RESUMO
In a large number of plant species hydroxynitrile lyases catalyze the decomposition of cyanohydrins in order to generate hydrogen cyanide upon tissue damage. Hydrogen cyanide serves as a deterrent against herbivores and fungi. In vitro hydroxynitrile lyases are proficient biocatalysts for the stereospecific synthesis of cyanohydrins. Curiously, hydroxynitrile lyases from different species are completely unrelated in structure and substrate specificity despite catalyzing the same reaction. The hydroxynitrile lyase from almond shows close resemblance to flavoproteins of the glucose-methanol-choline oxidoreductase family. We report here 3D structural data of this lyase with the reaction product benzaldehyde bound within the active site, which allow unambiguous assignment of the location of substrate binding. Based on the binding geometry, a reaction mechanism is proposed that involves one of the two conserved active site histidine residues acting as a general base abstracting the proton from the cyanohydrin hydroxyl group. Site-directed mutagenesis shows that both active site histidines are required for the reaction to occur. There is no evidence that the flavin cofactor directly participates in the reaction. Comparison with other hydroxynitrile lyases reveals a large diversity of active site architectures, which, however, share the common features of a general active site base and a nearby patch with positive electrostatic potential. On the basis of the difference in substrate binding geometry between the FAD-dependent HNL from almond and the related oxidases, we can rationalize why the HNL does not act as an oxidase.
Assuntos
Aldeído Liases/química , Aldeído Liases/metabolismo , Flavina-Adenina Dinucleotídeo/química , Nitrilas/química , Nitrilas/metabolismo , Prunus/enzimologia , Acetonitrilas/química , Acetonitrilas/metabolismo , Aldeído Liases/genética , Sítios de Ligação , Catálise , Cristalização , Cristalografia por Raios X , Flavina-Adenina Dinucleotídeo/fisiologia , Hidrogenação , Ligantes , Modelos Moleculares , Mutagênese Sítio-Dirigida , Prunus/genética , Especificidade por SubstratoRESUMO
Traditional antitumor drugs inhibit the proliferation and metastasis of tumour cells by restraining the replication and expression of DNA. These drugs are usually highly cytotoxic. They kill tumour cells while also cause damage to normal cells at the same time, especially the hematopoietic cells that divide vigorously. Patients are exposed to other serious situations such as a severe infection caused by a decrease in the number of white blood cells. Energy metabolism is an essential process for the survival of all cells, but differs greatly between normal cells and tumour cells in metabolic pathways and metabolic intermediates. Whether this difference could be used as new therapeutic target while reducing damage to normal tissues is the topic of this paper. In this paper, we introduce five major metabolic intermediates in detail, including acetyl-CoA, SAM, FAD, NAD+ and THF. Their contents and functions in tumour cells and normal cells are significantly different. And the possible regulatory mechanisms that lead to these differences are proposed carefully. It is hoped that the key enzymes in these regulatory pathways could be used as new targets for tumour therapy.
