosas, L. E., Arizmendi, M. C., Rahbek, C., & Dalsgaard, B. (2022). Behavioural and morphological traits influence sex-specific floral resource use by hummingbirds across the Americas. Journal of Animal Ecology, 00: 00-00. https://doi.org/10.1111/1365-2656.13746. En su artículo sobre las diferencias intersexuales en la amplitud de nicho y la superposición de nicho en el uso de recursos florales en las comunidades de colibríes, Maglianesi et al. recopilaron datos de interacciones planta-colibrí basados en cargas de polen, y de territorialidad y rasgos morfológicos para 31 especies de colibríes, e investigaron si los patrones de uso de recursos por parte de hembras y machos estaban relacionados con el dimorfismo sexual y la conducta de forrajeo. Al tener en cuenta la relación evolutiva entre las especies, los autores encontraron un alto nivel de partición de recursos entre sexos (amplitud de nicho floral más amplio y diferente en las hembras) y el uso de recursos específico del sexo por parte de las especies de colibríes estaba relacionado con la territorialidad y los rasgos morfológicos; la superposición de nicho entre sexos fue mayor para las especies territoriales que para las no territoriales, y menor para las especies con mayor dimorfismo sexual en la curvatura del pico. Este artículo aborda dos temas muy oportunos, por un lado, la partición de recursos por sexo para reducir la competencia intersexual en los colibríes y, por otro lado, destaca la necesidad de información sobre la ecología de alimentación de las hembras de los colibríes para una mejor comprensión de la variación intersexual en la conformación de la coexistencia y diversidad de especies en las comunidades de colibríes y las interacciones entre las plantas y sus colibríes polinizadores.

Sum of fears among intraguild predators drives the survival of green sea turtle (Chelonia mydas) eggs.

Ecologists have long theorized that apex predators stabilize trophic systems by exerting a net protective effect on the basal resource of a food web. Although experimental and observational studies have borne this out, it is not always clear what behavioural mechanisms among the trophically connected species are responsible for this stability. Fear of intraguild predation is commonly identified as one such mechanism in models and mesocosm studies, but empirical evidence in natural systems remains limited, as the complexity of many trophic systems renders detailed behavioural studies of species interactions challenging. Here, we combine long-term field observations of a trophic system in nature with experimental behavioural studies of how all the species in this system interact, in both pairs and groups. The results demonstrate how an abundant, sessile and palatable prey item (sea turtle eggs, Chelonia mydas) survives when faced by three potential predators that all readily eat eggs: an apex predator (the stink ratsnake, Elaphe carinata) and two mesopredators (the brown rat, Rattus norvegicus, and kukri snake, Oligodon formosanus). Our results detail how fear of intraguild predation, conspecific cannibalism, habitat structure and territorial behaviour among these species interact in a complex fashion that results in high egg survival.

Are stable isotope ratios suitable for describing niche partitioning and individual specialization?

As concerns about anthropogenic and natural disturbance grow, understanding animal resource use patterns has been increasingly prioritized to predict how changes in environmental conditions, food web structure, and population dynamics will affect biological resilience. Among the tools used to assess resource use, stable isotope analysis has proliferated in ecological studies, particularly in relation to describing intra- and interspecific variation in trophic interactions. Despite a growing need to disseminate scientific information, the inherent limitations of stable isotope ratios and inappropriate synonymizing of distinct evolutionary and ecological processes may mislead ecological inferences in natural systems. This situation necessitates a re-evaluation of the utility of stable isotope ratios to address certain ecological questions. Here, we assess the efficacy of stable isotope ratios to describe two fundamental ecological processes, niche partitioning and individual specialization. Investigation of these processes has increased substantially in accordance with increased access to stable isotope data. This article discusses the circumstances and approaches that are necessary to evaluate niche partitioning and individual specialization, and outlines key considerations for the associated application of stable isotope ratios.

Rich and abundant spider communities result from enhanced web capture breadth and reduced overlap in urban greenspaces.

Urbanization is a key contributor to biodiversity loss, but evidence is mounting that cities can support rich arthropod communities, including rare and threatened species. Furthermore, greenspace is growing within hundreds of "shrinking cities" that have lost population resulting in a need to demolish an overabundance of infrastructure creating vacant land. Efforts are underway to transform vacant lots, often viewed as blighted areas, into habitats that promote biodiversity and generate ecosystem services, such as urban agroecosystems. To understand how reconfiguring these greenspaces might influence species conservation, elucidation of the factors that drive the distribution of an urban species pool is needed. In particular, the importance of species interactions in structuring urban communities is poorly understood. We tested hypotheses that (1) greater breadth of prey captured by web-building spiders and reduced overlap of prey capture among individuals facilitates the conservation of genera richness and abundance and (2) heterogeneity within a greenspace patch facilitates enhanced dietary niche breadth and greater resource partitioning. In 2013 and 2014, the abundance, breadth and degree of overlap in prey capture of sheet web spiders (Linyphiidae) was measured using web mimic traps at 160 microsites (0.25 m2 ) situated in four urban vacant lots and four urban farms in the city of Cleveland, Ohio, USA. Within a subset of 40 microsites, we used vacuum sampling and hand collection to measure the abundance and genera richness of Linyphiidae. Spider richness and abundance were significantly reduced within urban farms relative to vacant lots. The distribution of spiders and prey was explained by habitat structure, with microsites dominated by tall grasses and flowering plants, with a high bloom abundance and richness, supporting greater prey capture and a higher genera richness and abundance of spiders. In 2014, web capture overlap was significantly greater within microsites dominated by bare ground. These findings illustrate that urban greenspace conservation efforts that focus on reducing bare ground and incorporating a diversity of grasses and flowering plant species can promote linyphiid spiders, potentially by relaxing exploitative competition for shared prey.

Does ecological release from distantly related species affect phenotypic divergence in brook charr?

Ecological opportunity occurs when a resource becomes available through a decrease of interspecific competition and another species colonizes the vacant niche through phenotypic plasticity and intraspecific competition. Brook charr exhibit a resource polymorphism in some Canadian Shield lakes, where a littoral ecotype feeds mainly on zoobenthos and a pelagic ecotype feeds mostly on zooplankton. The objectives of this study were to test that (i) resource polymorphism is common in these brook charr populations, (ii) the presence creek chub and white sucker, two introduced species competing with brook charr for littoral resources, will decrease the phenotypic divergence between the two brook charr ecotypes, and (iii) the ecological release from introduced species will increase population and/or individual niche widths in brook charr. The study was based on 27 lakes and five indicators of resource use (stomach content, liver δ13C, muscle astaxanthin concentration, pyloric caecum length, and gill raker length). Our results indicate that within-lake differences in resource use by both ecotypes are common and stable through time. When facing interspecific competition, both littoral and pelagic brook charr incorporated more pelagic prey into their diet but maintained the amplitude of their differences in resource use, which contradicts our second prediction. Finally, we did not find any significant effect of introduced species on population and individual niche widths of brook charr. We suggest that the difference in feeding mode among distantly related competitors could prevent the complete exclusion of a species from a given niche and explain the lack of response to the ecological release.