Compartmentation of enzymes in a microbody, the glycosome, is essential in Trypanosoma brucei.
J Cell Sci
; 115(Pt 13): 2651-8, 2002 Jul 01.
Article
em En
| MEDLINE
| ID: mdl-12077356
ABSTRACT
All kinetoplastids contain membrane-bound microbodies known as glycosomes, in which several metabolic pathways including part of glycolysis are compartmentalized. Peroxin 2 is essential for the import of many proteins into the microbodies of yeasts and mammals. The PEX2 gene of Trypanosoma brucei was identified and its expression was silenced by means of tetracycline-inducible RNA interference. Bloodstream-form trypanosomes, which rely exclusively on glycolysis for ATP generation, died rapidly upon PEX2 depletion. Insect-form (procyclic) trypanosomes do not rely solely on glycolysis for ATP synthesis. PEX2 depletion in procyclic forms resulted in relocation of most tested matrix proteins to the cytosol, and these mutants also died. Compartmentation of microbody enzymes is therefore essential for survival of bloodstream and procyclic T. brucei. In contrast, yeasts and cultured mammalian cells grow normally in the absence of peroxisomal membranes unless placed on selective media.
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Base de dados:
MEDLINE
Assunto principal:
Trypanosoma brucei brucei
/
Tripanossomíase Africana
/
Compartimento Celular
/
Proteínas de Membrana
/
Microcorpos
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
J Cell Sci
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Alemanha