RESUMO
OBJECTIVE: The objective of this study was to examine the Borrelia seroprevalence among blood donors in Southeast Brazil. BACKGROUND: There is evidence that Borrelia spirochetes are circulating in Brazil; however, there are no studies that characterise these bacteria and investigate their seroprevalence in the Brazilian population. Such a situation, combined with a recent outbreak of tick-borne Rocky Mountain spotted fever in the São Paulo state demonstrates the increasing role of ticks as arthropod vectors in Brazil. METHODS: For the purpose of the study, 452 blood donors from Ribeirão Preto city, São Paulo state were tested using anti-Borrelia immunoglobulin G (IgG) assay. The positive results were also confirmed by Western blot for anti-borrelia IgM/IgG. RESULTS: The anti-Borrelia IgG enzyme-linked immunosorbent assay (ELISA) showed nine positive and nine borderline reactive samples, giving a total seroprevalence of 2·0% of anti-Borrelia IgG among Brazilian blood donors. The confirmation of the seropositive and borderline samples by Borrelia Western blot was demonstrated by IgG-positive results in 16 samples (a seroprevalence of 3.5%). Anti-Borrelia IgM antibodies were also detected in one sample. CONCLUSIONS: Our results demonstrate that Borrelia-like spirochetes may be circulating among blood donors from the São Paulo State and that the ticks have an important epidemiological role as vectors of bacterial infections in this Brazilian region. These results not only alert us to possible actions that might be undertaken in order to completely characterise the aetiological agents of Lyme-like syndromes in Brazil but also the possible impact that these bacterial agents might have on haemotherapy practices.
Assuntos
Anticorpos Antibacterianos/sangue , Doadores de Sangue , Infecções por Borrelia , Borrelia , Seleção do Doador , Imunoglobulina G/sangue , Adulto , Infecções por Borrelia/sangue , Infecções por Borrelia/epidemiologia , Brasil/epidemiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos SoroepidemiológicosRESUMO
We investigated whether Borrelia miyamotoi disease can be detected in its early stage by using PCR for borrelial 16S rRNA, which molecule (DNA or RNA) is the best choice for this test, and whether spirochetes are present in blood during the acute phase of B. miyamotoi disease. A total of 473 patients with a suspected tickborne infection in Yekaterinburg, Russia, in 2009, 2010, and 2015 were enrolled in this study. Blood samples were analyzed by using quantitative PCR or ELISA, and a diagnosis of borreliosis was confirmed for 310 patients. For patients with erythema migrans, 5 (3%) of 167 were positive for B. miyamotoi by PCR; for patients without erythema migrans, 65 (45%) of 143 were positive for B. miyamotoi by PCR. The median concentration for RNA was 3.8 times that for DNA. Median time for detection of B. miyamotoi in blood was 4 days.
Assuntos
Bacteriemia/diagnóstico , Infecções por Borrelia/sangue , Infecções por Borrelia/microbiologia , Borrelia/classificação , Reação em Cadeia da Polimerase/métodos , Bacteriemia/sangue , DNA Bacteriano/sangue , DNA Bacteriano/isolamento & purificação , Eritema Migrans Crônico , Reações Falso-Negativas , Humanos , RNA Bacteriano/sangue , RNA Bacteriano/isolamento & purificação , RNA Ribossômico 16S/genéticaRESUMO
Borrelia miyamotoi sensu lato, a relapsing fever Borrelia sp., is transmitted by the same ticks that transmit B. burgdorferi (the Lyme disease pathogen) and occurs in all Lyme disease-endemic areas of the United States. To determine the seroprevalence of IgG against B. miyamotoi sensu lato in the northeastern United States and assess whether serum from B. miyamotoi sensu lato-infected persons is reactive to B. burgdorferi antigens, we tested archived serum samples from area residents during 1991-2012. Of 639 samples from healthy persons, 25 were positive for B. miyamotoi sensu lato and 60 for B. burgdorferi. Samples from ≈10% of B. miyamotoi sensu lato-seropositive persons without a recent history of Lyme disease were seropositive for B. burgdorferi. Our results suggest that human B. miyamotoi sensu lato infection may be common in southern New England and that B. burgdorferi antibody testing is not an effective surrogate for detecting B. miyamotoi sensu lato infection.
