Development of an ELISA for the detection of the residues of the insecticide imidacloprid in agricultural and environmental samples.
J Agric Food Chem
; 49(5): 2159-67, 2001 May.
Article
em En
| MEDLINE
| ID: mdl-11368571
ABSTRACT
A competitive enzyme-linked immunosorbent assay (ELISA) for the chloronicotinyl insecticide imidacloprid was developed using a polyclonal antibody produced against a hapten conjugated through the imidazolidine to keyhole limpet hemocyanin. In the standard curve of imidacloprid, an IC(50) of 17.3 ng/mL was obtained using a competitive heterologous system at pH 10. Very low cross-reactivity was found for some structurally related compounds including the insecticide thiacloprid. The high cross-reactivity with a metabolite containing the carbonyl group in the imidazolidine moiety suggests the involvement of its polarity and stereochemical fitness in forming the antibody--antigen complex. The effects of various assay conditions, including organic solvents, detergent content, salt concentration, and pH on the sensitivity were evaluated. High-performance liquid chromatography was run for comparison to validate the ELISA with fortified water samples, the correlation being 0.997-0.998 (n = 15) with a slope of 1.10--1.38. The ELISA turned out to be a convenient tool for monitoring imidacloprid residues in agricultural and environmental samples.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Ensaio de Imunoadsorção Enzimática
/
Resíduos de Praguicidas
/
Imidazóis
/
Inseticidas
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
J Agric Food Chem
Ano de publicação:
2001
Tipo de documento:
Article