Differential regulation of mnp2, a new manganese peroxidase-encoding gene from the ligninolytic fungus Trametes versicolor PRL 572.
Appl Environ Microbiol
; 68(4): 2077-80, 2002 Apr.
Article
em En
| MEDLINE
| ID: mdl-11916737
ABSTRACT
A peroxidase-encoding gene, mnp2, and its corresponding cDNA were characterized from the white-rot basidiomycete Trametes versicolor PRL 572. We used quantitative reverse transcriptase-mediated PCR to identify mnp2 transcripts in nutrient-limited stationary cultures. Although mnp2 lacks upstream metal response elements (MREs), addition of MnSO(4) to cultures increased mnp2 transcript levels 250-fold. In contrast, transcript levels of an MRE-containing gene of T. versicolor, mnp1, increased only eightfold under the same conditions. Thus, the manganese peroxidase genes in T. versicolor are differentially regulated, and upstream MREs are not necessarily involved. Our results support the hypothesis that fungal and plant peroxidases arose through an ancient duplication and folding of two structural domains, since we found the mnp1 and mnp2 polypeptides to have internal homology.
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Base de dados:
MEDLINE
Assunto principal:
Peroxidases
/
Basidiomycota
/
Regulação Fúngica da Expressão Gênica
Idioma:
En
Revista:
Appl Environ Microbiol
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Estados Unidos