Assuntos
Antineoplásicos/efeitos adversos , Carcinogênese/metabolismo , Neoplasias/metabolismo , Acetilcoenzima A/química , Acetilcoenzima A/metabolismo , Acetilcoenzima A/fisiologia , Proliferação de Células/efeitos dos fármacos , Progressão da Doença , Flavina-Adenina Dinucleotídeo/química , Flavina-Adenina Dinucleotídeo/metabolismo , Flavina-Adenina Dinucleotídeo/fisiologia , Humanos , NAD/química , NAD/metabolismo , NAD/fisiologia , Invasividade Neoplásica , Neoplasias/patologia , Neoplasias/terapia , S-Adenosilmetionina/química , S-Adenosilmetionina/metabolismo , Tetra-Hidrofolatos/química , Tetra-Hidrofolatos/metabolismo , Tetra-Hidrofolatos/fisiologiaRESUMO
The crystal structure of cholesterol oxidase, a 56kDa flavoenzyme was anisotropically refined to 0.95A resolution. The final crystallographic R-factor and R(free) value is 11.0% and 13.2%, respectively. The quality of the electron density maps has enabled modeling of alternate conformations for 83 residues in the enzyme, many of which are located in the active site. The additional observed structural features were not apparent in the previous high-resolution structure (1.5A resolution) and have enabled the identification of a narrow tunnel leading directly to the isoalloxazine portion of the FAD prosthetic group. The hydrophobic nature of this narrow tunnel suggests it is the pathway for molecular oxygen to access the isoalloxazine group for the oxidative half reaction. Resolving the alternate conformations in the active site residues provides a model for the dynamics of substrate binding and a potential oxidation triggered gating mechanism involving access to the hydrophobic tunnel. This structure reveals that the NE2 atom of the active site histidine residue, H447, critical to the redox activity of this flavin oxidase, acts as a hydrogen bond donor rather than as hydrogen acceptor. The atomic resolution structure of cholesterol oxidase has revealed the presence of hydrogen atoms, dynamic aspects of the protein and how side-chain conformations are correlated with novel structural features such as the oxygen tunnel. This new structural information has provided us with the opportunity to re-analyze the roles played by specific residues in the mechanism of the enzyme.
Assuntos
Colesterol Oxidase/química , Flavina-Adenina Dinucleotídeo/fisiologia , Oxigênio/química , Sítios de Ligação , Catálise , Colesterol Oxidase/metabolismo , Cristalização , Cristalografia por Raios X , Elétrons , Canais Iônicos/química , Modelos Moleculares , Conformação Molecular , Oxirredução , Ligação Proteica , Conformação Proteica , Streptomyces/enzimologiaRESUMO
In Klebsiella pneumoniae NifL antagonizes the action of the transcriptional activator NifA in the presence of molecular oxygen or combined nitrogen. To determine what cofactors might be involved in the oxygen sensing mechanism, we purified and analyzed fusion proteins made between the Escherichia coli maltose binding protein, MalE, and NifL. NifL synthesized and purified under strictly anaerobic conditions did not contain significant amounts of iron or acid-labile sulfur indicating the absence of an oxygen sensing iron-sulfur cluster. However, NifL protein purified in its inhibitory form contained 0.3 +/- 0.01 mol FAD and less than 0.01 mol FMN per mol NifL suggesting the presence of FAD as a cofactor. Characterization of NifL synthesized in the absence of oxygen and combined nitrogen showed that the non-inhibitory form of NifL also contained FAD (0.54 mol FAD per mol NifL). Using fusions between MalE and different portions of NifL we localized the binding site of FAD to the N-terminal domain of NifL. These results and our previous observation that the C-terminal domain of NifL is sufficient to inhibit NifA activity indicate that the N-terminally bound FAD is not directly required for the inhibitory activity of NifL. This observation is supported by the finding that purified apoprotein of NifL was still able to inhibit transcriptional activation by NifA in vitro.
Assuntos
Proteínas de Bactérias/química , Flavina-Adenina Dinucleotídeo/análise , Klebsiella pneumoniae/metabolismo , Nitrogênio/metabolismo , Oxigênio/metabolismo , Proteínas de Bactérias/antagonistas & inibidores , Proteínas de Bactérias/fisiologia , Flavina-Adenina Dinucleotídeo/fisiologia , Nitrogênio/farmacologia , Oxigênio/farmacologia , Proteínas Recombinantes de Fusão/fisiologia , Fatores de Transcrição/antagonistas & inibidoresRESUMO
The properties of two flavinogenesis enzymes--synthase of the aliphatic precursor of riboflavin (APR-synthase) and 6.7-dimethyl-8-ribityllumazinesynthase (DMRL-synthase) of Pichia guilliermondii. It is established that DMRL-synthase, uses APR as a substrate which contains, evidently, a phosphate group. The value of Km for APR is equal to 0.7.10(-5) M, for 2.4-dihydroxy-5-amino-6-ribitylaminopyrimidine--1.25.10(-5) M. It is riboflavin but not FAD that inhibits the activity of DMRL-synthase; the value (I)0.5 is equal to 2.10(-5) M. DMRL, riboflavin, flavin mononucleotide and FAD do not affect the APR-synthase activity. In iron-deficient cells of P. guilliermondii, Torulopsis candida, Debaryomyces klöckeri and Schwanniomyces occidentalis realizing the oversynthesis of riboflavin there occurs derepression of DMRL-synthase and APR-synthase.
