Increased expression of major basic protein (MBP) and interleukin-5(IL-5) in middle ear biopsy specimens from atopic patients with persistent otitis media with effusion.
Otolaryngol Head Neck Surg
; 123(5): 533-8, 2000 Nov.
Article
em En
| MEDLINE
| ID: mdl-11077335
ABSTRACT
BACKGROUND:
Molecular biologic evidence to support an etiologic role for allergy in the pathogenesis of persistent otitis media with effusion (OME) is lacking.OBJECTIVE:
The goal of this article was to document expression of allergy-associated Th-2-type cytokines and inflammatory cells in the middle ear mucosa of children with persistent OME.METHODS:
With immunocytochemistry (CD3, major basic protein) and in situ hybridization (interleukin-5 mRNA), middle ear biopsy specimens from 7 children with persistent OME were stained. Nonatopic stapedectomy patients with no history of otitis media served as controls (n = 7).RESULTS:
There was a statistically significant (P< 0.05) difference in expression of CD3, major basic protein, and interleukin-5 between experimental and control subjects. All 8 OME patients proved to be atopic by ELISA testing.CONCLUSIONS:
Type I allergy involving a Th-2-type cytokine and cellular profile may be a contributing factor in the persistence of OME in atopic children.SIGNIFICANCE:
The middle ear may serve as a target organ for allergic inflammation, suggesting that appropriate allergy management may be a useful adjunct to the management of OME.
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Base de dados:
MEDLINE
Assunto principal:
Ribonucleases
/
Otite Média com Derrame
/
Proteínas Sanguíneas
/
Interleucina-5
/
Mediadores da Inflamação
/
Orelha Média
Limite:
Child
/
Child, preschool
/
Humans
Idioma:
En
Revista:
Otolaryngol Head Neck Surg
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Canadá