Abstract We
report an
Acanthamoeba keratitis case associated with the use of
contact lens in a 28-year-old
female from Brasília,
Brazil. Samples from corneal scraping and
contact lens case were used for
culture establishment,
PCR amplification, and partial sequencing (fragments of ~400kb) of small subunit
rDNA; both
culture and
PCR were positive. The
sequence analyses of the
cornea and of isolates from the
contact lens case showed similarity with the T4
genotype. To the best of our
knowledge, this is the first
report of T4
Acanthamoeba keratitis case from the Midwest region of
Brazil.