Assuntos
Complexos Multienzimáticos/biossíntese , Pichia/enzimologia , Pteridinas/metabolismo , Riboflavina/biossíntese , Saccharomycetales/enzimologia , Candida/metabolismo , Candida/fisiologia , Flavina-Adenina Dinucleotídeo/fisiologia , Cinética , Complexos Multienzimáticos/antagonistas & inibidores , Pichia/metabolismo , Riboflavina/fisiologia , Leveduras/metabolismo , Leveduras/fisiologiaAssuntos
Reparo do DNA/genética , Desoxirribodipirimidina Fotoliase/fisiologia , Proteínas de Drosophila , Proteínas do Olho , Flavoproteínas/fisiologia , Família Multigênica , Células Fotorreceptoras de Invertebrados , Percepção Visual/genética , Animais , Proteínas de Bactérias/genética , Proteínas de Bactérias/fisiologia , Criptocromos , DNA/efeitos da radiação , Dano ao DNA , Desoxirribodipirimidina Fotoliase/genética , Drosophila melanogaster/enzimologia , Drosophila melanogaster/genética , Drosophila melanogaster/fisiologia , Escherichia coli/enzimologia , Escherichia coli/genética , Evolução Molecular , Flavina-Adenina Dinucleotídeo/fisiologia , Flavoproteínas/genética , Proteínas de Insetos/genética , Proteínas de Insetos/fisiologia , Mamíferos/genética , Mamíferos/metabolismo , Modelos Moleculares , Oxirredução , Filogenia , Proteínas de Plantas/genética , Proteínas de Plantas/fisiologia , Dímeros de Pirimidina/metabolismo , Receptores Acoplados a Proteínas G , Raios UltravioletaRESUMO
gamma-Secretase is known to play a pivotal role in the pathogenesis of Alzheimer disease through production of amyloidogenic Abeta42 peptides. Early onset familial Alzheimer disease mutations in presenilin (PS), the catalytic core of gamma-secretase, invariably increase the Abeta42:Abeta40 ratio. However, the mechanism by which these mutations affect gamma-secretase complex formation and cleavage specificity is poorly understood. We show that our in vitro assay system recapitulates the effect of PS1 mutations on the Abeta42:Abeta40 ratio observed in cell and animal models. We have developed a series of small molecule affinity probes that allow us to characterize active gamma-secretase complexes. Furthermore we reveal that the equilibrium of PS1- and PS2-containing active complexes is dynamic and altered by overexpression of Pen2 or PS1 mutants and that formation of PS2 complexes is positively correlated with increased Abeta42:Abeta40 ratios. These data suggest that perturbations to gamma-secretase complex equilibrium can have a profound effect on enzyme activity and that increased PS2 complexes along with mutated PS1 complexes contribute to an increased Abeta42:Abeta40 ratio.
Assuntos
Secretases da Proteína Precursora do Amiloide/fisiologia , Proteínas de Membrana/fisiologia , Presenilina-1/fisiologia , Presenilina-2/fisiologia , Doença de Alzheimer/enzimologia , Doença de Alzheimer/metabolismo , Doença de Alzheimer/fisiopatologia , Secretases da Proteína Precursora do Amiloide/metabolismo , Animais , Biotina/metabolismo , Linhagem Celular , Flavina-Adenina Dinucleotídeo/genética , Flavina-Adenina Dinucleotídeo/fisiologia , Técnicas de Introdução de Genes , Células HeLa , Humanos , Camundongos , Presenilina-1/metabolismo , Presenilina-2/metabolismoRESUMO
By using biochemical and structural analyses, we have investigated the catalytic mechanism of the recently discovered flavin-dependent thymidylate synthase ThyX from Paramecium bursaria chlorella virus-1 (PBCV-1). Site-directed mutagenesis experiments have identified several residues implicated in either NADPH oxidation or deprotonation activity of PBCV-1 ThyX. Chemical modification by diethyl pyrocarbonate and mass spectroscopic analyses identified a histidine residue (His53) crucial for NADPH oxidation and located in the vicinity of the redox active N-5 atom of the FAD ring system. Moreover, we observed that the conformation of active site key residues of PBCV-1 ThyX differs from earlier reported ThyX structures, suggesting structural changes during catalysis. Steady-state kinetic analyses support a reaction mechanism where ThyX catalysis proceeds via formation of distinct ternary complexes without formation of a methyl enzyme intermediate.