Assuntos
Infecções por Borrelia/epidemiologia , Borrelia/imunologia , Anticorpos Antibacterianos/sangue , Anticorpos Antibacterianos/imunologia , Infecções por Borrelia/sangue , Infecções por Borrelia/imunologia , Feminino , Humanos , Imunoglobulina G/sangue , Imunoglobulina G/imunologia , Doença de Lyme/sangue , Doença de Lyme/epidemiologia , Doença de Lyme/imunologia , Masculino , Pessoa de Meia-Idade , New England/epidemiologia , Estudos SoroepidemiológicosRESUMO
Classifying the states of an individual and quantifying transitions between states are crucial while modeling animal behavior, movement, and physiologic status. When these states are hidden or imperfectly known, it is particularly convenient to relate them to appropriate quantitative measurements taken on the individual. This task is, however, challenging when quantitative measurements are not available at each sampling occasion. For capture-recapture data, various ways of incorporating such non-discrete information have been used, but they are either ad hoc and/or use a fraction of the available information by relying on a priori thresholds to assign individual states. Here we propose assigning discrete states based on a continuous measurement, and then modeled survival and transition probabilities based on these assignments. The main advantage of this new approach is that a more informative use of the non-discrete information is done. As an illustrative working example, we applied this approach to eco-epidemiological data collected across a series of years in which individuals of a long-lived seabird, the Black-legged Kittiwake (Rissa tridactyla), could either be visually detected or physically recaptured and blood sampled for subsequent immunological analyses. We discuss how this approach opens many perspectives in eco-epidemiology, but also more broadly, in population ecology.
Assuntos
Anticorpos Antibacterianos/sangue , Doenças das Aves/sangue , Charadriiformes/sangue , Ecossistema , Animais , Borrelia/imunologia , Infecções por Borrelia/sangue , Infecções por Borrelia/veterinária , Cadeias de Markov , Modelos Biológicos , Dinâmica PopulacionalAssuntos
Infecções por Borrelia/sangue , Infecções por Borrelia/complicações , Borrelia/isolamento & purificação , Febre/sangue , Febre/complicações , Adolescente , Antibacterianos/uso terapêutico , Borrelia/efeitos dos fármacos , Infecções por Borrelia/diagnóstico , Infecções por Borrelia/tratamento farmacológico , Doxiciclina/uso terapêutico , Humanos , RefugiadosRESUMO
Thirty three children with associative forms of thick-borne infection (thick-borne encephalitis with ixodic borreliosis) were clinically observed. The disease was characterized by subfebrile temperature, moderate intoxication, rare erythema (39.5%) and frequent cardiovascular disorders with development of Lyme carditis (32.6 +/- 7.2%) and further rise of hepatomegalia in the diseases dynamics and development of meningeal symptoms. There were observed changes in the cytokine spectrum, characterized by INF-gamma high levels, and hypersecretion of the whole spectrum cytokines in the dynamics, that provided the Th2 type immune response. High clinicoimmunological efficacy of the complex therapy with cycloferon as an immunomodulator providing more balanced production of pro- and anti-inflammatory cytokines (IL-1alpha, INF-gamma and IL-10) was shown.
Assuntos
Acridinas/uso terapêutico , Infecções por Borrelia/tratamento farmacológico , Infecções por Borrelia/etiologia , Encefalite Transmitida por Carrapatos/tratamento farmacológico , Encefalite Transmitida por Carrapatos/etiologia , Indutores de Interferon/uso terapêutico , Interferon gama/sangue , Adolescente , Infecções por Borrelia/sangue , Criança , Pré-Escolar , Citocinas/sangue , Encefalite Transmitida por Carrapatos/sangue , Febre/etiologia , Hepatomegalia , Humanos , Lactente , Interleucina-10/sangue , Interleucina-1alfa/sangue , Células Th2/efeitos dos fármacos , Células Th2/imunologiaRESUMO
