Extracellular signal-regulated kinases1/2 in neurons of the dorsal motor nucleus of the vagus nerve and nucleus of the solitary tract are activated by noxious visceral stimulus in mice.

ABSTRACT

Phosphorylated extracellular signal-regulated kinases1/2 (pERK1/2) -like immunoreactivity (LI) was enhanced in the neurons of the nucleus of the solitary tract (NTS) and dorsal motor nucleus of the vagus nerve (DMV) 8 min after an intraperitoneal injection of acetic acid in the mouse; the enhancement in pERK1/2-LI was suppressed after vagotomy. With immunofluorescent double labeling technique, the co-localization of acetic acid-induced pERK1/2 and tyrosine hydroxylase-LIs was observed in some of the NTS and DMV neurons. These results suggest that ERK1/2 signal-transducting pathway is involved in neuronal activities in NTS and DMV which are induced by vagus-conveyed nociceptive visceral information, and that some of these pERK1/2- immunoreactive neurons in NTS and DMV are catecholaminergic.