Assuntos
Chlorella/virologia , Flavina-Adenina Dinucleotídeo/fisiologia , Timidilato Sintase/química , Timidilato Sintase/fisiologia , Sequência de Aminoácidos , Substituição de Aminoácidos/genética , Arginina/metabolismo , Catálise , Sequência Conservada , Cristalografia por Raios X , Dietil Pirocarbonato/farmacologia , Inibidores Enzimáticos/farmacologia , Flavina-Adenina Dinucleotídeo/metabolismo , Ácido Glutâmico/metabolismo , Histidina/metabolismo , Cinética , Dados de Sequência Molecular , Phycodnaviridae/enzimologia , Phycodnaviridae/genética , Alinhamento de Sequência , Relação Estrutura-Atividade , Especificidade por Substrato , Timidilato Sintase/antagonistas & inibidores , Timidilato Sintase/genéticaRESUMO
By using styrene as the sole source of carbon and energy in concentrations of 10 to 500 microM, 14 strains of aerobic bacteria and two strains of fungi were isolated from various soil and water samples. In cell extracts of 11 of the bacterial isolates, a novel flavin adenine dinucleotide-requiring styrene monooxygenase activity that oxidized styrene to styrene oxide (phenyl oxirane) was detected. In one bacterial strain (S5), styrene metabolism was studied in more detail. In addition to styrene monooxygenase, cell extracts from strain S5 contained styrene oxide isomerase and phenylacetaldehyde dehydrogenase activities. A pathway for styrene degradation via styrene oxide and phenylacetaldehyde to phenylacetic acid is proposed.
Assuntos
Bactérias/metabolismo , Flavina-Adenina Dinucleotídeo/fisiologia , Oxigenases/fisiologia , Estirenos/metabolismo , Biodegradação Ambiental , Oxirredução , EstirenoRESUMO
The NIFL regulatory protein controls transcriptional activation of nitrogen fixation (nif) genes in Azotobacter vinelandii by direct interaction with the enhancer binding protein NIFA. Modulation of NIFA activity by NIFL, in vivo occurs in response to external oxygen concentration or the level of fixed nitrogen. Spectral features of purified NIFL and chromatographic analysis indicate that it is a flavoprotein with FAD as the prosthetic group, which undergoes reduction in the presence of sodium dithionite. Under anaerobic conditions, the oxidized form of NIFL inhibits transcriptional activation by NIFA in vitro, and this inhibition is reversed when NIFL is in the reduced form. Hence NIFL is a redox-sensitive regulatory protein and may represent a type of flavoprotein in which electron transfer is not coupled to an obvious catalytic activity. In addition to its ability to act as a redox sensor, the activity of NIFL is also responsive to adenosine nucleotides, particularly ADP. This response overrides the influence of redox status on NIFL and is also observed with refolded NIFL apoprotein, which lacks the flavin moiety. These observations suggest that both energy and redox status are important determinants of nif gene regulation in vivo.
