Synthetic peptides identify the minimal substrate requirements of tubulin polyglutamylase in side chain elongation.
FEBS Lett
; 459(1): 90-4, 1999 Oct 01.
Article
em En
| MEDLINE
| ID: mdl-10508923
ABSTRACT
The minimal sequence requirement of Crithidia tubulin polyglutamylase is already fulfilled by tubulin-related peptides carrying a free alpha-carboxylate on a glutamic acid residue. Since the product of each glutamylation step fulfills the substrate requirements necessary for the next cycle, very long side chains are generated with brain tubulin as a substrate. Up to 70 mol of glutamic acid was incorporated per alphabeta-heterodimer. We speculate that the strict choice of a particular glutamate residue for the formation of the isopeptide bond initiating a novel side chain is made by a tubulin monoglutamylase which requires the entire tubulin as substrate.
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Base de dados:
MEDLINE
Assunto principal:
Ácido Poliglutâmico
/
Tubulina (Proteína)
/
Crithidia fasciculata
Limite:
Animals
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
Alemanha