Your browser doesn't support javascript.
loading
Effects of metoclopramide on the expression of metalloproteinases and interleukins in left colonic anastomoses. An experimental study
Silva, Silvana Marques e; Jerônimo, Márcio Sousa; Silva-Pereira, Ildinete da; Tavares, Aldo Henrique; Bocca, Anamélia Lorenzetti; Sousa, João Batista de.
Afiliação
  • Silva, Silvana Marques e; Universidade de Brasilia. School of Medicine. BR
  • Jerônimo, Márcio Sousa; Universidade de Brasilia. School of Medicine. BR
  • Silva-Pereira, Ildinete da; Universidade de Brasilia. School of Medicine. BR
  • Tavares, Aldo Henrique; Universidade de Brasilia. School of Medicine. BR
  • Bocca, Anamélia Lorenzetti; Universidade de Brasilia. School of Medicine. BR
  • Sousa, João Batista de; Universidade de Brasilia. School of Medicine. BR
Acta cir. bras ; Acta cir. bras;30(11): 762-769, Nov. 2015. tab, graf
Article em En | LILACS | ID: lil-767600
Biblioteca responsável: BR1.1
ABSTRACT

PURPOSE:

To evaluate the effects of metoclopramide on metalloproteinases (MMP) and interleukins (IL) gene expression in colonic anastomoses in rats.

METHODS:

Eighty rats were divided into two groups for euthanasia on the 3rd or 7th postoperative day (POD), then into two subgroups for sepsis induction or not, and then into subgroups to receive either metoclopramide or saline solution. Left colonic anastomosis were performed and then analyzed.

RESULTS:

On the 3rd POD, metoclopramide was associated with increased expression of MMP-1a, MMP-13, and TNF-α. On the 7th POD, the transcripts of all MMPs, TNF-α, IL-1β, IFN-γ, and IL-10 of the treated animals became negatively modulated. In the presence of sepsis, metoclopramide did not change MMPs and decreased IL-6, IL-1β, IFN-γ and IL-10 gene expression on the 3rd POD. On the 7th POD, increased expression of all MMPs, IFN-γ and IL-10 and negative modulated TNF-α and IL-6 gene expression.

CONCLUSION:

Administration of metoclopramide increased metalloproteinases and interleukins gene expression on the 3rd postoperative day and negatively modulated them on the 7th POD. In the presence of abdominal sepsis, metoclopramide did not change MMPs and decreased ILs gene expression on the 3rd POD. On the 7th POD, the drug increased expression of all MMPs.
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: LILACS Assunto principal: Expressão Gênica / Interleucinas / Colo / Metaloproteases / Metoclopramida / Antieméticos Tipo de estudo: Etiology_studies / Evaluation_studies Limite: Animals Idioma: En Revista: Acta cir. bras Assunto da revista: Cirurgia Geral / Procedimentos Cir£rgicos Operat¢rios Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Brasil

Texto completo: 1 Base de dados: LILACS Assunto principal: Expressão Gênica / Interleucinas / Colo / Metaloproteases / Metoclopramida / Antieméticos Tipo de estudo: Etiology_studies / Evaluation_studies Limite: Animals Idioma: En Revista: Acta cir. bras Assunto da revista: Cirurgia Geral / Procedimentos Cir£rgicos Operat¢rios Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Brasil