LIMA, Alex Ranieri Jerônimo et al. Insights into Limnothrix sp. metabolism based on comparative genomics. Frontiers in Microbiology, v.9, n. 2811, Nov. 2018. DOI: https://doi.org/10.3389/fmicb.2018.02811. Disponível em: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6256058/pdf/fmicb-09-02811.pdf.
Artigo
em Inglês
| Instituto Evandro Chagas (DSpace) | ID: ied-3836
Currently only four genome sequences for Limnothrix spp. are publicly available, and information on the genetic properties of cyanobacteriabelonging to this genus is limited. In this study, we report the draft genome of Limnothrix sp. CACIAM 69d, isolated from the reservoir of a hydroelectric dam located in the Amazonecosystem, from where cyanobacterial genomic data are still scarce. Comparative genomicanalysis of Limnothrix revealed the presence of key enzymes in the cyanobacterial central carbonmetabolism and how it is well equipped for environmental sulfur and nitrogen acquisition. Additionally, this work covered the analysis of Limnothrix CRISPR-Cas systems, pathways related to biosynthesis of secondary metabolites and assembly of extracellular polymeric substances and their exportation. A trans-AT PKS gene cluster was identified in two strains, possibly related to the novel toxin Limnothrixin biosynthesis. Overall, the draft genome of Limnothrix sp. CACIAM 69d adds new data to the small Limnothrix genome library and contributes to a growing representativeness of cyanobacterial genomes from the Amazon region. The comparative genomicanalysis of Limnothrix made it possible to highlight unique genes for each strain and understand the overall features of their metabolism. / This work was funded by Brazilian Conselho Nacional de Desenvolvimento Científico e Tecnológico CNPq (grants 556851/2009-9 and 554321/2010-6) and Fundação Amazônia Paraense (FAPESPA) (grant ICAAF 006/2012). This work was financially supported through grants from CAPES (Coordination for the Improvement of Higher EducationPersonnel, Brazil), PROPESP/UFPA (Pró-Reitoria de Pesquisa e Pós-Graduação/Universidade Federal do Pará, Brazil), FAPEMA (Maranhão StateResearchFoundation, Brazil), and Instituto Evandro Chagas (IEC).