Assuntos
Azotobacter vinelandii/genética , Proteínas de Bactérias/fisiologia , Regulação Bacteriana da Expressão Gênica , Genes Bacterianos , Fixação de Nitrogênio/genética , Difosfato de Adenosina/metabolismo , Flavina-Adenina Dinucleotídeo/fisiologia , Flavoproteínas/fisiologia , Conformação Proteica , Relação Estrutura-Atividade , Transcrição GênicaRESUMO
Flavine adenine dinucleotide (FAD) may inhibit not only the aggregation but also ATP release of platelets in vitro. FAD did not inhibit the synthesis of thromboxane from 1-14C-arachidonic acid by platelets. FAD was found to be hydrolyzed to FMN and AMP by a plasma enzyme, the activity of which in normal adults being estimated as 32.6 +/- 9.1 m microM/ml plasma/min and AMP, thus produced, and/or FAD might compete for the receptor sites on the platelet surface. This could be the mechanism of the inhibitory effect of FAD upon the platelet functions. The intravenous drip infusion of FAD in a dose of 0.2 mg/kg within 30 min was followed by no marked decrease of platelet aggregation, probably because the plasma concentration of FAD and/or AMP did not reach the levels high enough to suppress the platelet functions.
Assuntos
Plaquetas/fisiologia , Flavina-Adenina Dinucleotídeo/fisiologia , Trifosfato de Adenosina/sangue , Adulto , Ácido Araquidônico , Ácidos Araquidônicos/sangue , Plaquetas/enzimologia , Flavina-Adenina Dinucleotídeo/metabolismo , Humanos , Hidrólise , Agregação PlaquetáriaRESUMO
Hydrophobic interaction chromatography of coenzyme F420-reducing hydrogenase purified from Methanobacterium formicicum depleted protein-bound FAD and eliminated the ability to reduce coenzyme F420. Preincubation of the FAD-depleted hydrogenase with FAD restored 85% of the coenzyme F420-reducing activity. FMN did not replace FAD. A Kd of 12 microM was estimated for FAD. Analysis of the reactivated hydrogenase following molecular sieve column chromatography showed that FAD was bound to protein. The results indicate that protein-bound FAD is reversibly removed from the coenzyme F420-reducing hydrogenase and that this flavin is required for the reduction of coenzyme F420.
Assuntos
Euryarchaeota/enzimologia , Flavina-Adenina Dinucleotídeo/fisiologia , Oxirredutases/metabolismo , Riboflavina/análogos & derivados , Cromatografia/métodos , Hidrogenase , Oxirredução , Ligação Proteica , Riboflavina/metabolismoRESUMO
The iron-sulfur flavoenzyme adenylylsulfate (adenosine 5'-phosphosulfate, APS) reductase catalyzes reversibly the 2-electron reduction of APS to sulfite and AMP, a key step in the biological sulfur cycle. APS reductase from one archaea and three different bacteria has been purified, and the molecular and catalytic properties have been characterized. The EPR parameters and redox potentials (-60 and -520 mV versus NHE) have been assigned to the two [4Fe-4S] clusters I and II observed in the three-dimensional structure of the enzyme from Archaeoglobus fulgidus (Fritz, G., Roth, A., Schiffer, A., Büchert, T., Bourenkov, G., Bartunik, H. D., Huber, H., Stetter, K. O., Kroneck, P. M. H., and Ermler, U. (2002) Proc. Natl. Acad. Sci. U. S. A. 99, 1836-1841). Sulfite binds to FAD to form a covalent FAD N(5)-sulfite adduct with characteristic UV/visible spectra, in accordance with the three-dimensional structure of crystalline enzyme soaked with APS. UV/visible monitored titrations reveal that the substrates AMP and APS dock closely to the FAD cofactor. These results clearly document that FAD is the site of the 2-electron reduction of APS to sulfite and AMP. Reaction of APS reductase enzyme with sulfite and AMP leads to partial reduction of the [4Fe-4S] centers and formation of the anionic FAD semiquinone. Thus, both [4Fe-4S] clusters function in electron transfer and guide two single electrons from the protein surface to the FAD catalytic site.