In Europe, the hard tick Ixodes ricinus is considered the most important vector of human zoonotic diseases. Human pathogenic agents spread by I. ricinus in Sweden include Borrelia burgdorferi sensu lato (s.l.), Anaplasma phagocytophilum, Rickettsia helvetica, the recently described Neoehrlichia mikurensis, Borrelia miyamotoi, tick-borne encephalitis virus (TBEV), and Babesia spp. (Babesia microti, Babesia venatorum and Babesia divergens). Since these pathogens share the same vector, co-infections with more than one tick-borne pathogen may occur and thus complicate the diagnosis and clinical management of the patient due to possibly altered symptomatology. Borrelia burgdorferi s.l., TBEV and B. miyamotoi are well-known to cause infections of the central nervous system (CNS), whereas the abilities of other tick-borne pathogens to invade the CNS are largely unknown. The aim of this study was to investigate the presence and clinical impact of tick-borne pathogens other than B. burgdorferi s.l. in the cerebrospinal fluid (CSF) and serum samples of patients who were under investigation for Lyme neuroborreliosis (LNB) in a tick-endemic region of South-eastern Sweden. CSF and serum samples from 600 patients, recruited from the Regions of Östergötland County, Jönköping County and Kalmar County in South-eastern Sweden and investigated for LNB during the period of 2009-2013, were retrospectively collected for analysis. The samples were analysed by real-time PCR for the presence of nucleic acid from B. burgdorferi s.l., B. miyamotoi, A. phagocytophilum, Rickettsia spp., N. mikurensis, TBEV and Babesia spp. Serological analyses were conducted in CSF and serum samples for all patients regarding B. burgdorferi s.l., and for the patients with CSF mononuclear pleocytosis, analyses of antibodies to B. miyamotoi, A. phagocytophilum, spotted fever group (SFG) rickettsiae, TBEV and B. microti in serum were performed. The medical charts of all the patients with CSF mononuclear pleocytosis and patients with positive PCR findings were reviewed. Of the 600 patients, 55 (9%) presented with CSF mononuclear pleocytosis, 13 (2%) of whom had Borrelia-specific antibodies in the CSF. One patient was PCR-positive for N. mikurensis, and another one was PCR-positive for Borrelia spp. in serum. No pathogens were detected by PCR in the CSF samples. Four patients had serum antibodies to B. miyamotoi, four patients to A. phagocytophilum, five patients to SFG rickettsiae, and six patients to TBEV. One patient, with antibodies to SFG rickettsiae, had both clinical and laboratory signs suggestive of a current infection. Nine patients had serum antibodies to more than one pathogen, although none of these was assessed as a current co-infection. We can conclude from this study that tick-borne co-infections are uncommon in patients who are being investigated for suspected LNB in South-eastern Sweden, an area endemic for borreliosis and TBE.
Assuntos
Infecções por Borrelia , Coinfecção , Neuroborreliose de Lyme , Anaplasma phagocytophilum/isolamento & purificação , Animais , Borrelia/isolamento & purificação , Infecções por Borrelia/sangue , Infecções por Borrelia/líquido cefalorraquidiano , Borrelia burgdorferi/isolamento & purificação , Vírus da Encefalite Transmitidos por Carrapatos/isolamento & purificação , Humanos , Ixodes/microbiologia , Ixodes/virologia , Neuroborreliose de Lyme/sangue , Neuroborreliose de Lyme/líquido cefalorraquidiano , Neuroborreliose de Lyme/diagnóstico , Patologia Molecular , Reação em Cadeia da Polimerase , Estudos Retrospectivos , Suécia , Doenças Transmitidas por Carrapatos/sangue , Doenças Transmitidas por Carrapatos/líquido cefalorraquidiano , Doenças Transmitidas por Carrapatos/diagnóstico , Zoonoses/complicações , Zoonoses/diagnósticoRESUMO
The agent of African relapsing fever, Borrelia crocidurae, causes reversible multiple organ damage. We hypothesize that this damage is caused when the spirochete forms aggregate with erythrocytes in vivo, creating rosettes that plug the microcirculatory system. To test this hypothesis, we compared testicular microcirculation over an extended time period in two groups of rats: one experimentally inoculated with B. crocidurae, the other with the nonerythrocyte rosette-forming Borrelia hermsii. In the B. crocidurae group, erythrocyte rosettes formed during spiro-chetemia blocked precapillary blood vessels and reduced the normal pattern of microcirculatory blood flow. After spirochetemia, erythrocyte rosettes disappeared and flow was normalized. Decreased blood flow and focal vascular damage with increased permeability and interstitial bleeding adjacent to the erythrocyte microemboli induced cell death in seminiferous tubules. Interestingly, we found that B. crocidurae could penetrate the tubules and remain in the testis long after the end of spirochetemia, suggesting that the testis can serve as a reservoir for this bacteria in subsequent relapses. The group infected with B. hermsii displayed normal testicular blood flow and vasomotion at all selected time points, and suffered no testicular damage. These results confirmed our hypothesis that the erythrocyte rosettes produce vascular obstruction and are the main cause of histopathology seen in model animal and human infections.
