Fast and reversible trapping of surface glycine receptors by gephyrin.
Nat Neurosci
; 4(3): 253-60, 2001 Mar.
Article
em En
| MEDLINE
| ID: mdl-11224541
ABSTRACT
Variations in receptor number at a given synapse are known to contribute to synaptic plasticity, but methods used to establish this idea usually do not allow for the determination of the dynamics of these phenomena. We used single-particle tracking to follow in real time, on the cell surface, movements of the glycine receptor (GlyR) with or without the GlyR stabilizing protein gephyrin. GlyR alternated within seconds between diffusive and confined states. In the absence of gephyrin, GlyR were mostly freely diffusing. Gephyrin induced long confinement periods spatially associated with submembranous clusters of gephyrin. However, even when most receptors were stabilized, they still frequently made transitions through the diffusive state. These data show that receptor number in a cluster results from a dynamic equilibrium between the pools of stabilized and freely mobile receptors. Modification of this equilibrium could be involved in regulation of the number of receptors at synapses.
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Base de dados:
MEDLINE
Assunto principal:
Membranas Sinápticas
/
Proteínas de Transporte
/
Receptores de Glicina
/
Proteínas de Membrana
Limite:
Animals
Idioma:
En
Revista:
Nat Neurosci
Assunto da revista:
NEUROLOGIA
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
França