Heterologous expression of a mammalian epithelial sodium channel in yeast.
FEBS Lett
; 481(1): 77-80, 2000 Sep 08.
Article
em En
| MEDLINE
| ID: mdl-10984619
ABSTRACT
The alpha and beta subunits of the amiloride-sensitive rat epithelial sodium channel (alpha beta ENaC) were expressed in the yeast Saccharomyces cerevisiae. We used a combination of yeast strains, including a mutant in the secretory pathway (sec6), and Western blotting techniques, to show that alpha beta ENaC was synthesized and targeted through the secretory system to the plasma membrane. Yeasts expressing alpha beta ENaC were more sensitive to salt than the parent strain. In addition, amiloride, a specific blocker of ENaC, was found to suppress salt sensitivity in the yeast strain expressing alpha beta ENaC.
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Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
/
Canais de Sódio
/
Proteínas de Saccharomyces cerevisiae
Limite:
Animals
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Estados Unidos