Assuntos
Adenosina Fosfossulfato/metabolismo , Archaea/enzimologia , Bactérias/enzimologia , Flavina-Adenina Dinucleotídeo/fisiologia , Flavinas/metabolismo , Proteínas Ferro-Enxofre/fisiologia , Oxirredutases atuantes sobre Doadores de Grupo Enxofre , Oxirredutases/química , Catálise , Espectroscopia de Ressonância de Spin Eletrônica , Modelos Químicos , Modelos Moleculares , Oxirredução , Oxirredutases/metabolismo , Espectrofotometria UltravioletaRESUMO
Formate hydrogenlyase activity in a cell extract of Methanobacterium formicicum was abolished by removal of coenzyme F420; addition of purified coenzyme F420 restored activity. Formate hydrogenlyase activity was reconstituted with three purified components from M. formicicum: coenzyme F420-reducing hydrogenase, coenzyme F420-reducing formate dehydrogenase, and coenzyme F420. The reconstituted system required added flavin adenine dinucleotide (FAD) for maximal activity. Without FAD, the formate dehydrogenase and hydrogenase rapidly lost coenzyme F420-dependent activity relative to methyl viologen-dependent activity. Immunoadsorption of formate dehydrogenase or coenzyme F420-reducing hydrogenase from the cell extract greatly reduced formate hydrogenlyase activity; addition of the purified enzymes restored activity. The formate hydrogenlyase activity was reversible, since both the cell extract and the reconstituted system produced formate from H2 plus CO2 and HCO3-.
Assuntos
Aldeído Oxirredutases/metabolismo , Euryarchaeota/enzimologia , Formiato Desidrogenases/metabolismo , Hidrogenase/metabolismo , Complexos Multienzimáticos/metabolismo , Riboflavina/análogos & derivados , Estabilidade Enzimática , Euryarchaeota/metabolismo , Flavina-Adenina Dinucleotídeo/fisiologia , Técnicas de Imunoadsorção , Oxirredutases/metabolismo , Riboflavina/fisiologiaRESUMO
In the ferredoxin-NADP(+) reductase (FNR)/ferredoxin (Fd) system, an aromatic amino acid residue on the surface of Anabaena Fd, Phe-65, has been shown to be essential for the electron transfer (ET) reaction. We have investigated further the role of hydrophobic interactions in complex stabilization and ET between these proteins by replacing three hydrophobic residues, Leu-76, Leu-78, and Val-136, situated on the FNR surface in the vicinity of its FAD cofactor. Whereas neither the ability of FNR to accept electrons from NADPH nor its structure appears to be affected by the introduced mutations, different behaviors with Fd are observed. Thus, the ET interaction with Fd is almost completely lost upon introduction of negatively charged side chains. In contrast, only subtle changes are observed upon conservative replacement. Introduction of Ser residues produces relatively sizable alterations of the FAD redox potential, which can explain the modified behavior of these mutants. The introduction of bulky aromatic side chains appears to produce rearrangements of the side chains at the FNR/Fd interaction surface. Thus, subtle changes in the hydrophobic patch influence the rates of ET to and from Fd by altering the binding constants and the FAD redox potentials, indicating that these residues are especially important in the binding and orientation of Fd for efficient ET. These results are consistent with the structure reported for the Anabaena FNR.Fd complex.
