Activity and mRNA abundance of Delta-5 and Delta-6 fatty acid desaturases in two human cell lines.
FEBS Lett
; 491(3): 247-51, 2001 Mar 02.
Article
em En
| MEDLINE
| ID: mdl-11240136
ABSTRACT
We analyzed fatty acid biosynthesis in Chang and ZR-75-1 cells. Both cell lines could desaturate and further elongate substrates for Delta-5 desaturase. ZR-75-1 but not Chang cells showed Delta-6 desaturation of 182n-6, 183n-3, 244n-6 and 245n-3. In both cell lines, the mRNA abundance can be related to Delta-5 or Delta-6 fatty acid desaturase activities. These results suggest that desaturase genes could have, at least in part, independent control mechanisms and that Delta-6 desaturase impairment is not specific to any particular step of the fatty acid metabolic pathways, which may diminish the rationale for the existence of at least two distinct enzymes.
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Mama
/
RNA Mensageiro
/
Ácidos Graxos Dessaturases
/
Fígado
Limite:
Humans
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Canadá