Assuntos
Infecções por Borrelia/patologia , Febre Recorrente/patologia , Testículo/lesões , Animais , Barreira Hematotesticular , Borrelia/genética , Borrelia/isolamento & purificação , Borrelia/patogenicidade , Infecções por Borrelia/sangue , Infecções por Borrelia/microbiologia , Morte Celular , Modelos Animais de Doenças , Masculino , Ratos , Ratos Sprague-Dawley , Febre Recorrente/sangue , Febre Recorrente/microbiologia , Túbulos Seminíferos/microbiologia , Túbulos Seminíferos/patologia , Espermatozoides/citologia , Testículo/irrigação sanguínea , Testículo/patologiaRESUMO
BACKGROUND: Borrelia burgdorferi is a tick-borne spirochete that causes Lyme borreliosis (LB). After an initial tick bite, it spreads from the deposition site in the dermis to distant tissues of the host. It is generally believed that this spirochete disseminates via the hematogenous route. Borrelia persica causes relapsing fever and is able to replicate in the blood stream. Currently the exact dissemination pathway of LB pathogens in the host is not known and controversially discussed. METHODS: In this study, we established a strict intravenous infection murine model using host-adapted spirochetes. Survival capacity and infectivity of host-adapted B. burgdorferi sensu stricto (Bbss) were compared to those of B. persica (Bp) after either intradermal (ID) injection into the dorsal skin of immunocompetent mice or strict intravenous (IV) inoculation via the jugular vein. By in vitro culture and PCR, viable spirochetes and their DNA load in peripheral blood were periodically monitored during a 49/50-day course post-injection, as well as in various tissue samples collected at day 49/50. Specific antibodies in individual plasma/serum samples were detected with serological methods. RESULTS: Regardless of ID or IV injection, DNA of Bp was present in blood samples up to day 24 post-challenge, while no Bbss was detectable in the blood circulation during the complete observation period. In contrast to the brain tropism of Bp, Bbss spirochetes were found in ear, skin, joint, bladder, and heart tissue samples of only ID-inoculated mice. All tested tissues collected from IV-challenged mice were negative for traces of Bbss. ELISA testing of serum samples showed that Bp induced gradually increasing antibody levels after ID or IV inoculation, while Bbss did so only after ID injection but not after IV inoculation. CONCLUSIONS: This study allows us to draw the following conclusions: (i) Bp survives in the blood and disseminates to the host's brain via the hematogenous route; and (ii) Bbss, in contrast, is cleared rapidly from the blood stream and is a tissue-bound spirochete.
Assuntos
Infecções por Borrelia/sangue , Infecções por Borrelia/microbiologia , Borrelia burgdorferi/fisiologia , Borrelia/fisiologia , Animais , Infecções por Borrelia/fisiopatologia , Modelos Animais de Doenças , Feminino , Imunocompetência , Injeções Intradérmicas , Injeções Intravenosas , Camundongos , Organismos Livres de Patógenos EspecíficosRESUMO
The hard tick-borne relapsing fever spirochete, Borrelia miyamotoi, has recently gained attention as a cause of human illness, but fundamental aspects of its enzootic maintenance are still poorly understood. Challenges to experimental studies with B. miyamotoi-infected vector ticks include low prevalence of infection in field-collected ticks and seemingly inefficient horizontal transmission from infected immunocompetent rodents to feeding ticks. To reliably produce large numbers of B. miyamotoi-infected ticks in support of experimental studies, we developed an animal model where immunocompromised Mus musculus SCID mice were used as a source of B. miyamotoi-infection for larval and nymphal Ixodes scapularis ticks. Following needle inoculation with 1 × 105 spirochetes, the SCID mice developed a high spirochetemia (greater than 1 × 107 copies of B. miyamotoi purB per mL of blood) that persisted for at least 30 d after inoculation. In comparison, immunocompetent M. musculus CD-1 mice developed transient infections, detectable for only 2-8 d within the first 16 d after needle inoculation, with a brief, lower peak spirochetemia (8.5 × 104 - 5.6 × 105purB copies per mL of blood). All larval or nymphal ticks fed on infected SCID mice acquired B. miyamotoi, but frequent loss of infection during the molt led to the proportion infected ticks of the resulting nymphal or adult stages declining to 22-29%. The ticks that remained infected after the molt had well-disseminated infections which then persisted through successive life stages, including transmission to larval offspring.
