Crystallization of membrane proteins in cubo.
Methods Enzymol
; 343: 183-99, 2002.
Article
em En
| MEDLINE
| ID: mdl-11665567
ABSTRACT
Our understanding of lipidic cubic phases for the crystallization of membrane proteins has advanced greatly since the inception of the concept in 1996, and the method is becoming well accepted. Several protocols that allow the efficient screening of crystallization conditions and handling of crystals are presented. State-of-the art micro techniques allow a large number of crystallization conditions to be tested using very small amounts of protein, and diffraction quality crystals can be grown in larger volumes in glass vials. In cubo crystallization conditions differ from those employed for detergent-solubilized proteins. Variations comprise the type of lipid matrix, detergent, protein, salt, temperature, hydration, pH, and pressure. Commercially available screening kits may be applied in order to define lead conditions. Once obtained, crystals may be removed from the surrounding cubic phase mechanically, by enzymatic hydrolysis, or by detergent solubilization. We anticipate this set of protocols to be applied successfully to larger, less stable, and noncolored membrane proteins in order to obtain well-diffracting crystals of membrane proteins that have so far evaded crystallization in the detergent-solubilized state.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Membrana
Idioma:
En
Revista:
Methods Enzymol
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Estados Unidos