Characterization of mitochondrial iron uptake in HepG2 cells
Biol. Res
; 39(1): 199-201, 2006. ilus
Article
em En
| LILACS
| ID: lil-430715
Biblioteca responsável:
BR1.1
RESUMO
There is increasing evidence that accumulation of redox-active iron in mitochondria leads to oxidative damage and contributes to various neurodegenerative diseases, such as Friedreich's ataxia and Parkinson's disease. In this work, we examined the existence of regulatory mechanisms for mitochondrial iron uptake and storage. To that end, we used rhodamine B- [(1,10-phenanthrolin-5-yl)amino carbonyl ] benzyl ester, a new fluorescent iron-sensitive probe that is targeted specifically to the mitochondrion. We found that extracellular iron was incorporated readily into mitochondria in an apparently saturable process. Moreover, the rate of iron incorporation responded to the Fe status of the cell, an indication that the mitochondrion actively regulates its iron content.
Texto completo:
1
Base de dados:
LILACS
Assunto principal:
Rodaminas
/
Corantes Fluorescentes
/
Ferro
/
Mitocôndrias
Limite:
Humans
Idioma:
En
Revista:
Biol. Res
Assunto da revista:
BIOLOGIA
Ano de publicação:
2006
Tipo de documento:
Article
/
Project document
País de afiliação:
Chile
/
Israel