Assuntos
Infecções por Borrelia/transmissão , Borrelia , Hospedeiro Imunocomprometido , Ixodes/microbiologia , Febre Recorrente/transmissão , Animais , Bacteriemia , Infecções por Borrelia/sangue , Modelos Animais de Doenças , Feminino , Imunocompetência , Larva/microbiologia , Masculino , Camundongos , Camundongos SCID , Ninfa/microbiologia , Febre Recorrente/sangueRESUMO
The objective of our study was to estimate the seroprevalence of six pathogens transmitted by ticks in HIV-infected persons and blood donors in Poland (B. burgdorferi s.l., A. phagocytophilum, Ehrlichia spp., Babesia spp., Rickettsia spp. Bartonella henselae) to assess the frequency of exposure to such microorganisms in immunocompetent and immunocompromised individuals in endemic regions for I. ricinus ticks. Serum samples were collected from 227 HIV-infected patients and 199 blood donors. All samples were analyzed for antibodies against six tick-borne pathogens and seroprevalence rates were statistically compared between two tested group as well as age, sex and lymphocyte T CD4+ level in HIV infected patients. The seroprevalence of tick-borne infections in HIV-infected patients is higher than that of the healthy population in Poland, although no association between serological status of patients and lymphocyte CD4+ T cell level has been observed. The frequency of tick-borne coinfections and doubtful results of serological tests were significantly higher in HIV-positive individuals. In Poland, the possibility of tick-borne diseases transmission with blood is rather negligible.
Assuntos
Coinfecção/sangue , Coinfecção/epidemiologia , Infecções por HIV/sangue , Infecções por HIV/epidemiologia , HIV/isolamento & purificação , Doenças Transmitidas por Carrapatos/sangue , Doenças Transmitidas por Carrapatos/epidemiologia , Adolescente , Adulto , Idoso , Anaplasma phagocytophilum/isolamento & purificação , Animais , Babesia/isolamento & purificação , Babesiose/sangue , Babesiose/epidemiologia , Babesiose/parasitologia , Doadores de Sangue , Borrelia/isolamento & purificação , Infecções por Borrelia/sangue , Infecções por Borrelia/epidemiologia , Infecções por Borrelia/microbiologia , Estudos de Casos e Controles , Coinfecção/microbiologia , Coinfecção/parasitologia , Ehrlichia/isolamento & purificação , Ehrlichiose/sangue , Ehrlichiose/epidemiologia , Ehrlichiose/microbiologia , Feminino , Infecções por HIV/microbiologia , Infecções por HIV/parasitologia , Humanos , Ixodes/microbiologia , Ixodes/parasitologia , Masculino , Pessoa de Meia-Idade , Polônia/epidemiologia , Prevalência , Estudos Retrospectivos , Rickettsia/isolamento & purificação , Infecções por Rickettsia/sangue , Infecções por Rickettsia/epidemiologia , Infecções por Rickettsia/microbiologia , Estudos Soroepidemiológicos , Infestações por Carrapato , Doenças Transmitidas por Carrapatos/microbiologia , Doenças Transmitidas por Carrapatos/parasitologia , Adulto JovemRESUMO
The plasminogen activation system is part of the fibrinolysis which is tightly regulated and protected against dysfunction by various activators and inhibitors. However, microorganisms including bacteria, fungi and also parasites have been proven to interact in a specific manner with components of the fibrinolytic pathways. Pathogenic bacteria are capable to subvert the function of proteases, activators or inhibitors for their own benefits including dissemination within the host and evasion of host inflammatory immune response. Here, we provide a state of the art overview of the divers strategies employed by bacteria to interact with components of the fibrinolytic system and to exploit the system for invasion. Moreover, the role of factors of the fibrinolytic cascade in inflammatory host response due to different bacterial infections will be presented.
