Measurement of phosphatidylcholine hydroperoxide in mild ischemia-reperfusion injury in rat intestine.
Tohoku J Exp Med
; 198(4): 223-32, 2002 Dec.
Article
em En
| MEDLINE
| ID: mdl-12630554
ABSTRACT
In the pathogenesis of intestinal ischemia-reperfusion injury, the measurement of lipid peroxides needs to be established. Sprague-Dawley rat intestines were assessed after 30 minutes of occlusion of the superior mesenteric artery followed by reperfusion at 30, 60, 120, 180, 360 minutes. Grade of the mucosal injury, accumulation of the activated neutrophils and ICAM-1 expression were transiently increased after reperfusion. Two measuring methods of mucosal lipid peroxides using thiobarbituric acid reacting substance (TBARS) and phosphatidylcholine hydroperoxide (PCOOH) were compared. PCOOH level was significantly increased after reperfusion, while the mucosal TBARS level showed no significant change. In conclusion, lipid peroxidation could be detected with high specificity and sensitivity by measuring the mucosal phosphatidylcholine hydroperoxide level.
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Base de dados:
MEDLINE
Assunto principal:
Fosfatidilcolinas
/
Traumatismo por Reperfusão
/
Mucosa Intestinal
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Tohoku J Exp Med
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Japão