Enhanced DNA repair of cyclobutane pyrimidine dimers changes the biological response to UV-B radiation.
Mutat Res
; 509(1-2): 221-6, 2002 Nov 30.
Article
em En
| MEDLINE
| ID: mdl-12427541
ABSTRACT
The goal of DNA repair enzyme therapy is the same as that for gene therapy to rescue a defective proteome/genome by introducing a substitute protein/DNA. The danger of inadequate DNA repair is highlighted in the genetic disease xeroderma pigmentosum. These patients are hypersensitive to sunlight and develop multiple cutaneous neoplasms very early in life. The bacterial DNA repair enzyme T4 endonuclease V was shown over 25 years ago to be capable of reversing the defective repair in xeroderma pigmentosum cells. This enzyme, packaged in an engineered delivery vehicle, has been shown to traverse the stratum corneum, reach the nuclei of living cells of the skin, and enhance the repair of UV-induced cyclobutane pyrimidine dimers (CPD). In such a system, changes in DNA repair, mutagenesis, and cell signaling can be studied without manipulation of the genome.
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Base de dados:
MEDLINE
Assunto principal:
Dímeros de Pirimidina
/
Raios Ultravioleta
/
Proteínas Virais
/
Reparo do DNA
/
Endodesoxirribonucleases
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Mutat Res
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Estados Unidos