A prion-like protein regulator of seed germination undergoes hydration-dependent phase separation.

Many organisms evolved strategies to survive desiccation. Plant seeds protect dehydrated embryos from various stressors and can lay dormant for millennia. Hydration is the key trigger to initiate germination, but the mechanism by which seeds sense water remains unresolved. We identified an uncharacterized Arabidopsis thaliana prion-like protein we named FLOE1, which phase separates upon hydration and allows the embryo to sense water stress. We demonstrate that biophysical states of FLOE1 condensates modulate its biological function in vivo in suppressing seed germination under unfavorable environments. We find intragenic, intraspecific, and interspecific natural variation in FLOE1 expression and phase separation and show that intragenic variation is associated with adaptive germination strategies in natural populations. This combination of molecular, organismal, and ecological studies uncovers FLOE1 as a tunable environmental sensor with direct implications for the design of drought-resistant crops, in the face of climate change.

Robust Thiazole-Linked Covalent Organic Frameworks for Water Sensing with High Selectivity and Sensitivity.

The rational design of covalent organic frameworks (COFs) with hydrochromic properties is of significant value because of the facile and rapid detection of water in diverse fields. In this report, we present a thiazole-linked COF (TZ-COF-6) sensor with a large surface area, ultrahigh stability, and excellent crystallinity. The sensor was synthesized through a simple three-component reaction involving amine, aldehyde, and sulfur. The thiazole and methoxy groups confer strong basicity to TZ-COF-6 at the nitrogen sites, making them easily protonated reversibly by water. Therefore, TZ-COF-6 displayed color change visible to the naked eye from yellow to red when protonated, along with a red shift in absorption in the ultraviolet-visible diffuse reflectance spectra (UV-vis DRS) when exposed to water. Importantly, the water-sensing process was not affected by polar organic solvents, demonstrating greater selectivity and sensitivity compared to other COF sensors. Therefore, TZ-COF-6 was used to detect trace amounts of water in organic solvents. In strong polar solvents, such as N,N-dimethyl formamide (DMF) and ethanol (EtOH), the limit of detection (LOD) for water was as low as 0.06% and 0.53%, respectively. Even after 8 months of storage and 15 cycles, TZ-COF-6 retained its original crystallinity and detection efficiency, displaying high stability and excellent cycle performance.

Yellow Carbon Dots for Fluorescent Water Sensing, Relative Humidity Sensing, and Anticounterfeiting Applications.

Most fluorescent probes based on carbon dots (CDs) fluorescence color or intensity change are still used for detection in solution, but in practical fluorescence detection applications, detection in the solid state is necessary. Therefore, a CDs-based fluorescence sensing device is designed in this paper, which can be used for water detection in liquid and solid states. Using oPD as a single precursor, yellow fluorescent CDs (y-CDs) were prepared by hydrothermal method, which can be used in the field of water detection and anti-counterfeiting by using its solvent-sensitive properties. First, y-CDs can be used to visually and intelligently detect the water content in ethanol. Secondly, it can be used to detect the Relative Humidity (RH) of the environment by combining it with cellulose to form a fluorescent film. Finally, y-CDs can also be used as a fluorescent material for fluorescence anti-counterfeiting.

Fluorescence Sensor for Water in Organic Solvent Using Graphene Oxide- Rhodamine B and Cucurbit[7]uril.

Detection of water in organic solvents gained much importance as these solvents have been used as a medium for conducting organic reactions and water was considered as an inhibitor, when it is present in the reaction medium. There are number of methods available to measure the water content in organic solvents, however, such methods are time consuming and expensive. Here, we developed a facile method for detecting water in organic solvents using an inexpensive fluorescent probe - Rhodamine B decorated Graphene oxide (RBGO). The fluorescent probe, RBGO can be conveniently prepared by mixing the rhodamine B (RB) with graphene oxide (GO) in water. However, the probe will function as a sensor for water in the organic solvents through the release of dye upon interaction with the water present in organic solvents. Surprisingly, combination of cucurbit[7]uril (CB[7]) and RBGO increased the sensitivity of this sensor dramatically for the detection of water. This is the first example of water sensor with best detection limit by the involvement of host molecules such as CB[7]. This sensor displayed the low limit of detection (LOD) for organic solvents (LOD: 0.0015% for DMSO, 0.0025% for DMF), through the two-way process such as decomplexation and encapsulation. We presume that the role of CB [7] can be implemented in other similar sensors to enhance the sensitivity.

Reversible Luminescent Switching Induced by Heat/Water Treatment in a Zero-Dimensional Hybrid Antimony(â ¢) Chloride.

