T-cell development in human thymoma.
Pathol Res Pract
; 195(8): 541-7, 1999.
Article
em En
| MEDLINE
| ID: mdl-10483584
ABSTRACT
Human thymoma is derived from thymic epithelial cells and often associated with a large number of cortical thymocytes. Since thymic epithelial cells play key roles in T-cell development in the normal thymus, we hypothesized that the neoplastic epithelial cells of thymoma may support T-cell differentiation. We attempted to reconstitute the T-cell development in vitro by using neoplastic epithelial cells isolated from thymoma. CD34, a stem cell marker, was expressed on a proportion of CD4-CD8- cells in thymoma. These CD34+CD4-CD8- cells also expressed both IL-7R alpha-chain and common gamma-chain. Purified CD4-CD8- cells from thymomas were cultured with the neoplastic epithelial cells, and their differentiation into CD4+CD8+ cells via CD4 single positive intermediates was observed within 9 days' co-culture in the presence of recombinant IL-7. The CD34+CD4-CD8- cells purified from a normal thymus also differentiated to CD4+CD8+ cells in an allogeneic co-culture with the neoplastic epithelial cells of thymoma. In addition, a pleural dissemination from thymoma contained a large amount of cortical thymocytes. These results suggest that the neoplastic epithelial cells retain the function of thymic epithelium and can support T-cell development in thymomas.
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Base de dados:
MEDLINE
Assunto principal:
Timoma
/
Neoplasias do Timo
/
Linfócitos T
Limite:
Adult
/
Female
/
Humans
/
Middle aged
Idioma:
En
Revista:
Pathol Res Pract
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
Japão