Apoptosis in the endothelium of human corneas for transplantation.
Invest Ophthalmol Vis Sci
; 41(10): 2887-93, 2000 Sep.
Article
em En
| MEDLINE
| ID: mdl-10967041
ABSTRACT
PURPOSE:
To determine whether endothelial cell loss of human corneas stored in organ culture before transplantation is due to apoptosis.METHODS:
The corneal endothelium of human corneas, stored in organ culture at 34 degrees C for varying periods of time, were analyzed for the presence of apoptotic cells using the TdT-mediated dUTP nick-end labeling (TUNEL) technique. Corneal endothelial cell apoptosis was confirmed by Hoechst staining and immunolabeling with anti-caspase 3 active antibody.RESULTS:
Apoptotic cells were identified in the corneal endothelium of human organ cultured corneas their number and distribution demonstrated a close correlation with corneal folding and overall quality of the corneal endothelium. TUNEL-positive labeling of cells was confirmed as apoptotic by the presence of morphologic nuclear alterations identified by Hoechst staining and the presence of immunostaining for caspase-3 activity. Corneal endothelial cell apoptosis was independent of cause of donor death, death to enucleation time, and death to culture times.CONCLUSIONS:
Corneal endothelial cell apoptosis appears to determine the suitability of a cornea for transplantation.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Endotélio Corneano
/
Transplante de Córnea
/
Apoptose
Limite:
Adolescent
/
Adult
/
Aged
/
Aged80
/
Child
/
Child, preschool
/
Humans
/
Infant
/
Middle aged
Idioma:
En
Revista:
Invest Ophthalmol Vis Sci
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Reino Unido