On the elastic properties of tetramethylrhodamine F-actin.
Biophys Chem
; 92(3): 201-7, 2001 Sep 18.
Article
em En
| MEDLINE
| ID: mdl-11583836
ABSTRACT
(Iodoacetamido)tetramethylrhodamine disrupts F-actin. At the 11 fluorophore to actin (as monomer) ratio approximately 80% of the protein becomes non-sedimentable. The fluorescent, non-sedimentable actin copolymerizes with G-actin to yield fluorescent filaments. The tensile strength of these filaments changes with the ratio of the fluorescent non-sedimentable actin to the G-actin, being 1.6 pN, 2.9 pN and 3.6 pN at the 1/4, 2/3 and 1/1 ratios, respectively. These tensile strengths are approximately two orders of magnitude lower than those obtained by decoration of F-actin with phalloidin.
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Base de dados:
MEDLINE
Assunto principal:
Rodaminas
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Actinas
Idioma:
En
Revista:
Biophys Chem
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Itália