[Development of a method for preparing adeno-associated virus type 4 antigen]. / Razrabotka metoda polucheniia antigena adenoassotsirovannogo virusa tipa 4

ABSTRACT

A method for preparation of adeno-associated type 4 virus (AAV-4) purified from group-specific adenovirus antigen by adsorption on formalinized sheep erythrocytes and elution into hypertonic NaCl solution was developed. In 1 M naCl solution the purified AAV-4 retained its infectivity and the complement-fixing and hemagglutinating activities. Separation of AAV-4 and adenovirus group-specific complement-fixing antigen was based on differences in conditions of their adsorption and elution. AAV-4 was inactivated by treatment with both formalin and hydrogen peroxide but retained its complement-fixing antigen and hemagglutinating properties. The purified antigen or virus is recommended for serologic tests and other purposes.