Comparative study on the fractionation of cellulases on some hydrophobic interaction chromatography adsorbents.
J Chromatogr A
; 944(1-2): 211-6, 2002 Jan 25.
Article
em En
| MEDLINE
| ID: mdl-11831755
ABSTRACT
In this work, a comparative study for the fractionation of Trichoderma reesei cellulases on five different hydrophobic interaction chromatography adsorbents (Butyl-Sepharose 4 FF, Phenyl-Sepharose 6 FF, Octyl-Sepharose 4 FF, Epoxy-Sepharose CL-6B and Polypropylene glycol-Sepharose CL-6B) is shown. The influence of the mobile phase composition on the chromatographic behaviour of T. reesei cellulases complex was evaluated using different concentrations of ammonium sulphate in the eluent buffer. A selective separation of beta-glucosidase with two-fold increase in specific activity and good recoveries of cellobiase activity were obtained with Butyl-Sepharose 4 FF and Phenyl-Sepharose 6 FF using 7% (w/v) ammonium sulphate in the eluent buffer. A beta-glucosidase fractionation was also obtained with Epoxy-Sepharose CL-6B, using 13% (w/v) of the salt in the mobile phase.
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Base de dados:
MEDLINE
Assunto principal:
Celulase
/
Cromatografia Líquida
Idioma:
En
Revista:
J Chromatogr A
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Portugal