Analysis of spectinomycin by liquid chromatography with pulsed electrochemical detection.
J Chromatogr A
; 953(1-2): 123-32, 2002 Apr 12.
Article
em En
| MEDLINE
| ID: mdl-12058926
ABSTRACT
Until now, no LC method is described to determine the purity and content of spectinomycin without prior derivatization. A reversed-phase ion-pair LC method using a base deactivated column and pulsed electrochemical detection is described. The mobile phase consisted of an aqueous solution containing 5.8 g/l pentafluoropropionic acid, 1.25 g/l potassium dihydrogen phosphate and 5.5 ml/l tetrahydrofuran. The pH was adjusted to 6.25 using dilute NaOH solution. An experimental design was used to optimize the chromatographic parameters and to check the robustness. The quality of separation was investigated on different stationary phases. The method allows the separation of spectinomycin from its related substances as well as some other components of unknown identity. The total time of analysis is 65 min. A number of commercial samples were examined using this method.
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Base de dados:
MEDLINE
Assunto principal:
Cromatografia Líquida
/
Espectinomicina
/
Eletroquímica
/
Antibacterianos
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
J Chromatogr A
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Bélgica