Generic microcystin immunoassay based on monoclonal antibodies against Adda.
Analyst
; 126(11): 2002-7, 2001 Nov.
Article
em En
| MEDLINE
| ID: mdl-11763082
ABSTRACT
A monoclonal antibody (clone AD4G2) was generated against a common part of microcystins and nodularins, the unusual amino acid Adda [(2S,3S,8S,9S)-3-amino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4E,6E-dienoic acid]. A direct competitive ELISA based on this antibody was developed and the cross-reactivity pattern was measured. Different toxins showed a very similar response. The assay provides therefore a sum parameter of microcystins, nodularins and peptide fragments containing Adda. The IC50 for microcystin-LR was 0.33 microg L(-1) which leads to a detection limit of 0.07 microg L(-1). This is well below the concentration of 1 microg L(-1) proposed by the World Health Organisation (WHO) as the limit for drinking water. Microcystin-LR spiked water samples in the concentration range between 0.1 and 1 microg L(-1) were measured and a mean recovery of 113+/-23% was found. The antibody is well suited for the determination of microcystins in drinking as well as surface water.
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Base de dados:
MEDLINE
Assunto principal:
Peptídeos Cíclicos
/
Poluentes da Água
Tipo de estudo:
Evaluation_studies
Idioma:
En
Revista:
Analyst
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Alemanha