WRM-1 activates the LIT-1 protein kinase to transduce anterior/posterior polarity signals in C. elegans.
Cell
; 97(6): 717-26, 1999 Jun 11.
Article
em En
| MEDLINE
| ID: mdl-10380924
ABSTRACT
During C. elegans development, Wnt/WG signaling is required for differences in cell fate between sister cells born from anterior/posterior divisions. A beta-catenin-related gene, wrm-1, and the lit-1 gene are effectors of this signaling pathway and appear to downregulate the activity of POP-1, a TCF/LEF-related protein, in posterior daughter cells. We show here that lit-1 encodes a serine/threonine protein kinase homolog related to the Drosophila tissue polarity protein Nemo. We demonstrate that the WRM-1 protein binds to LIT-1 in vivo and that WRM-1 can activate the LIT-1 protein kinase when coexpressed in vertebrate tissue culture cells. This activation leads to phosphorylation of POP-1 and to apparent changes in its subcellular localization. Our findings provide evidence for novel regulatory avenues for an evolutionarily conserved Wnt/WG signaling pathway.
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Base de dados:
MEDLINE
Assunto principal:
Transdução de Sinais
/
Proteínas de Helminto
/
Transativadores
/
Proteínas Quinases Ativadas por Mitógeno
/
Proteínas do Citoesqueleto
/
Proteínas de Caenorhabditis elegans
/
Proteínas de Membrana
Limite:
Animals
Idioma:
En
Revista:
Cell
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
Estados Unidos