Some applications of near-ultraviolet laser-induced fluorescence detection in nanomolar- and subnanomolar-range high-performance liquid chromatography or micro-high-performance liquid chromatography.
J Chromatogr A
; 913(1-2): 253-9, 2001 Apr 13.
Article
em En
| MEDLINE
| ID: mdl-11355820
ABSTRACT
In this work we present some applications of near-UV laser-induced fluorescence (LIF) with micro-HPLC (microHPLC) and HPLC. To test the sensitivity of the detection, we used pyrene and aflatoxins, because both of these molecules exhibit native fluorescence. Then we studied catecholamines derivatized with 1,2-diphenylethylenediamine. The results show that we were able to reach better sensitivity levels than previously described in LIF studies. For catecholamines, a 50-fold increase in sensitivity compared to conventional fluorescence was obtained. These results indicate that LIF detection associated with HPLC or microHPLC can be used to detect very low concentrations of substances that can be excited in the near-UV range after labeling at nanomolar concentrations.
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Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Fluorescência
/
Raios Ultravioleta
/
Cromatografia Líquida de Alta Pressão
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
J Chromatogr A
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
França