Deregulation of immunoglobulin gene transcription in the Hodgkin-Reed Sternberg cell line L1236.
Br J Haematol
; 115(2): 326-8, 2001 Nov.
Article
em En
| MEDLINE
| ID: mdl-11703330
ABSTRACT
Hodgkin-Reed Sternberg (H-RS) cells harbour clonal immunoglobulin gene (Ig) rearrangements in almost all cases of classical Hodgkin's disease but lack Ig gene expression. In the H-RS cell line L1236, a somatic mutation of the Ig heavy-chain gene promoter octamer motif has been described as a putative reason for absence of Ig gene expression. We addressed transcriptional activity of this mutated promoter by performing reporter gene studies and gel retardation assays. The results showed that the mutation outside the coding region of the rearranged Ig gene in L1236 cells leads to downregulation of Ig gene expression in H-RS cells.
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Base de dados:
MEDLINE
Assunto principal:
Transcrição Gênica
/
Doença de Hodgkin
/
Genes de Imunoglobulinas
/
Células de Reed-Sternberg
Limite:
Humans
Idioma:
En
Revista:
Br J Haematol
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Alemanha