Amplification of cyclin D1 and MDM-2 in oesophageal carcinoma.
Eur J Surg Oncol
; 25(4): 364-7, 1999 Aug.
Article
em En
| MEDLINE
| ID: mdl-10419705
ABSTRACT
AIMS:
This study investigated amplification of the cyclin D1 and MDM-2 genes, and overexpression of the cyclin D1 gene product, in oesophageal carcinoma.METHODS:
Paired tumour and normal DNA samples from 26 oesophageal adenocarcinomas and 19 squamous cell carcinomas were analysed by Southern blotting with specific DNA probes for cyclin D1 and MDM-2, and for a control gene (alpha-lactalbumin). The cyclin D1 and MDM-2 gene copy numbers were calculated for each tumour. Expression of the cyclin D1 gene was assessed by immunohistochemical analysis of its protein product.RESULTS:
Cyclin D1 gene amplification (by a factor of between two and six) was identified in seven tumours (16%). MDM-2 gene amplification (by a factor of between two and 11) was identified in 10 tumours (22%). Overexpression of cyclin D1 protein was identified in eight tumours and was significantly associated with gene amplification (P=0.04; Fisher's exact test), and with early T stage (P=0.01; Fisher's exact test).CONCLUSIONS:
Cyclin D1 and MDM-2 amplification and cyclin D1 overexpression occur, although infrequently, in the development of oesophageal carcinoma. Cyclin D1 overexpression may influence tumour behaviour, causing the disease to present at an earlier T stage. The mechanism for this effect is unclear, and warrants further investigation.
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias Esofágicas
/
Proteínas Nucleares
/
Carcinoma de Células Escamosas
/
Adenocarcinoma
/
Amplificação de Genes
/
Proteínas Proto-Oncogênicas
/
Ciclina D1
/
Proteínas de Neoplasias
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Eur J Surg Oncol
Assunto da revista:
NEOPLASIAS
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
Reino Unido