Integrated stimulation by CXC chemokines enhances PMN [Ca2+]i signaling in trauma and adult respiratory distress syndrome.
Surgery
; 126(2): 208-15, 1999 Aug.
Article
em En
| MEDLINE
| ID: mdl-10455886
ABSTRACT
BACKGROUND:
Trauma and adult respiratory distress syndrome (ARDS) are associated with increased CXC chemokine (CXC) activity. CXCs such as interleukin (IL)-8 activate polymorphonuclear neutrophils (PMNs) in the lung by means of calcium signals ([Ca2+]i). We studied CXC effects on PMN [Ca2+]i in ARDS and trauma.METHODS:
Isolated PMNs were loaded with Fura-2 dye. Normal PMNs were incubated in ARDS plasma or volunteer plasma, with or without blocking antibodies to IL-8, growth-related oncogene alpha (GRO-alpha), or both (n = 6 pairs), and then stimulated with 1 to 10 nmol/L IL-8. PMNs from trauma patients or volunteers (n = 10 pairs) were stimulated with GRO-alpha, or with sequential GRO-alpha/IL-8. [Ca2+]i was measured with spectrofluorometry.RESULTS:
[Ca2+]i responses to IL-8 were higher after being incubated in ARDS plasma than in volunteer plasma (251 +/- 33 vs 218 +/- 33 nmol/L, P = .03). Blockade of GRO-alpha or IL-8 reversed ARDS plasma effects. After GRO-alpha/IL-8, PMNs from trauma patients demonstrated more Ca2+ store release than did PMNs from volunteers (235 +/- 13 vs 170 +/- 10 nmol/L, P < .01). Conversely, PMNs from trauma patients lost receptor-operated Ca2+ influex to GRO-alpha.CONCLUSIONS:
In traumatic ARDS, plasma CXCs prime PMNs for higher [Ca2+]i flux, making PMN activation more likely. IL-8 and GRO-alpha interact to modulate these PMN [Ca2+]i responses.
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Base de dados:
MEDLINE
Assunto principal:
Síndrome do Desconforto Respiratório
/
Ferimentos e Lesões
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Fatores Quimiotáticos
/
Interleucina-8
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Substâncias de Crescimento
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Quimiocinas CXC
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Sinalização do Cálcio
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Peptídeos e Proteínas de Sinalização Intercelular
/
Neutrófilos
Limite:
Adult
/
Humans
/
Male
Idioma:
En
Revista:
Surgery
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
Estados Unidos