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Evaluation of four molecular methods for the diagnosis of tuberculosis in pulmonary and blood samples from immunocompromised patients
Lyra, Juliana Maria Azevedo de; Maruza, Magda; Verza, Mirela; Carneiro, Maria Madileuza; Albuquerque, Maria de Fátima Militão de; Rossetti, Maria Lúcia; Ximenes, Ricardo; Braga, Maria Cynthia; Lucena-Silva, Norma.
Afiliação
  • Lyra, Juliana Maria Azevedo de; Centro de Pesquisas Aggeu Magalhães-Fiocruz. Departamento de Imunologia. Recife. BR
  • Maruza, Magda; Centro de Pesquisas Aggeu Magalhães-Fiocruz. Departamento de Imunologia. Recife. BR
  • Verza, Mirela; Centro de Pesquisas Aggeu Magalhães-Fiocruz. Departamento de Imunologia. Recife. BR
  • Carneiro, Maria Madileuza; Centro de Pesquisas Aggeu Magalhães-Fiocruz. Departamento de Imunologia. Recife. BR
  • Albuquerque, Maria de Fátima Militão de; Centro de Pesquisas Aggeu Magalhães-Fiocruz. Departamento de Imunologia. Recife. BR
  • Rossetti, Maria Lúcia; Centro de Pesquisas Aggeu Magalhães-Fiocruz. Departamento de Imunologia. Recife. BR
  • Ximenes, Ricardo; Centro de Pesquisas Aggeu Magalhães-Fiocruz. Departamento de Imunologia. Recife. BR
  • Braga, Maria Cynthia; Centro de Pesquisas Aggeu Magalhães-Fiocruz. Departamento de Imunologia. Recife. BR
  • Lucena-Silva, Norma; Centro de Pesquisas Aggeu Magalhães-Fiocruz. Departamento de Imunologia. Recife. BR
Mem. Inst. Oswaldo Cruz ; 109(6): 805-813, 09/09/2014. tab, graf
Article em En | LILACS | ID: lil-723984
Biblioteca responsável: BR1.1
ABSTRACT
The present study analysed the concordance among four different molecular diagnostic methods for tuberculosis (TB) in pulmonary and blood samples from immunocompromised patients. A total of 165 blood and 194 sputum samples were collected from 181 human immunodeficiency virus (HIV)-infected patients with upper respiratory complaints, regardless of suspicious for TB. The samples were submitted for smear microscopy, culture and molecular tests a laboratory-developed conventional polymerase chain reaction (PCR) and real-time quantitative PCR (qPCR) and the Gen-Probe and Detect-TB Ampligenix kits. The samples were handled blindly by all the technicians involved, from sample processing to results analysis. For sputum, the sensitivity and specificity were 100% and 96.7% for qPCR, 81.8% and 94.5% for Gen-Probe and 100% and 66.3% for Detect-TB, respectively. qPCR presented the best concordance with sputum culture [kappa (k) = 0.864)], followed by Gen-Probe (k = 0.682). For blood samples, qPCR showed 100% sensitivity and 92.3% specificity, with a substantial correlation with sputum culture (k = 0.754) and with the qPCR results obtained from sputum of the corresponding patient (k = 0.630). Conventional PCR demonstrated the worst results for sputa and blood, with a sensitivity of 100% vs. 88.9% and a specificity of 46.3% vs. 32%, respectively. Commercial or laboratory-developed molecular assays can overcome the difficulties in the diagnosis of TB in paucibacillary patients using conventional methods available in most laboratories.
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Texto completo: 1 Base de dados: LILACS Assunto principal: Escarro / Tuberculose Pulmonar / Infecções por HIV / Hospedeiro Imunocomprometido / Técnicas de Diagnóstico Molecular / Mycobacterium tuberculosis Tipo de estudo: Diagnostic_studies / Evaluation_studies Limite: Humans Idioma: En Revista: Mem. Inst. Oswaldo Cruz Assunto da revista: MEDICINA TROPICAL / PARASITOLOGIA Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Brasil

Texto completo: 1 Base de dados: LILACS Assunto principal: Escarro / Tuberculose Pulmonar / Infecções por HIV / Hospedeiro Imunocomprometido / Técnicas de Diagnóstico Molecular / Mycobacterium tuberculosis Tipo de estudo: Diagnostic_studies / Evaluation_studies Limite: Humans Idioma: En Revista: Mem. Inst. Oswaldo Cruz Assunto da revista: MEDICINA TROPICAL / PARASITOLOGIA Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Brasil