Assuntos
Bactérias/metabolismo , Infecções Bacterianas/sangue , Fibrinólise , Plasminogênio/metabolismo , Sepse/sangue , Animais , Bactérias/enzimologia , Bactérias/patogenicidade , Infecções Bacterianas/enzimologia , Infecções Bacterianas/microbiologia , Proteínas de Bactérias/metabolismo , Infecções por Borrelia/sangue , Infecções por Borrelia/enzimologia , Infecções por Borrelia/microbiologia , Proteínas de Transporte/metabolismo , Modelos Animais de Doenças , Helicobacter pylori/metabolismo , Humanos , Meningites Bacterianas/sangue , Meningites Bacterianas/enzimologia , Meningites Bacterianas/microbiologia , Metaloendopeptidases/metabolismo , Camundongos , Inibidor 1 de Ativador de Plasminogênio/metabolismo , Ativadores de Plasminogênio/metabolismo , Pneumonia Bacteriana/sangue , Pneumonia Bacteriana/enzimologia , Pneumonia Bacteriana/microbiologia , Receptores de Superfície Celular/metabolismo , Receptores de Ativador de Plasminogênio Tipo Uroquinase , Sepse/enzimologia , Sepse/microbiologia , Transdução de Sinais , Estreptoquinase/metabolismo , Ativador de Plasminogênio Tecidual/metabolismo , Ativador de Plasminogênio Tipo Uroquinase/metabolismoRESUMO
The biochemical parameters of hepatobiliary system functions were studied in patients with opisthorchiasis and concomitant diseases, such as chronic viral hepatitis concurrent with chronic opisthorchiasis, as well as Ixodes tick-borne borreliosis in the presence of the same invasion. Although the magnitude ofbiochemical changes is not great in chronic opisthorchiasis or chronic viral hepatitis, the concomitance of these two diseases were ascertained to result in pronounced abnormalities, by demonstrating the exhaustion of spare capacities of the hepatobiliary system in parasitic invasion (or viral infection). When opisthorchiasis was concurrent with Ixodes tickborne borreliosis, some parameters under study differed from those in the groups of patients with monoinfections. Variance analysis showed that chronic opisthorchiasis had a great impact on carbohydrate and lipid metabolisms (glucose and cholesterol levels). The findings suggest that the formation of stable host-parasite relationships in chronic opisthorchiasis alters human metabolic processes and their compensatory capabilities.
Assuntos
Infecções por Borrelia/sangue , Infecções por Borrelia/complicações , Borrelia burgdorferi , Hepatite Viral Humana/sangue , Hepatite Viral Humana/complicações , Opistorquíase/sangue , Opistorquíase/complicações , Opisthorchis , Alanina Transaminase/sangue , Animais , Sistema Biliar/fisiopatologia , Bilirrubina/sangue , Infecções por Borrelia/fisiopatologia , Colesterol/sangue , Colinesterases/sangue , Doença Crônica , Glucose/análise , Glutamil Aminopeptidase/sangue , Hepatite Viral Humana/fisiopatologia , Humanos , Fígado/fisiopatologia , Opistorquíase/parasitologia , Opistorquíase/fisiopatologia , Opisthorchis/fisiologia , gama-Glutamiltransferase/sangueRESUMO
BACKGROUND: Borrelia miyamotoi causes systemic febrile illness and is transmitted by the same tick species that transmits Borrelia burgdorferi sensu lato and tick-borne encephalitis virus. We describe a serological test using a fragment of glycerophosphodiester phosphodiesterase (GlpQ) as an antigen, and determined its performance in well-defined patient categories. METHODS: Serum of patients with PCR-confirmed Borrelia miyamotoi disease (BMD), Lyme borreliosis (LB), tick-borne encephalitis (TBE), and healthy blood donors (HBD) were collected in Udmurt Republic, Russia. Sera of BMD and LB patients were collected at hospital admission, one week, one month and one year after admission. RESULTS: The levels of IgM and IgG anti-GlpQ antibodies, determined as optical density values in Luminex bead-based assays, were significantly higher in the BMD patient group than in LB patients, TBE patients or HBD group (all p<0.05). CONCLUSIONS: By using a strict cut-off value, it was possible to exclude B. miyamotoi infection in LB and TBE patients and to serologically confirm B. miyamotoi infection in 44% to 94% of the PCR-positive BMD patients (95% confidence interval). Thus, sensitive serological assays should not solely rely on rGlpQ, to support the diagnosis of acute BMD.
