Immunohistochemical localization of V-ATPases in rat spermatids.
Int J Androl
; 23(5): 278-83, 2000 Oct.
Article
em En
| MEDLINE
| ID: mdl-11012785
ABSTRACT
The sperm acrosome exhibits a low pH. However, the mechanism of acidification in the acrosome remains unclear. Vacuolar-type proton ATPase (V-ATPase) has been shown to play a principle role in generating and maintaining the acidity of organelles such as lysosomes and endosomes. In this study, we examined whether V-ATPase is localized in the acrosome membranes using immunohistochemical techniques. Sections of rat testis were immunostained using antibodies against V-ATPase. Under light microscopic observation, the perinuclear region in spermatids at an early stage of development was heavily immunostained. At the electron microscopic level, gold particles showing the presence of V-ATPase were localized to the acrosome membranes in the developing spermatids. V-ATPase was also localized to the membrane of vesicles locating between the trans-Golgi area and the acrosome. These observations suggest that V-ATPase may play a role in acrosome acidification.
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Base de dados:
MEDLINE
Assunto principal:
Espermátides
/
ATPases Translocadoras de Prótons
/
ATPases Vacuolares Próton-Translocadoras
Limite:
Animals
Idioma:
En
Revista:
Int J Androl
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Japão