Recently zero-dimensional (0-D) inorganic-organic metal halides (IOMHs) have become a promising class of optoelectronic materials. Herein, we report a new photoluminescent (PL) 0-D antimony(III)-based IOMH single crystal, namely [H2BPZ][SbCl5]·H2O (BPZ = benzylpiperazine). Photophysical characterizations indicate that [H2BPZ][SbCl5]·H2O exhibits singlet/triplet dual-band emission. Density functional theory (DFT) calculations suggest that [H2BPZ][SbCl5]·H2O has the large energy difference between singlet and triplet states, which might induce the dual emission in this compound. Temperature-dependent PL spectra analyses suggest the soft lattice and strong electron-phonon coupling in this compound. Thermogravimetric analysis shows that the water molecules in the lattice of the title crystal could be removed by thermal treatment, giving rise to a dehydrated phase of [H2BPZ][SbCl5]. Interestingly, such structural transformation is accompanied by a reversible PL emission transition between red light (630 nm, dehydrated phase) and yellow light (595 nm, water-containing phase). When being exposed to an environment with 77% relative humidity, the emission color of the dehydrated phase was able to change from red to yellow within 20 s, and the red emission could be restored after reheating. The red to yellow emission switching could be achieved in acetone with water concentration as low as 0.2 vol%. The reversible PL transition phenomenon makes [H2BPZ][SbCl5]·H2O a potential material for luminescent water-sensing.

Determination of water in organic solvents and raw food products by fluorescence quenching of a crystalline vinyl-functionalized COF.

Covalent organic frameworks (COFs) with good chemical stability, flexible chemical functionalization, tunable pore sizes, and high specific surface areas have been increasingly employed in the field of fluorescence sensing. In this work, a crystalline vinyl-functionalized COF TzDa-V was facilely prepared through a room-temperature synthetic method via condensation reaction between 4,4',4″-(1,3,5-triazine-2,4,6-triyl)trianiline (Tz) and 2,5-diallyloxyterephthalaldehyde (Da-V). The intermolecular charge transfer (ICT) effect endowed the TzDa-V with fluorescence characteristic, and it was sensitive to trace water and can be quenched due to the disruption of ICT process by water. On this base, the prepared COF TzDa-V with excellent chemical/thermal stability was applied to sensing of trace water in common organic solvents such as DMF, acetone, THF, and ethyl acetate with rapid response (less than 10 s), satisfactory sensing range (0.5-18% water in DMF, 0.5-15% water in acetone, 0.5-16% water in THF, 0.5-5% in ethyl acetate, v/v), and high sensitivity. The limits of detection for water in DMF, acetone, THF, and ethyl acetate were 0.0497%, 0.0590%, 0.0502%, and 0.0766% (v/v), respectively. The proposed probe was successfully used for the detection of trace water in food products such as salt and sugar. The COF TzDa-V would be a good candidate for application in water sensing.

7-Dialkylaminocoumarin Oximates: Small Molecule Fluorescent "Turn-On" Chemosensors for Low-Level Water Content in Aprotic Organic Solvents.

The water sensing properties of two efficient two-component fluorescent "turn-on" chemo-sensors based on the 7-dialkylaminocoumarin oxime acid-base equilibrium were investigated. Interestingly, although simple frontier orbital analysis predicts an intramolecular photoinduced electron transfer quenching pathway in conjugated oximates, TD-DFT (Time-dependent density functional theory) quantum chemical calculations support non-radiative dark S1 excited state deactivation as a fluorescence quenching mechanism. Due to the acid-base sensing mechanism and sensitive "turn-on" fluorescent response, both studied coumarin aldoxime chemosensors exhibit rapid response to low-level water content in polar aprotic solvents, with detection limits comparable to chemodosimeters or chemosensors based on interpolymer π-stacking aggregation.

Deep blue emitting dual state fluorescent triphenylamine-dicyclohexylurea derivative: Multi-stimuli responsive fluorescence switching and methanol/water sensing.

A new deep blue emissive organic fluorophore (N-cyclohexyl-N-(cyclohexylcarbamoyl)-4-(diphenylamino)benzamide (NCDPB)) was designed and synthesized, which showed strong fluorescence both in solution and solid-state. Solid-state structural analysis of NCDPB revealed non-planar twisted molecular conformation with extended hydrogen bonding between the amide functionalities. The propeller shaped triphenylamine (TPA) and non-planar cyclohexyl unit prevented close π…π stacking and produced strong deep blue emission in the solid state (λmax = 400 nm, quantum yield (Φf) = 12.6 %). NCDPB also exhibited strong solvent polarity dependent tunable emission in solution (λmax = 402-462 nm, Φf = 1.15 (compared to quinine sulphate)). NCDPB showed reversible fluorescence switching between two fluorescence states upon mechanical crushing and heating/solvent exposure. Mechanical crushing caused red shifting of fluorescence from 400 to 447 nm and heating/solvent exposure reversed the fluorescence. Further, NCDPB also displayed off-on reversible/self-reversible fluorescence switching upon exposure to trifluoracetic acid (TFA) and NH3. The repeated fluorescence switching cycles indicated high reversibility without any significant change of fluorescence intensity. The drastically different fluorescence of NCDPB in CH3OH and EtOH was utilized to distinguish them and monitor CH3OH contamination in ethanol and benzene. It showed limit of detection (LOD) of methanol up to 0.25 % and 7 % in benzene and ethanol, respectively. The water sensitive fluorescence modulation of NCDPB in organic solvents was used to sensing water contamination in common organic solvents. Thus, integration of twisted TPA with H-bonding urea produced dual state emitting organic fluorophore with multi-responsive fluorescence switching and solvent sensing.