Assuntos
Anabaena/enzimologia , Ferredoxina-NADP Redutase/metabolismo , Ferredoxinas/metabolismo , Flavina-Adenina Dinucleotídeo/fisiologia , Família Multigênica , Sequência de Aminoácidos , Transporte de Elétrons , Ferredoxina-NADP Redutase/química , Flavina-Adenina Dinucleotídeo/química , Cinética , Modelos Moleculares , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Oxirredução , Conformação Proteica , Homologia de Sequência de AminoácidosRESUMO
The membrane-associated coenzyme F420-reducing hydrogenase of Methanobacterium formicicum was purified 87-fold to electrophoretic homogeneity. The enzyme contained alpha, beta, and gamma subunits (molecular weights of 43,000, 36,700, and 28,800, respectively) and formed aggregates (molecular weight, 1,020,000) of a coenzyme F420-active alpha 1 beta 1 gamma 1 trimer (molecular weight, 109,000). The hydrogenase contained 1 mol of flavin adenine dinucleotide (FAD), 1 mol of nickel, 12 to 14 mol of iron, and 11 mol of acid-labile sulfide per mol of the 109,000-molecular-weight species, but no selenium. The isoelectric point was 5.6. The amino acid sequence I-N3-P-N2-R-N1-EGH-N6-V (where N is any amino acid) was conserved in the N-termini of the alpha subunits of the F420-hydrogenases from M. formicicum and Methanobacterium thermoautotrophicum and of the largest subunits of nickel-containing hydrogenases from Desulfovibrio baculatus, Desulfovibrio gigas, and Rhodobacter capsulatus. The purified F420-hydrogenase required reductive reactivation before assay. FAD dissociated from the enzyme during reactivation unless potassium salts were present, yielding deflavoenzyme that was unable to reduce coenzyme F420. Maximal coenzyme F420-reducing activity was obtained at 55 degrees C and pH 7.0 to 7.5, and with 0.2 to 0.8 M KCl in the reaction mixture. The enzyme catalyzed H2 production at a rate threefold lower than that for H2 uptake and reduced coenzyme F420, methyl viologen, flavins, and 7,8-didemethyl-8-hydroxy-5-deazariboflavin. Specific antiserum inhibited the coenzyme F420-dependent but not the methyl viologen-dependent activity of the purified enzyme.
Assuntos
Proteínas de Bactérias/isolamento & purificação , Euryarchaeota/enzimologia , Proteínas de Membrana/isolamento & purificação , Oxirredutases/isolamento & purificação , Riboflavina/análogos & derivados , Sequência de Aminoácidos , Proteínas de Bactérias/antagonistas & inibidores , Eletroforese em Gel de Poliacrilamida , Ativação Enzimática , Flavina-Adenina Dinucleotídeo/fisiologia , Soros Imunes/farmacologia , Cinética , Proteínas de Membrana/antagonistas & inibidores , Metais/isolamento & purificação , Dados de Sequência Molecular , Peso Molecular , Oxirredutases/antagonistas & inibidores , Riboflavina/metabolismoRESUMO
When cells are exposed to external H(2)O(2), the H(2)O(2) rapidly diffuses inside and oxidizes ferrous iron, thereby forming hydroxyl radicals that damage DNA. Thus the process of oxidative DNA damage requires only H(2)O(2), free iron, and an as-yet unidentified electron donor that reduces ferric iron to the ferrous state. Previous work showed that H(2)O(2) kills Escherichia coli especially rapidly when respiration is inhibited either by cyanide or by genetic defects in respiratory enzymes. In this study we established that these respiratory blocks accelerate the rate of DNA damage. The respiratory blocks did not substantially affect the amounts of intracellular free iron or H(2)O(2), indicating that that they accelerated damage because they increased the availability of the electron donor. The goal of this work was to identify that donor. As expected, the respiratory inhibitors caused a large increase in the amount of intracellular NADH. However, NADH itself was a poor reductant of free iron in vitro. This suggests that in non-respiring cells electrons are transferred from NADH to another carrier that directly reduces the iron. Genetic manipulations of the amounts of intracellular glutathione, NADPH, alpha-ketoacids, ferredoxin, and thioredoxin indicated that none of these was the direct electron donor. However, cells were protected from cyanide-stimulated DNA damage if they lacked flavin reductase, an enzyme that transfers electrons from NADH to free FAD. The K(m) value of this enzyme for NADH is much higher than the usual intracellular NADH concentration, which explains why its flux increased when NADH levels rose during respiratory inhibition. Flavins that were reduced by purified flavin reductase rapidly transferred electrons to free iron and drove a DNA-damaging Fenton system in vitro. Thus the rate of oxidative DNA damage can be limited by the rate at which electron donors reduce free iron, and reduced flavins become the predominant donors in E. coli when respiration is blocked. It remains unclear whether flavins or other reductants drive Fenton chemistry in respiring cells.