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Interaction between hepatitis C virus core protein and translin protein--a possible molecular mechanism for hepatocellular carcinoma and lymphoma caused by hepatitis C virus.
Li, Ke; Wang, Lin; Cheng, Jun; Lu, Yin-Ying; Zhang, Ling-Xin; Mu, Jin-Song; Hong, Yuan; Liu, Yan; Duan, Hui-Juan; Wang, Gang; Li, Li; Chen, Ju-Mei.
Afiliação
  • Li K; Jun Cheng Gene Therapy Research Center, Institute of Infectious Diseases, The 302 Hospital of PLA, Beijing 100039, China.
World J Gastroenterol ; 9(2): 300-3, 2003 Feb.
Article em En | MEDLINE | ID: mdl-12532453
AIM: To investigate the interaction between hepatitis C virus core protein and translin protein and its role in the pathogenensis of hepatocellular carcinoma and lymphoma. METHODS: With the components of the yeast two hybrid system 3, "bait" plasmids of HCV core the gene was constructed. After proving that hepatitis C virus core protein could be firmly expressed in AH109 yeast strains, yeast two- hybrid screening was performed by mating AH109 with Y187 that transformed with liver cDNA library plasmids-pACT2 and then plated on quadruple dropout (QDO) medium and then assayed for alpha-gal activity. Sequencing analysis of the genes of library plasmids in yeast colonies that could grow on QDO with alpha-gal activity was performed. The interaction between HCV core protein and the protein we obtained from positive colony was further confirmed by repeating yeast two - hybrid analysis and coimmunoprecipitation in vitro. RESULTS: A gene from a positive colony was the gene of translin, a recombination hotspot binding protein. The interaction between HCV core protein and translin protein could be proved not only in yeast, but also in vitro. CONCLUSION: The core protein of HCV can interact with translin protein. This can partly explain the molecular mechanism for hepatocellular carcinoma and lymphoma caused by HCV.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas do Core Viral / Hepacivirus / Carcinoma Hepatocelular / Proteínas de Ligação a DNA / Neoplasias Hepáticas / Linfoma / Proteínas de Neoplasias Limite: Humans Idioma: En Revista: World J Gastroenterol Assunto da revista: GASTROENTEROLOGIA Ano de publicação: 2003 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas do Core Viral / Hepacivirus / Carcinoma Hepatocelular / Proteínas de Ligação a DNA / Neoplasias Hepáticas / Linfoma / Proteínas de Neoplasias Limite: Humans Idioma: En Revista: World J Gastroenterol Assunto da revista: GASTROENTEROLOGIA Ano de publicação: 2003 Tipo de documento: Article País de afiliação: China