Water sensitive fluorescence tuning of V-shaped ESIPT fluorophores: Substituent effect and trace amount water sensing in DMSO.

Highly sensitive nature of excited state intramolecular proton transfer (ESIPT) functionality in organic fluorophores made them potential candidates for developing environmental sensors and bioimaging applications. Herein, we report the synthesis of V-shaped Dapsone based Schiff base ESIPT derivatives (1-3) and water sensitive wide fluorescence tuning from blue to red in DMSO. Solid-state structural analysis confirmed the V-shaped molecular structure with intramolecular H-bonding and substituent dependent molecular packing in the crystal lattice. 1 showed strong solid-state fluorescence (λmax = 554 nm, Φf = 21.2 %) whereas methoxy substitution (2 and 3) produced tunable but significantly reduced fluorescence (λmax = 547 (2) and 615 nm (3), Φf = 2.1 (2) and 6.5 % (3)). Interestingly, aggregation induced emission (AIE) studies in DMSO-water mixture revealed water sensitive fluorescence tuning. The trace amount of water (less than 1 %) in DMSO converted the non-emissive 1-3 into highly emissive state due to keto tautomer formation. Further increasing water percentage produced deprotonated state of 1-3 in DMSO and enhanced the fluorescence intensity with red shifting of emission peak. At higher water fraction, 1-3 in DMSO produced aggregates and red shifted the emission with reduction of fluorescence intensity. The concentration dependent fluorescence study revealed the very low detection limit of water in DMSO. The limit of detection (LOD) of 1, 2 and 3 were 0.14, 1.04 and 0.65 % of water in DMSO. Hence, simple Schiff bases of 1-3 showed water concentration dependent keto isomer, deprotonated and aggregated state tunable fluorescence in DMSO. Further, scanning electron microscopic (SEM) studies of 1-3 showed water concentration controlled self-assembly and tunable fluorescence.

Terahertz spectroscopic monitoring and analysis of citrus leaf water status under low temperature stress.

Low temperature stress, in the form of chilling and freezing, is one of the major environmental factors impacting on citrus yield, which changes plant's water state and results in the crops' sub-health or injury. The innovative terahertz (THz) spectroscopy and imaging based sensing technology has been shown to be a suitable tool for plant leaf water status determination, due to THz radiation's innate sensitivity to hydrogen bond vibration in aqueous solutions, which is usually related to plant phenotype change. We demonstrate experimentally that the THz absorption coefficient of leaf could be used for distinguishing plant's physiological stress status, exhibiting clear decreasing or increasing trend under chilling or freezing stress respectively. The underlying rationale might be that membrane damage shows a diverse pattern, changing the intra- or extra-cellular liquid environments, likely being linked to the various THz spectral characteristics. There were different adaptations in leaf morphology, leading to different leaf density, which in turn affects the water volume fraction. Moreover, different patterns of the dynamic equilibrium state of free water and bound water under chilling and freezing treatment were revealed by THz spectroscopy. Here, THz spectroscopic monitoring has shown unique potential for judging citrus's low temperature stress state through bio-water detection and discrimination.

Sensor feedback system enables automated deficit irrigation scheduling for cotton.

Precision irrigation technologies using sensor feedback can provide dynamic decision support to help farmers implement DI strategies. However, few studies have reported on the use of these systems for DI management. This two-year study was conducted in Bushland, Texas to investigate the performance of the geographic information (GIS) based irrigation scheduling supervisory control and data acquisition (ISSCADA) system as a tool to manage deficit irrigation scheduling for cotton (Gossypim hirsutum L). Two different irrigation scheduling methods automated by the ISSCADA system - (1) a plant feedback (designated C) - based on integrated crop water stress index (iCWSI) thresholds, and (2) a hybrid (designated H) method, created to combine soil water depletion and the iCWSI thresholds, were compared with a benchmark manual irrigation scheduling (M) that used weekly neutron probe readings. Each method applied irrigation at levels designed to be equivalent to 25%, 50% and 75% replenishment of soil water depletion to near field capacity (designated I25, I50 and I75) using the pre-established thresholds stored in the ISSCADA system or the designated percent replenishment of soil water depletion to field capacity in the M method. Fully irrigated and extremely deficit irrigated plots were also established. Relative to the fully irrigated plots, deficit irrigated plots at the I75 level for all irrigation scheduling methods-maintained seed cotton yield, while saving water. In 2021, the irrigation savings was a minimum of 20%, while in 2022, the minimum savings was 16%. Comparing the performance of deficit irrigation scheduling between the ISSCADA system and the manual method showed that crop response for all three methods were statistically similar at each irrigation level. Because the M method requires labor intensive and expensive use of the highly regulated neutron probe, the automated decision support provided by the ISSCADA system could simplify deficit irrigation management of cotton in a semi-arid region.

