Molecular characterization of a cell wall-associated beta(1-3)endoglucanase of Aspergillus fumigatus.
Med Mycol
; 40(5): 455-64, 2002 Oct.
Article
em En
| MEDLINE
| ID: mdl-12462524
ABSTRACT
A 74 kDa beta(1-3)endoglucanase of Aspergillus fumigatus was recently isolated from a cell wall autolysate and biochemically characterized. In this study, we report the cloning and the disruption of the ENGL1 gene encoding this beta(1-3)endoglucanase. ENGL1 contains an open reading frame of 2181 bp encoding a polypeptide of 727 amino acids. Sequence analysis showed that ENGL1 is the first characterized member of a new family of beta(1-3)glucanases. Disruption of ENGL1, however, did not lead to a phenotype distinct from the parental strain, indicating that this cell wall-associated beta(1-3)endoglucanase does not play an essential role in constitutive cell growth.
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Base de dados:
MEDLINE
Assunto principal:
Aspergillus fumigatus
/
Glucana Endo-1,3-beta-D-Glucosidase
Tipo de estudo:
Risk_factors_studies
Idioma:
En
Revista:
Med Mycol
Assunto da revista:
MEDICINA VETERINARIA
/
MICROBIOLOGIA
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
França