Your browser doesn't support javascript.
loading
The failure of different strains of Yersinia pestis to produce lipopolysaccharide O-antigen under different growth conditions is due to mutations in the O-antigen gene cluster.
Prior, J L; Parkhill, J; Hitchen, P G; Mungall, K L; Stevens, K; Morris, H R; Reason, A J; Oyston, P C; Dell, A; Wren, B W; Titball, R W.
Afiliação
  • Prior JL; Defence Evaluation and Research Agency, CBD Porton Down, Salisbury, Wiltshire, UK. prior_jo@hotmail.com
FEMS Microbiol Lett ; 197(2): 229-33, 2001 Apr 13.
Article em En | MEDLINE | ID: mdl-11313139
The lipopolysaccharide (LPS) from eight strains of Yersinia pestis which had been cultured at 28 degrees C appeared to be devoid of an O-antigen when analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. LPS isolated from three of these strains which had been cultured at 37 degrees C also appeared to be devoid of an O-antigen. When the LPS from Y. pestis strain CO92 was purified and analysed by matrix-assisted laser desorption-ionisation time-of-flight mass spectrometry, the observed signals were in the mass range predicted for molecules containing lipid A plus the core oligosaccharide but lacking an O-antigen. The nucleotide sequence of Y. pestis strain CO92 revealed the presence of a putative O-antigen gene cluster. However, frame-shift mutations in the ddhB, gmd, fcl and ushA genes are likely to prevent expression of the O-antigen thus explaining the loss of phenotype.
Assuntos
Buscar no Google
Base de dados: MEDLINE Assunto principal: Yersinia pestis / Genoma Bacteriano / Antígenos O Idioma: En Revista: FEMS Microbiol Lett Ano de publicação: 2001 Tipo de documento: Article
Buscar no Google
Base de dados: MEDLINE Assunto principal: Yersinia pestis / Genoma Bacteriano / Antígenos O Idioma: En Revista: FEMS Microbiol Lett Ano de publicação: 2001 Tipo de documento: Article