Characterization of the first Bartonella henselae strain isolated from a cat in Italy.
Comp Immunol Microbiol Infect Dis
; 25(4): 217-28, 2002 Jul.
Article
em En
| MEDLINE
| ID: mdl-12135236
ABSTRACT
Bartonella henselae has been identified and characterized for the first time in Italy. A strain, designed Pavia-1, was isolated from the blood of a cat whose owner developed cat scratch disease (CSD). Pavia-1 and two American B. henselae strains (Houston-1, ATCC 49882, type I and strain 269608, UC Davis, type II) were compared by whole-cell fatty analysis (CFA), sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for protein profiles, Western immunoblotting (WB) for reactivity with polyclonal antibodies, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), type-specific 16S rRNA PCRs, and pulsed-field gel electrophoresis (PFGE). Bartonella clarridgeiae (ATCC 51734) was also included for comparison. Pavia-1 was identified as a B. henselae type I. PFGE allowed differentiation between B. clarridgeiae and B. henselae and furthermore, between all the B. henselae strains. The fingerprints of PFGE observed for Pavia-1 were distinct from those of B. henselae type II and also of Houston-1, suggesting that the two type I strains derived from two different clones. These results show the capability of B. henselae to develop genotypic variability between genetically related strains.
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Base de dados:
MEDLINE
Assunto principal:
Infecções por Bartonella
/
Doenças do Gato
/
Doença da Arranhadura de Gato
/
Bartonella henselae
Tipo de estudo:
Prognostic_studies
Limite:
Adult
/
Animals
/
Humans
/
Male
País/Região como assunto:
Europa
Idioma:
En
Revista:
Comp Immunol Microbiol Infect Dis
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Itália