COP9 signalosome components play a role in the mating pheromone response of S. cerevisiae.
EMBO Rep
; 3(12): 1215-21, 2002 Dec.
Article
em En
| MEDLINE
| ID: mdl-12446563
ABSTRACT
A family of genetically and structurally homologous complexes, the proteasome lid, Cop9 signalosome (CSN) and eukaryotic translation initiation factor 3, mediate different regulatory pathways. The CSN functions in numerous eukaryotes as a regulator of development and signaling, yet until now no evidence for a complex has been found in Saccharomyces cerevisiae. We identified a group of proteins, including a homolog of Csn5/Jab1 and four uncharacterized PCI components, that interact in a manner suggesting they form a complex analogous to the CSN in S. cerevisiae. These newly identified subunits play a role in adaptation to pheromone signaling. Deletants for individual subunits enhance pheromone response and increase mating efficiency. Overexpression of individual subunits or a human homolog mitigates sst2-induced pheromone sensitivity. Csi1, a novel CSN interactor, exhibits opposite phenotypes. Deletants also accumulate Cdc53/cullin in a Rub1-modified form; however, this role of the CSN appears to be distinct from that in the mating pathway.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Feromônios
/
Saccharomyces cerevisiae
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Proteínas
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Proteínas de Saccharomyces cerevisiae
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Proteínas Culina
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
EMBO Rep
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Israel