Reversible fluorescent test strip with red fluorescent carbon dots for monitoring water in organic solvents: Visual detection via a smartphone.

Herein, a novel type of red-emitting carbon dots called TN-CDs was created via a one-step hydrothermal approach using neutral red and tartaric acid as raw materials. The fluorescence of TN-CDs was gradually quenched as the amount of water increased, and the color of the solution changed from yellow to pink mauve (or purple to pink). The reaction could be completed within only 5 s in various organic solvents such as N,N-Dimethylformamide (DMF), methanol (MeOH), acetonitrile (ACN), and ethanol (EtOH) with linear detection ranges of 1.2%-35.0%, 0.5%-20.0%, 0.25%-5.0% and 0%-16.0%, respectively. In addition, we prepared a reusable test strip and then combined it with TN-CDs to detect water content in DMF, as well as integrated it with smartphone software, a UV lamp, and a dark chamber for real-time, on-site, visual quantitative detection of the water content.

Highly Crystalline Covalent Organic Frameworks Act as a Dual-Functional Fluorescent-Sensing Platform for Myricetin and Water, and Adsorbents for Myricetin.

Selective detection of active ingredients in complex samples has always been a crucial challenge because there are many disturbing compounds, especially structural analogues that interfere with the detection. In this work, a fluorescent covalent organic framework (named COF-TD), which can be used for the selective fluorescence detection and enrichment of myricetin from complex samples, was reported for the first time. The highly crystalline COF-TD with bright blue fluorescence was formed through a solution polymerization method by the condensation reaction between 4,4',4″-(1,3,5-triazine-2,4,6-triyl)trianiline and 2,5-dihydroxy-1,4-benzenedicarboxaldehyde. Due to spatial size selectivity, multisites hydrogen bonding, and π-π interaction, myricetin can quench the fluorescence of COF-TD with an inner filter effect (IFE) and static quenching mechanisms as well as can be enriched on COF-TD. Myricetin can observably eliminate the interference of other compounds and selectively quench the fluorescence of COF-TD with a limit of detection (LOD) of 0.30 µg·mL-1. The high adsorption ability of COF-TD (Q = 124.6 mg·g-1) to myricetin was also obtained. Finally, a sensing platform based on COF-TD for myricetin was successfully developed and applied for the detection of myricetin from vine teas. In addition, COF-TD also showed good water sensing ability and could be used effectively to detect water content in organic solvent (1-18% water in acetone, 0.5-5% water in acetonitrile, 1-4.5% water in ethyl acetate, v/v). To the best of our knowledge, this is the first report where COF-TD was used to detect water in a relatively wide concentration range. In all, this work provided dual-functional fluorescent COFs with the properties of an adsorbent, opening up new methodologies for the simple, selective, and enrichment detection method for myricetin.

A Colorimetric Chemosensor Based on a Nozoe Azulene That Detects Fluoride in Aqueous/Alcoholic Media.

Colorimetry is an advantageous method for detecting fluoride in drinking water in a resource-limited context, e. g., in parts of the developing world where excess fluoride intake leads to harmful health effects. Here we report a selective colorimetric chemosensor for fluoride that employs an azulene as the reporter motif and a pinacolborane as the receptor motif. The chemosensor, NAz-6-Bpin, is prepared using the Nozoe azulene synthesis, which allows for its rapid and low-cost synthesis. The chemosensor gives a visually observable response to fluoride both in pure organic solvent and also in water/alcohol binary solvent mixtures.

Towards the Nanosheet-Based Photonic Nose: Vapor Recognition and Trace Water Sensing with Antimony Phosphate Thin Film Devices.

A 2D nanosheet-based photonic nose for vapor identification is presented. A HSbP2 O8 nanosheet thin-film sensor with resistive readout is developed for the tracking of trace amounts of water, and a photonic HSbP2 O8 /TiO2 multilayer structure is effective at optically distinguishing between chemically similar solvent vapors through analyte-specific host-guest interactions.