Cellular distribution of a high-affinity glutamate transporter in the nervous system of the cabbage looper Trichoplusia ni.
J Exp Biol
; 205(Pt 17): 2605-13, 2002 Sep.
Article
em En
| MEDLINE
| ID: mdl-12151366
ABSTRACT
Glutamate functions as a neurotransmitter in the central nervous system (CNS) and neuromuscular junctions in insects. High-affinity glutamate transporters are responsible for keeping the resting levels of excitatory amino acids below the synaptic activation threshold by removing them from the extracellular fluid, thereby preventing them from reaching toxic levels. Peptides representing the N- and C-terminal regions of a glutamate transporter cloned from the cabbage looper caterpillar (Trichoplusia ni) were synthesized and used to generate polyclonal antibodies. The antibodies produced immunohistochemical staining in both muscular and nervous system T. ni tissues. Neuromuscular junctions in the skeletal muscles produced the most intense labelling, but no visceral muscle or sensory nerves were labelled. In the CNS, the neuropile of the ganglia, but not the connectives, gave a diffuse staining. Electron microscopical examination of ganglia and neuromuscular junctions showed that the plasma membrane of glial cells, but not that of neurons was labelled, in agreement with the notion that most of the glutamate uptake sites in this insect are in glial cells.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Insetos
/
Sistema X-AG de Transporte de Aminoácidos
/
Mariposas
Limite:
Animals
Idioma:
En
Revista:
J Exp Biol
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Canadá