Assuntos
Infecções por Borrelia/diagnóstico , Infecções por Borrelia/imunologia , Borrelia/imunologia , Borrelia/patogenicidade , Testes Sorológicos/métodos , Adolescente , Adulto , Anticorpos/sangue , Anticorpos Antibacterianos/sangue , Anticorpos Antibacterianos/imunologia , Borrelia/enzimologia , Infecções por Borrelia/sangue , Coinfecção , DNA Bacteriano/sangue , Vírus da Encefalite Transmitidos por Carrapatos/patogenicidade , Encefalite Transmitida por Carrapatos/sangue , Encefalite Transmitida por Carrapatos/diagnóstico , Europa (Continente) , Feminino , Humanos , Imunoglobulina G/sangue , Imunoglobulina M/sangue , Doença de Lyme/sangue , Doença de Lyme/diagnóstico , Masculino , Pessoa de Meia-Idade , Diester Fosfórico Hidrolases/análise , Diester Fosfórico Hidrolases/imunologia , Reação em Cadeia da Polimerase/métodos , Federação Russa , Sensibilidade e Especificidade , Adulto JovemAssuntos
Infecções por Borrelia/fisiopatologia , Borrelia/crescimento & desenvolvimento , Doxiciclina/uso terapêutico , Ornithodoros/microbiologia , Febre Recorrente/fisiopatologia , Spirochaetales/crescimento & desenvolvimento , Adulto , Animais , Antibacterianos/administração & dosagem , Antibacterianos/uso terapêutico , Vetores Artrópodes/microbiologia , Buffy Coat/microbiologia , Borrelia/efeitos dos fármacos , Infecções por Borrelia/sangue , Infecções por Borrelia/complicações , Infecções por Borrelia/tratamento farmacológico , Doxiciclina/administração & dosagem , Humanos , Masculino , RNA Ribossômico 16S/análise , Febre Recorrente/sangue , Febre Recorrente/tratamento farmacológico , Febre Recorrente/etiologia , Análise de Sequência de DNA , Spirochaetales/efeitos dos fármacos , Tadjiquistão , UzbequistãoRESUMO
Malaria and relapsing fever are arthropod-borne infections characterized by fever, myalgia, headache, and a tendency to relapse. Both are diagnosed through examination of stained blood films, and both might respond to tetracycline therapy. In at least four published case reports, the presence of malarial microgametes possibly resulted in misdiagnosis of borreliosis in patients with malaria. An additional case is presented, and the mechanism of microgamete production in clinical specimens is discussed.
Assuntos
Infecções por Borrelia/diagnóstico , Malária Vivax/sangue , Malária Vivax/diagnóstico , Adulto , Antimaláricos/uso terapêutico , Infecções por Borrelia/sangue , Infecções por Borrelia/complicações , Cloroquina/uso terapêutico , Erros de Diagnóstico , Feminino , Humanos , Índia , Israel , Malária Vivax/tratamento farmacológico , Primaquina/uso terapêutico , Viagem , Resultado do TratamentoRESUMO
Elucidation of the pathophysiological basis of platelet disorders in murine models requires a reliable method for the frequent determinations of platelet counts in individual mice. Here, we present a rapid, reproducible and accurate flow cytometric method for enumeration of platelets that involves fluorescent staining of platelets in whole blood with specific antibody and the addition of known numbers of fluorescent beads for standardization of the sample volume. Analysis of platelets obtained by tail bleeding indicated that this sampling procedure did not activate platelets, and that only five microliters of blood were required for platelet counting. Using this method, we followed platelet counts in mice infected with the relapsing fever spirochete Borrelia turicatae for 26 days, and found that this bacterium induces thrombocytopenia, a common manifestation of human relapsing fever. Therefore, this method can be used to follow the number and the activation state of circulating platelets from individual mice over extended periods of time and is applicable to a wide range of murine models of platelet disorders.
Assuntos
Plaquetas/citologia , Contagem de Plaquetas/métodos , Animais , Anticorpos Monoclonais , Antígenos CD/análise , Plaquetas/imunologia , Plaquetas/fisiologia , Infecções por Borrelia/sangue , Infecções por Borrelia/complicações , Modelos Animais de Doenças , Citometria de Fluxo/normas , Imunofluorescência , Integrina beta3 , Camundongos , Camundongos Endogâmicos C57BL , Selectina-P/análise , Ativação Plaquetária , Glicoproteínas da Membrana de Plaquetas/análise , Padrões de Referência , Trombocitopenia/diagnóstico , Trombocitopenia/etiologiaRESUMO
In spite of significant advances in immunologically based testing, accurate diagnosis of Lyme borreliosis remains problematic. To address this issue, a DNA amplification-based diagnostic test was developed utilizing the polymerase chain reaction (PCR) and oligonucleotide primers specific for the OspA and OspB genes of Borrelia burgdorferi. In this approach, a relatively large DNA fragment is amplified with an outer set of primers, and a "nested" internal sequence of the PCR product subsequently reamplified with an inner set of primers. This nested approach coupled with simple differential centrifugation allowed specific detection of as few as four B. burgdorferi organisms mixed in 2 ml of blood. This methodology was utilized on patients' samples, and it allowed detection of B. burgdorferi in the peripheral blood and urine of several individuals with clinical evidence of Lyme borreliosis. PCR became negative and symptoms improved following antibiotic therapy of treated individuals. These studies suggest that direct detection of Borrelia in infected individuals can aid in diagnosis and evaluation of therapy for Lyme borreliosis.
