Callithrix penicillata: a feasible experimental model for dengue virus infection
Ferreira, Milene Silveira; Castro, Paulo Henrique Gomes de; Silva, Gilmara Abreu; Casseb, Samir Mansur Moraes; Dias Júnior, Antônio Gregório; Rodrigues, Sueli Guerreiro; Azevedo, Raimunda do Socorro da Silva; Silva, Matheus Fernandes Costa e; Zauli, Danielle Alves Gomes; Araújo, Márcio Sobreira Silva; Béla, Samantha Ribeiro; Teixeira Carvalho, Andréa; Martins Filho, Olindo Assis; Vasconcelos, Pedro Fernando da Costa.
Immunol. lett
; 158(1-2): 126 - 133, 2014. ilus
Artigo
em Inglês
| Instituto Evandro Chagas (SOPHIA) | ID: iec-13984
Although the murine models have the feasibility to reproduce some signs of dengue Virus (DENV) infection, the use of isogenic hosts with polarized immune response patterns does not reproduce the particularities of human disease. Our goal was to investigate the kinetics of peripheral blood biomarkers in immunocompetent Callithrix penicillata non-human primates subcutaneously infected with DENV-3. The viral load of infected animals was determinated by quantitative real time PCR. Measurements of DENV-3/IgM were performed, and several parameters were assessed by hemogram red blood cells count, hemoglobin, hematocrit, white blood cells count, neutrophils, monocytes, lymphocytes, and platelets count. The coagulogram was performed by prothrombin time (PT), and activated partial thromboplastin time (APTT) assays. The renal function was monitored by urea and creatinine, and the liver function by the aspartate (AST), and alanine (ALT) aminotransferases. Also, the level of the cytokines IL-6, TNF-, IL-2, IFN-, IL-4 and IL-5 was quantified during the experimental study. Data analysis was performed considering relevant differences when baseline fold changes were found outside from 0.75 to 1.5 range. (AU)
Biblioteca responsável:
BR275.1
Localização: PCIEC2014 / BR275.1
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