X-chromosome inactivation during spermatogenesis is regulated by an Xist/Tsix-independent mechanism in the mouse.
Genesis
; 34(4): 257-66, 2002 Dec.
Article
em En
| MEDLINE
| ID: mdl-12434336
ABSTRACT
Transcriptional inactivation of the single X chromosome occurs in spermatogenic cells during male meiosis in mammals and has been shown to be coincident with expression of the Xist gene in spermatogonia and spermatocytes in mice. However, male mice carrying an ablated Xist gene show normal fertility. Here we examined expression from the Xist locus during spermatogenesis in wild-type mice and detected sense (Xist), but not antisense (Tsix) transcripts. In addition, we examined expression and chromatin conformation of X-linked structural genes in meiotic and postmeiotic spermatogenic cells from wild-type and Xist(-) mice and found no differences associated with the absence of a functional Xist gene. These results, along with the formation of a morphologically normal XY body in primary spermatocytes in Xist(-) mice, indicate that a functional Xist gene is not required for X-chromosome inactivation during spermatogenesis and that this process is therefore regulated by a different mechanism than that which regulates X-chromosome inactivation in female embryonic cells.
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Base de dados:
MEDLINE
Assunto principal:
Espermatogênese
/
Cromossomo X
/
Mecanismo Genético de Compensação de Dose
/
Regulação da Expressão Gênica
/
RNA não Traduzido
Limite:
Animals
Idioma:
En
Revista:
Genesis
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Estados Unidos