Assuntos
Infecções por Borrelia/diagnóstico , Grupo Borrelia Burgdorferi/isolamento & purificação , Reação em Cadeia da Polimerase , Sequência de Bases , Infecções por Borrelia/sangue , Infecções por Borrelia/microbiologia , Infecções por Borrelia/urina , Grupo Borrelia Burgdorferi/genética , Centrifugação , Criança , DNA Bacteriano/análise , Humanos , Masculino , Pessoa de Meia-Idade , Dados de Sequência Molecular , Alinhamento de SequênciaRESUMO
A low-passage, Portuguese isolate of Borrelia lusitaniae, strain PotiB2, was inoculated into C3H/HeN mice and disease was monitored by histopathology at 8 weeks after spirochaete challenge. Ear, heart, bladder, femoro-tibial joint, brain and spinal cord were examined. B. lusitaniae strain PotiB2 (6 of 10 mice) and B. burgdorferi sensu stricto strain N40 (9 of 10 mice) induced similar lesions in the bladder of infected mice characterised as a multifocal, lymphoid, interstitial cystitis. Moreover, both B. lusitaniae PotiB2 and B. burdorferi N40 induced lesions in the heart of infected mice. The lesions induced by B. lusitaniae PotiB2 (2 of 10 mice) were characterised as a severe, necrotising endarteritis of the aorta, with a minimal, mixed inflammatory infiltrate (neutrophils, macrophages and lymphoid cells) extending into the adjacent myocardium. In contrast, B. burgdorferi N40 induced a periarteritis of the pulmonary artery (7 of 10 mice), with no involvement of the endothelium and more extensive inflammation and subsequent necrosis of the adjacent myocardium. This infiltrate was composed entirely of mononuclear cells, predominantly mature lymphocytes and plasma cells. No lesions were noted in the joints or central nervous system with inoculation of strains N40 or PotiB2, and co-inoculation of either strain with Ixodes ricinus salivary gland lysate did not affect the resulting pathology. Serology, examined 8 weeks after inoculation, indicated a different reactivity in mice infected with B. lusitaniae PotiB2 compared with B. burgdorferi N40. Immunoblot analysis demonstrated that mice with lesions resulting from infection with B. lusitaniae PotiB2 reacted only to the flagellin protein (41 kDa) or to flagellin and OspC, whereas mice infected with B. burgdorferi N40 reacted with multiple high and low mol. wt proteins, including flagellin, p93, p39, OspA, OspB and OspC. These results indicate that B. lusitaniae PotiB2 induced pathology similar to B. burgdorferi N40 when inoculated into susceptible mice. Moreover, these results establish the first animal model of disease with B. lusitaniae. This mouse model can be used to characterise the immunopathogenesis of B. lusitaniae infection and to delineate the proteins responsible for disease induction in susceptible mice.
Assuntos
Antígenos de Bactérias , Infecções por Borrelia/patologia , Borrelia/patogenicidade , Animais , Proteínas da Membrana Bacteriana Externa/imunologia , Proteínas da Membrana Bacteriana Externa/metabolismo , Borrelia/isolamento & purificação , Infecções por Borrelia/sangue , Infecções por Borrelia/microbiologia , Borrelia burgdorferi/patogenicidade , Encéfalo/microbiologia , Encéfalo/patologia , Cistite Intersticial , Modelos Animais de Doenças , Suscetibilidade a Doenças , Orelha/microbiologia , Orelha/patologia , Flagelina , Coração/microbiologia , Immunoblotting , Imuno-Histoquímica , Ixodes/microbiologia , Camundongos , Camundongos Endogâmicos C3H , Miocárdio/patologia , Portugal , Medula Espinal/microbiologia , Medula Espinal/patologia , Bexiga Urinária/microbiologia , Bexiga Urinária/patologiaRESUMO
Inbred C57bl/6 mice were challenged with high-passage Borrelia afzelii, Borrelia garinii and Borrelia burgdorferi sensu stricto and tested for antigen specific T-cell response in vitro. Sonicated preparations of washed spirochetes were potent cell activators, capable of stimulating polyclonal proliferation after 72h of culture while increasing the incubation time up to 120h provoked specific cell-mediated response. Isolated murine spleocytes previously sensitized to B. burgdorferi sensu lato but not those from control mice could be induced for antigen-specific proliferation in vitro, as revealed by [3H]thymidine incorporation assay, Moreover, in mice presensitized to B. burgdorferi sensu lato, detectable cell-mediated response could be induced only with antigen preparations derived from a corresponding strain but not with those obtained from other Borrelia genospecies. The current study emphasises that the B. burgdorferi antigen-specific response may also be expected in different genospecies infections in men.