Serological evidence for Saint Louis encephalitis virus in free-ranging New World monkeys and horses within the upper Paraná River basin region, Southern Brazil
Svoboda, Walfrido Kühl; Martins, Lívia Carício; Malanski, Luciano de Souza; Shiozawa, Marcos Massaaki; Spohr, Kledir Anderson Hofstaetter; Hilst, Carmen Lúcia Scortecci; Aguiar, Lucas M.; Ludwig, Gabriela; Passos, Fernando de Camargo; Silva, Lineu Roberto da; Headley, Selwyn Arlington; Navarro, Italmar Teodorico.
Rev. Soc. Bras. Med. Trop
; 47(3): 280-286, May-Jun/2014. tab, graf
Artigo
em Inglês
| LILACS | ID: lil-716403
Introduction Saint Louis encephalitis virus (SLEV) primarily occurs in the Americas and produces disease predominantly in humans. This study investigated the serological presence of SLEV in nonhuman primates and horses from southern Brazil. Methods From June 2004 to December 2005, sera from 133 monkeys (Alouatta caraya, n=43; Sapajus nigritus, n=64; Sapajus cay, n=26) trap-captured at the Paraná River basin region and 23 blood samples from farm horses were obtained and used for the serological detection of a panel of 19 arboviruses. All samples were analyzed in a hemagglutination inhibition (HI) assay; positive monkey samples were confirmed in a mouse neutralization test (MNT). Additionally, all blood samples were inoculated into C6/36 cell culture for viral isolation. Results Positive seroreactivity was only observed for SLEV. A prevalence of SLEV antibodies in sera was detected in Alouatta caraya (11.6%; 5/43), Sapajus nigritus (12.5%; 8/64), and S. cay (30.8%; 8/26) monkeys with the HI assay. Of the monkeys, 2.3% (1/42) of A. caraya, 6.3% 94/64) of S. nigritus, and 15.4% (4/26) of S. cay were positive for SLEV in the MNT. Additionally, SLEV antibodies were detected by HI in 39.1% (9/23) of the horses evaluated in this study. Arboviruses were not isolated from any blood sample. Conclusions These results confirmed the presence of SLEV in nonhuman primates and horses from southern Brazil. These findings most likely represent the first detection of this virus in nonhuman primates beyond the Amazon region. The detection of SLEV in animals within a geographical region distant from the Amazon basin suggests that there may be widespread and undiagnosed dissemination of this disease in Brazil. .
Assuntos
Animais Camundongos Vírus da Encefalite de St. Louis/imunologia Encefalite de St. Louis/veterinária Doenças dos Cavalos/epidemiologia Doenças dos Macacos/epidemiologia Anticorpos Antivirais/sangue Brasil/epidemiologia Encefalite de St. Louis/diagnóstico Encefalite de St. Louis/epidemiologia Cavalos Testes de Inibição da Hemaglutinação/veterinária Doenças dos Cavalos/diagnóstico Doenças dos Cavalos/virologia Doenças dos Macacos/diagnóstico Doenças dos Macacos/virologia Platirrinos Prevalência
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